引

By conducting broad field investigation, we have found 5 wild populations near the boarder of Yunnan and Sichuan province.

从设计的42对引物中筛选出有效扩增引物28对，其中表明多态性的引物有17对。

SAK-C167CR-LM easily expands data bus width to 32 bits or more using the Master/Slave select when cascading more than one device M/S = VIH for BUSY output flag on Master M/S = VIL for BUSY input on Slave BUSY and Interrupt Flag On-chip port arbitration logic Full on-chip hardware support of semaphore signaling between ports Fully asynchronous operation from either port LVTTL-compatible, single 3.3V (0.3V) power supply Available in 84-pin PGA, 84-pin PLCC and 100-pin TQFP Industrial temperature range (-40C to +85C) is available for selected speeds

朔C167CR - LM滚动轻松扩展数据总线的宽度为32位或更多的使用主/从选择当级联多个器件M / S的=关于硕士M / S的=忙输出标志VIH VIL数值就投入繁忙和忙碌的奴隶中断标志片全部港口的仲裁逻辑芯片上的信号灯的信号港口之间的异步操作完全由硬件支持任何港口的LVTTL兼容，（为0.3V）电源可在84引脚PGA的，84引脚PLCC和单3.3V 100引脚TQFP工业温度范围（- 40C至+85 C）是选定的速度可

MBRD1035CTLT4G easily expands data bus width to 32 bits or more using the Master/Slave select when cascading more than one device M/S = VIH for BUSY output flag on Master M/S = VIL for BUSY input on Slave BUSY and Interrupt Flag On-chip port arbitration logic Full on-chip hardware support of semaphore signaling between ports Fully asynchronous operation from either port LVTTL-compatible, single 3.3V (0.3V) power supply Available in 84-pin PGA, 84-pin PLCC and 100-pin TQFP Industrial temperature range (-40C to +85C) is available for selected speeds

1第1页，本页显示记录1-11，共11条记录分1页显示MBRD1035CTLT4G轻松扩展数据总线的宽度为32位或更多的使用主/从选择当级联多个器件M / S的= VIH对奴隶繁忙投入繁忙和中断标志对主M / S的= VIL数值忙输出标志上港口全部仲裁逻辑芯片的片上信号信号港口之间的异步操作完全由硬件支持任何港口的LVTTL兼容，（为0.3V）电源可在84引脚PGA巡回赛，84引脚PLCC和100引脚TQFP封装3.3V单工业温度范围（- 40C至+85 C）是选定的速度可

Based on analysis of Wx of D4063-1, indica and japonica and according to the special sites of the three species, primers as markers-AUT4063-I were designed to distinguish D4063-1 from other rice. Combining with primer pair F5, dominant and codominant ways were established for discriminating them, and rapid and correct identification of D4063-1 from other rice could be done.

本研究还根据D4063-1和籼稻、粳稻的序列差异及D4063-1在该片段上的特征序列位点设计了用于识别D4063-1的寡核苷酸片段，并作为PCR反应的引物命名为AUT4063-1，将该引物与作者设计的扩增普通籼稻、粳稻Wx基因的引物F5配合使用，建立了识别D4063-1的显性和共显性两种检测方式的分子标记，为快速、准确鉴别低直链淀粉含量突变体D4063-1创造了条件。

In order to achieve a tradeoff between vectoring and unvectoring state, the proposed parameters of DTN are: Z=3~4, secondary injection flux is about 3%, cavity divergence angle is about 10°, cavity convergence angle is 20°~30°, injection angle of secondary is about 30° by counterflow injection.

为了实现DTN在推力矢量和非推力矢量下都有较好的内流综合性能，所建议的设计参数为：落压比为3~4，引射量为3%，凹腔扩张角为10°左右，收敛角在20°~30°，引射角度为30°逆流引射角(β=30°)。

Asynchronous loading of control parameters · Rapid (25ns per pixel) rotation, warping, panning, and scaling of images · Three-dimensional image addressing capability · General third-order polynomial transformations in two dimensions on-chip · Three-dimensional transformation of up to order 1.5 also supported · Flexible, user-congurable pixel datapath timing structure · Static convolutional ltering of up to 16 x 16 Pixel, 256 x 256 pixel or 256 x 256 x 256 pixel windows · User-selectable source image subpixel resolution of 2-8 to 2-16 · Pin-compatible upgrade to TMC2302 · 24-bit (optional 36-bit) positioning precision within the source image space, 48-bit internal precision · Low power CMOS process · Available in a 120-pin Plastic Pin Grid Array and 120-lead Metric Quad Flat Pack

1第1页，本页显示记录1-11，共11条记录分1页显示·异步控制参数加载·快速（25ns的每个像素）旋转，弯曲，平移，缩放和图像·三维图像处理能力·一般二维三阶多项式变换片·三高达1.5三维变换命令也支持·灵活，用户可配置的数据通道的静态时序结构·高达16 × 16像素滤波卷积像素，256 × 256像素或256 × 256 × 256像素（3通）窗口·用户可选的源图像亚像素分辨率的2-8 2-16·引脚兼容的升级TMC2302·24位（可选36位）的定位源图像的空间内，48位内部高精度·低功耗CMOS精密加工·提供120引脚塑料针栅阵列和120引脚公制四方扁平封装

SSRs are very suitable for research on genetic diversity and phylogenetic relationship since they are more reliable, co-dominant in heritance large number of alleles per locus, and abundance in genomes.

对椰子SSR反应体系进行了优化，确立了最适宜的体系：在20μL反应体系中，Mg2+、引物和dNTP的最适浓度分别为2.5mmol/L、0.3μmol/L、0.2mmol/L；TaqDNA聚合的最佳使用量为1U，模板DNA应加入50ng，引物最佳退火温度比每对引物Tm值较小者小2-3℃。

In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

为了获取这一基因片段与国外分离株进行同源性比较，同时也为了了解MDPV强、弱毒株VP2基因之间的异同关系，及其免疫原性的变化规律，通过DNA重组技术，设计了一对引物LHMP7／LHMP8，该对引物选取位于2885～2900及4618～4604的两段序列，跨幅为1734bp，并在这两条引物中分别加入两种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点，分别对MDPV-Q和由该株病毒经人工致弱后得到的MDPV-26株进行PCR扩增，并将PCR产物克隆到pMD18-T载体上，分别得到二个重组子：pMD18-T—M-Q VP2和pMD18-T-M-26 VP2。

Results All six strains amplified by the primers FF showed same detection rate with 021. Sensitivity test indicated that DNA of 1 oocyst per gram of feces could be detected by the PCR.

结果 引物FF扩增 6株C 。parvum均能产生预计 6 0 3bp的片段，引物FF和引物 0 2 1粪样检测结果完全一致，其敏感性是可检出 1个卵囊的DNA。

A band of 850 bp was generated in the line of non-parthenocarpy by the primer N92 both in the parent and in the DNA pool. Accordingly, it is possible that the primer N92 might be used to phenotyping the trait of cucumber parthenocarpy, and further we calculated the genetic distance between the marker and cucumber parthenocarpy trait that might be 18.9 cM.

用65条ISSR引物进行PCR扩增筛选，其中引物N92在亲本和DNA池之间扩增出一条大小为850bp多态性片段，经F2代群体135个单株验证，该片段稳定出现，在单性结实植株中表现为无带，在非单性结实植株中表现为有带，可以作为鉴别单性结实与非单性结实的标记引物。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失