引

His Curmudgeon column which ran for many years in the Sunday Independent was required reading and was always guaranteed to raise a smile, he said.

他的坏脾气专栏在星期日独立报里跑有许多年被需要阅读和总是被保证引人发笑，他说。

The order of the factors affecting the teeming time is current intensity, frequency and diameter of the coil.

找出了可以满足出钢要求的最佳电流强度、电源频率和线圈直径参数，实验验证了电磁引流技术可以在工艺要求的条件下完成出钢。

By using Roche kit as standard, the amplification efficiency of different reagents were compared by Roche Light Cycler instrument for establishing real-time PCR reaction system. The primers were designed based on signature sequence of chromosomal DNA fragment and caf 1 gene in plasmid pMT1 of Y.pestis. And sensitivity and specificity assay were done with serial diluted Y. pestis EV76 strain and other bacterias. Then 275 Y.

采用SYBRGreenI随机掺入法，以Roche试剂盒为标准，比较两公司的DNA聚合酶，用鼠疫菌310 0 4建立荧光PCR反应体系；针对鼠疫耶尔森氏菌特异的染色体标识序列和质粒上F1抗原基因设计引物，检测其灵敏度和特异性，以盲测试验进行验证；在此基础上鉴定2 75株鼠疫耶尔森氏菌，并测试该法检测脏器污染模拟标本的灵敏度。

Methods: Two sets of primers were designed according to the cyclostyle of pcDNA3.1-HBV-YVDD, and the full-length of HBV genome with different enzyme digestin spot was amplified.

以pcDNA3.1-HBV-YVDD为模板设计两对引物，扩增带不同酶切位点的HBV全基因，扩增产物纯化后，经酶切、连接，得到pcDNA3.1-HBV-YVDD重组体。

Part four will prove the following 4 theorems : theorem 1 :the curvature and torsion of the locus of foot point of the perpendicular line between the origin and the tangent line of the cylindrical spiral remains to be function t , but not trigonometric function t .

第四部分证明四个定理分别为定理1 由坐标系原点至圆柱螺线的切线所引垂线的垂足的轨迹曲率和挠率仍为t的函数，但不是t的三角函数。

Dr. Johnson -- a man whose mind had as much iron in it as any man's -- protested in a note on Cymbeline which is worth quoting in full.

约翰逊博士——一个不比任何人软弱的人——在《辛白林》一剧的评论中提出的抗议值得全文引下。

Cancer Genetics and Cytogenetics is one of the more frequently cited journals in oncology.

癌基因和细胞遗传学杂志是在肿瘤学中频繁被引的期刊之一。

The cDNA has 752bp and encodes a protein of 216 amino acids. Thenascent Src l contained a signal peptide of 19 amino acids, a propart of 19 amino acidsand a 178-amino-acid mature Src l. The characteristic amphiphilic α-helix of cytolysin islocated at the N-terminus of the mature Src l.

根据文库的EST序列设计引物，从玫瑰红绿海葵克隆到一全长cDNA序列，命名为Src I，利用生物信息学方法对其进行了分析，结果表明，该cDNA序列编码一个216个氨基酸的海葵细胞毒素前体蛋白，它包括19个氨基酸的信号肽、19个氨基酸的propart motif和178个氨基酸的成熟蛋白。

Methods: A polymerase chain reaction assay with oligonucleotide primers homologous to a portion of the urease C and cytotoxin associated gene A of Hp was used to test the presense of Hp in dental plaque.

依据特异的尿素酶C 基因和 cag A基因设计引物，建立聚合酶链反应方法，检测65例慢性胃病患者不同牙齿的龈上和龈下菌斑中的Hp.6例胃Hp阳性的胃炎患者，经药物治疗后，检测口腔Hp。

2085 Pinout: The L Family is a Low Power version of the CH1817 DAA.

52207-2085引脚说明：的L系列是一款低功耗的CH1817的DAA版本。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。