引

Methods Using the well-conserved regions of the DNA polymerase gene in human herpesviruses,we synthesized two pairs of primer,including one pair of primer designed to amplify herpes simplex virus type 1 and 2,Epstein-Barr virus and cytomegalovirus,another pair of primer to varicella-zoster virus and human herpesvirus 6 by PCR.

2 0 0 2年 1～ 12月浙江大学儿童医院在疱疹类病毒高度同源序列DNA多聚酶基因中设计两对通用引物，该引物能同时扩增单纯疱疹病毒Ⅰ型与Ⅱ型、爱泼斯坦—马尔病毒、人巨细胞病毒、水痘带状疱疹病毒和人类疱疹病毒 6型(HHV6) 6种病毒。

The thesis designs and fulfills pole traction and wind control system and summarizes the debug method and advantage of this system through research into upward continuous casting system.

上引连铸设备是铜材冶炼行业中开发和研究的热点，通过对铜杆上引连铸机组的研究，设计并实现了铜杆牵引和卷绕控制系统，并简述了其调试方法及优势。

After purification and quantification,1 ugof total RNAs were reverse transcribed and amplified by PCR with5'upstream 10 base random primers and one-base or two-base anchored 3'primer respectively,and the amplified products were displayed on asequencing gel side by side.

本文以父本黄早4，母本U8112和杂种一代掖单4号的玉米幼苗为材料，提取总RNA，用5'端随机引物和3'端单锚定或双锚定引物，进行了mRNA的差异展示研究。

Methods Firstly, total DNA in the root tuber of P. multiflorum from various habitats was extracted. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and sequenced after purification.

从来自不同原产地的何首乌中提取总DNA，以核基因组ITS通用引物为引物进行扩增，扩增产物经纯化后，用PCR产物直接测序法进行测序。

The Tide-generating forces may be resolved into tidal constituents, the most important being those due to the moon and the sun.

引潮力可以分为几个分量，其中最重要的是那些由于月亮和太阳引起的引潮分量。

The lightning rod leads thunder and the strong thunder and lightning effect is very big to the threat of equipments safe movement,Lightning rod, lead the design of logging out, connecting a ground of pole with arrange direct ...

避雷针引雷，强大的雷电效应对设备安全运行的威胁很大，避雷针、引下线、接地极的设计和布置直接影响设备运行质量。本文以ZQZ-AZ型自动气象站为例，讨论原防雷结构的不足，提出改进的办法。

Chapter 4 is devoted to the existence and multiplicity of homoclinic for self-adjoint nonlinear second order difference equations by using "Mountain Pass " theorem and "Symmetry Mountain Pass "theorem in critical point theory.

第四章应用临界点理论中的山路引理与对称山路引理研究了一类自共轭非线性二阶差分方程的同宿轨的存在性与多重性。

Subtractive hybridization was performed by annealing an excess of driver cDNA with each sample of adaptor ligated tester cDNA. The cDNAs were heat denatured and incubated in 68℃ for 8 hours.

杂交产物经过LA Taq DNA聚合酶补平末端后，以试剂盒提供的PCR Primer 1作为第一次PCR的引物，以Nested Primer 1和Nested Primer 2R作为第二次PCR的引物，通过抑制性PCR富集大量在Tester中高表达的差异表达基因的cDNA。

XLR pin 2 hot/tip, pin 3 -/ring, pin 1 ground/sleeve

平衡针2热/小费，引脚3 -/ring ，引脚1地面/套

A64c $F Head$H $P First lead of the right-side leadGroup is not straight.

F 头 $H $P 右侧引脚组靠上第一个位置引脚不直。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。