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Five polymorphic primers with good, clear bands were got, screening fromseventy-five ISSR primers. These five pairs of primers were used to amplificaterespectively the genomic DNA of 66 different Pterocarya stenoptera individuals and atotal of 45 bands were amplified with 43 polymorphic bands and percentage ofpolymorphic bands 95.6%.

2从75条ISSR引物中筛选出5条多态性好、谱带清晰的引物，用这5条引物对66个枫杨叶样的基因组DNA进行了PCR扩增，共得到45条带，其中多态性带43条，多态性百分率为95.6％。

Rapid amplification of cDNA end:Retroverse transcription product of total RNA extracted from normal porcine tissue was used as the template,gene specific primers were designed and advantage 2 polymerase mix was used in PCR,of which using porcine genomic DNA as the template:forward primer was designed according to the acquired consensus region of human and pig FGL2 3′ sequences while reverse primer was designed from human FGL2 3′ end downstream sequence;TA cloning.

以猪正常小肠及心脏组织提取新鲜总RNA，反转录后作为模板，设计基因特异性引物，采用Advantage 2 聚合酶混合物进行PCR扩增；依据猪与人FGL2基因3′端已知同源序列设计PCR上游引物，以人FGL2基因3′末端序列设计下游引物，以猪基因组DNA为模板采用Advantage 2 聚合酶混合物进行PCR反应；PCR载体重组质粒DNA亚克隆扩增。

The increased synthesis of PGE2 in the intrauterine tissues is a key of the initination of labor induced by castor oil-diet, and ricinoleic acid in castor oil-diet might be the active component which induced the initiator of labor.

蓖麻油引产餐引起宫内组织PGE2合成变化是其引产的关键，而蓖麻酸可能是蓖麻油引产餐的活性成分。

On the basis of establishing an rat model, the aim of this study was to investigate the effect of castor oil-diet and/or ricinolic acid,the active component of castor oil on the synthesis of PGE_2, on the expression of COX-2 gene (clycloxygenase-2) and on the cervical ripening in pregnant rat.

本研究在建立引产餐妊娠大鼠引产模型的基础上，探讨蓖麻油引产餐和蓖麻油中活性成份一蓖麻酸对妊娠大鼠体内组织前列腺素(PGE_2)合成对前列腺素合成酶环氧化酶-COX2。

Result In the Identification of ISSR molecular markers, 3 primers in 20 of being chose had better amplification effect. Optimization results representative of C. grandis Osbeck showed that primers 826 for the optimum temperature of Rutaceae plants amplification was 52.0℃, and the best cycle time was 35 cycles.

结果］选用的20个引物中有3种引物扩增效果较好，以柚为代表的优化结果表明，引物826对芸香科植物扩增的最适退火温度为52.0℃，最佳循环次数为35个循环。

Gliadin observed secaline specific band in the offspring and its father Lanxiaohei. Screening with 6 pairs of SSR primers on the long arm and short arm of 21 chromosomes, respeetively.

筛选小麦21条染色体长短臂上各6对引物，结果发现只有IBS上的3对引物未扩增出IBS的条带，其余引物均扩增出了各自的相应条带。

The DNA was extracted from mature Spinacia oleracea L.separately by the method of CTAB, then used as template after mixed by gender to be amplified with the optimized RAPD reaction condition. A total of 160 primers (10 bp) and 40 pairs of primers were screened, 44 primers of which produced clear patterns.

菠菜为雌雄异株植物，用CTAB法提取其雌雄成株幼嫩叶片总DNA分别构建雌、雄株的DNA池，以之为模板，用已优化的RAPD体系扩增，在160条随机引物(10 bp)和40个引物对中筛选出44个扩增效果较好的单引物，再从中进行进一步筛选。

An increase in the blood flow velocity of internal jugular vein,the abnormal acoustic frequency and its spectrum of TCD were signs indicated that the blood stream of CCF were drained by the superior and/or inferior petrous sinuses.TCD could detect the superficial sylvian vein where the blood flow velocity increased when the cortical veins were the main veins draining CCFs.

异常眼静脉搏动征，血流反向是诊断CCF眼上静脉引流的可靠指标；颈内静脉流速增高，声频及频谱异常是CCF血流经岩上窦、岩下窦引流的征象；当以皮层静脉引流为主时，颅内常可检出流速增高的侧裂静脉。

The genomic DNA of 31 accessions of genus Tagetes was amplified with 11 primers, the bands produced by each primer ranged from 5 to 11, and 6.8 bands were obtained on average, the polymorphic loci ranged from 40%-100%.

用11个引物对31份万寿菊属材料进行PCR扩增，每个引物扩增的ISSR条带数在5-11条，平均每条引物能扩增出6.8条带，多态位点百分率为40%-100%。

Another pair of primers including the start codern and the terminer coden were designed to amplify the PAL gene. Two restriction enzyme XbaI and XmaI were added to the two primers respectively, which enable to insert this fragment to the prokaryotic expression vetor pCAMBIA2301-WCS.

在PAL 基因的编码框以启始密码子ATG 和终止密码子TAG 为两端设计一对特异引物，分别在引物5' 端引人XbaI 和XmaI 切位点，用来扩增PAL 基因并与pCAMBIA2301-WCS 构建表达载体pCAMBIA2301-PAL。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。