引

A new mannose-binding agglutinin gene was cloned from bulbs of Amaryllis vittata Ait. The full-length cDNA of A. vittata agglutinin was 686 bp. The start codon of ava cDNA was at 41-43 bp and the stop codon was at 515-517 bp.

运用同源克隆的方法设计简并引物，通过3'和5'RACE技术，从石蒜科植物朱顶兰总RNA中克隆了编码此凝集素的全长cDNA序列。

A new mannose-binding agglutinin gene was cloned from bulbs of Amaryllis vittata Ait. The fulllength cDNA of A. vittata agglutinin was 686 bp. The start codon of ava cDNA was at 41-43 bp and the stop codon was at 515-517 bp.

运用同源克隆的方法设计简并引物，通过3′和5′RACE技术，从石蒜科植物朱顶兰总RNA中克隆了编码此凝集素的全长cDNA序列。

He is proud that his wife is amenable to reason.

他的妻子是个通情达理的人，为此他引以为豪。

Elegant it is not the most compelling visual amenity of the matter, is exactly the kind affinity.

优雅 优雅最引人悦目的不是它的高不可攀，恰是平易近人的亲和力。

It is among the truths believed to be self-evident by the followers of all religions that godlessness is equivalent to amorality and that ethics requires the underpinning presence of some sort of ultimate arbiter, some sort of supernatural absolute, without which secularism, humanism, relativism, hedonism, liberalism and all manner of permissive improprieties will inevitably seduce the unbeliever down immoral ways.

要知道所有的宗教追随者，都认为这是不证自明的：那就是不承认神的存在，就等同于缺乏道德观念。而且伦理需要有某种形式的最终仲裁者，某种形式的超自然的绝对存在作为支柱支撑着。如果没有这支柱存在，那么世俗主义，人权主义，相对主义，享乐主义，自由主义以及所有的无拘束的不当行为，会不可避免地将非教徒引上堕落的道路。

A pair of specific primers was designed from the sequenceand was used to amplify the genomic DNAs of Yangmai 5,6VS/6AL line,substitution line of 6V and amphidiploid of Triticum durum-Haynaldiavillosa.

根据该序列设计了一对特异引物，分别以感病对照扬麦5号，抗病6VS/6AL易位系，6V代换系，硬粒小麦-簇毛麦双二倍体及簇毛麦的基因组DNA为模板进行特异性扩增。

Studies on sequence changes in the early genomes of interspecific F_1 hybrid and amphidiploid in CucumisIn the present study,extensive genomic changes were detected in newly obtained allotetraploid of Cucumis,namely Cucumis hytivus Chen and Kirkbride,by AFLP analysis using 12 pairs of EcoR-I/Mse-I primers.

甜瓜属远缘杂种F_1及人工异源双二倍体早期基因组序列变化研究利用12对Eco RI/Mse I选择性引物，对甜瓜属人工异源双二倍体C.hytivus及其二倍体父母本进行AFLP分析，结果发现多倍体基因组在形成早期发生了广泛的序列变化。

In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

But in fact, as Carnegie so ably and amusingly points out through his many examples, worry makes no sense at all.

但实际上，就如同Carnegie通过他的很多例子，成功并引人发笑地指出的那样，忧虑根本毫无意义。

Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭质粒YCEp1为载体构建西方许旺酵母部分基因组文库，在大肠杆菌中扩增后提取混合质粒DNA，经电转化非缺陷标志酒精酵母 AS.2.1364，在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力筛选含水解淀粉的阳性转化子，从阳性转化子中分离重组质粒证实含5.0kb的插入片段，用α-淀粉酶基因两端序列设计的引物PCR扩增及扩增片段序列分析证实该片段中含有α-淀粉酶全部编码序列。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。