引

TheMADS box locates just at the 5'end of most of genes and is highly conserved.According to these,a primer was designed and the effort to isolate new membersof MADS box gene family in rice genome by RT-PCR was made.

绝大多数已克隆到的这类基因的MADS-box恰好位于5'端，而且非常保守，因此，我们根据MADS box基因的保守区设计了一个特异的引物，试图通过RT-PCR分离出水稻花中MADS box基因家族的新成员。

This is not a criticism of Titus Bramble or Antoine Sibierski, but a negative view of the club's recruitment strategy.

布尔布兰和西贝尔斯基没有可以争论的，但是俱乐部的引援策略却是个不同的景象。

Primers used in PCR were designed based on the sequence of mitochondrial 16S rDNA (AJ250642 and AF312718) and beta-actin (AY910691) of Eriocheir sinensis which had been deposited in GenBank. PCR analysis using total DNA from muscle, branchia, testis and sperm indicated that 16S rDNA exhibited different expression in the four samples. Beta-actin gene was detected plentiful in all the four tissues/cells and exhibited the same expression pattern. 16S rDNA gene amplification was detected at high levels in muscle and branchia, appreciable low in testis and very low in sperm. The PCR products of 16S rDNA gene were sequenced and aligned with the sequence of 16S rDNA from GenBank (AJ250642 and AF3 12718), and the alignment result showed there was no difference between them.

通过GenBank中的中华绒鳌蟹线粒体16S rDNA序列设计引物，利用PCR扩增方法，以beta-actin做内参，检测了中华绒螯蟹肌肉、鳃、精巢和精子中线粒体16S rDNA扩增情况，发现各组织或细胞beta-actin扩增片段大小、条带宽度和亮度基本一致，表明各模板DNA量基本一致；而在同一DNA浓度下，16S rDNA的扩增片段长度虽一致，但其产物量存在明显差异，精子16s rDNA产物量显著低于其他3种组织，其条带宽度和亮度很弱；16S rDNA扩增产物经测序分析及比对证明与已有序列完全吻合。

This study first discovered it is different in nuclear DNA between Ogura-CMS line and its maintainer in the Brassica crop.

根据测序结果设计了1对特异引物，将M6E7-700标记转化为更稳定的SCAR标记。

" While the initial problem was "skin breakdown related to immobility," a new problem is "potential for skin breakdown related to draining wounds.

一个新的问题/诊断被加入到一个目标上如：目标"皮肤完好"所带的初始问题是"和不动相关的皮肤受损"，新的问题是"和伤口引流相关的潜在的皮肤受损"。

The simultaneity use of Xiao Er Chuan Ke Ye and Cang Er Zi San intervening asthma and allergic rhinitis,comparing simply use Xiao Er Chuan Ke Ye,has the predominance in prolonging time of introducing asthma and reducing the frequency of nodding breathe,which indicated that the treatment of allergic rhinitis has great importance in asthma control and lessen the degree of asthma(frequency of rat nodding breath).3,The combination high-dose group has better therapeutic effect than dexamethasone group,all Chuan Ke Ye groups and combination low,medium group in the prevention and control of allergic rhinitis symptoms,reduced airway hyper responsiveness,while in improving breath difficulties combination high-dose group has similar effect with western medicine group,and better effect than all Chuan Ke Ye groups and combination low,medium group.Its efficacy increased with the prolonging of treatment time,but fewer side effects,which makes the application safer and more reliable.4,Xiao Er Chuan Ke Ye combining with Cang Er Zi San can obviously improve the pathological changes in rats with bronchial airway remodeling,reducing the serum TGF-β1,IL-4,ECP levels,increasing the level of serum IFN-γand the imbalance IFN-γ/ IL-4 ratio.

而运用小儿喘咳液合苍耳子散同时干预哮喘与过敏性鼻炎，较之单纯使用小儿喘咳液治疗哮喘，其在延长引喘时间、减少点头呼吸频度等方面有明显的优势，说明过敏性鼻炎的治疗，对于控制哮喘发生，减轻哮喘发作的程度，有着重要的意义。3、大剂量的合方在预防和控制过敏性鼻炎症状、降低哮喘气道高反应性方面疗效较地塞米松对照组、喘咳液各剂量组及合方小、中剂量组更优，而改善呼吸困难方面则与西药组相当，优于喘咳液各剂量组及合方小、中剂量组，且其疗效随着治疗时间的延长而增加，但副作用更少，使用更加安全可靠。4、小儿喘咳液合苍耳子散能明显改善气道重塑大鼠支气管的病理改变，同时降低血清TGF-β1、IL-4、ECP水平，提高血清IFN-γ水平及失衡的IFN-γ/IL-4比值。

We designed primers according to the sequence of Genbank from Bifidobacterium breve and have cloned the putative isomerasekey gene and several fragments from the genome DNA of Lactobacillus acidophilus .

根据Genbank中短小双歧杆菌中可能的共轭化酶的序列资料，设计引物，克隆了可能的基因及几个片段并测序，其基因功能有待进一步验证。

I'll try to bring the conversation around to the subject of money.

我想把谈话引到钱的问题上。

Education is to bring you from darkness to light.

教育是要把你从黑暗引向光明。

Go," said he,"to the tent of Achilles, son of Peleus; take Briseis by the hand and bring her hither; if he will not give her I shall come with others and take her- which will press him harder.

"去吧，速往裴琉斯之子阿基琉斯的营棚，牵回美貌的布里塞伊丝。倘若他不让你们执令，我将亲往带走那位姑娘，引着大队的兵勇，从而大大加重他的悲难。"

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。