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The average contents of A, T, G and C were 14.1%, 16.1%, 30.2% and 39.6%, respectively, the contents of GC 69.8%） were obviously higher than those of AT. Using these primers, ITS-1 region can be amplified in other four Lutjanus species, but the lengths of ITS-1 were largely different between different species.

将此引物在笛鲷属其他4种鱼类中扩增，发现该对引物有很好的通用性；比较发现在不同种中ITS1存在着较大的差异，适合将其应用于分子系统学和种质资源方面的研究。

Treatment options for symptomatic lymphoceles include percutaneous drainage, percutaneous aspiration combined with the installation of a sclerosing agent (bleomycin, doxycycline, povidone iodine, alcohol, or talc),13,15,17 or surgical drainage via open or laparoscopic surgery.1214 Open surgical internal marsupialization of a lymphocele has long been the historical gold standard therapy.

有症状的淋巴管瘤处理选择包括经皮引流、经皮抽吸合并注入硬化剂（博来霉素、多西环素、聚维酮碘、酒精或滑石粉）或通过通过开放或腹腔镜手术术中引流。长期以来，开放性内部袋形缝术一直是治疗淋巴管瘤的金标准。

Statistics show that Mahonia plant has high genetic diversity, and the distance of gene of same species with different place of origin is markedly smaller than that of different species. The species can be identified by comparing the distance of gene between populations with that of database.

统计结果显示：十大功劳属植物具有很高的遗传多态性，在研究的30个随机引物中，多态性比率为95％；不同产地的同种植物的遗传距离明显小于不同种；在应用中可望对待鉴定材料进行相同引物的扩增，引入数据库，通过计算样品与数据库标准样品的遗传距离来对待测样品进行鉴别，或直接通过聚类来确定其归属。

Twelve ISSR primers screened from 100 primers, were selected to analysis the genetic relationship of 22 materials belonging to 16 mango Mangifera indica L.

从100条ISSR引物中选取12条多态性好的引物，对广西亚热带作物所选育的16个杧果Mangifera indica L。

Abstract］ objective to explore the failure causes of mastoidectomy and precautions.methods the operation findings of 37 cases who underwent revision mastoidectomy from 1995 to 2005 were analyzed for causes of the primary procedures and revision mastoidectomy failure.results original symptoms disappeared.all cases achieved a dry ear after mastoidectomy 1～3 months.conclusion causes of failure of mastoidectomy include unfamiliarity with regional anatomy,absence of skill of surgeons,unadequate opening of mastoidale,and clinical experiences.each of these can result in residual lesion and inadequate drainage.some exceptions can also result in reoperation.to resect the pathological changes tissue thoroughly,to remain good drainage after operation,to manage some execptions are keys to prevent reoperation.

目的 探讨乳突根治术不能干耳原因及预防措施。方法对我科1990~2005年收治的37例乳突根治术不能干耳患者的再次乳突手术所见进行分析，并行二次乳突根治术。结果术后1～3个月患者原有症状消失、干耳。结论乳突根治术失败原因主要是术者中耳乳突解剖不熟、基本功不过硬、临床经验不足导致病灶残留、引流不畅和乳突尖开放不够及某些特殊情况所致。术中彻底去除病灶，保证术后引流通畅及特殊情况的处理，是防止二次手术的关键。

METHODS:A PCR protocol was designed to detect the Schistosoma japonicum 5D gene encoding an immunogenic miracidial antigen in cercariae、eggs and adult worms.

通过引物1、引物2单扩增，即能检测到单个虫卵、单个尾蚴或针尖大小的成虫组织。采用Nest－PCR和非同位素探针能将敏感性进一步提高。

Acerifolia. Here, 16 primers out of 38 ISSR primers screened, were found to produce clear reproducible bands resulting in a total of 103 distinct bands, Of these 103 bands, 86 were monomorphic across all 20 of the plants tested and 17 showed polymorphisms (16.5% polymorphism).

研究结果表明：从38条ISSR引物中筛选得到16条引物可以产生明亮清晰、重复性高的条带，条带总数为103条，其中86条条带表现为一致性，在20个快繁苗中无任何差异；而剩下的17条条带表现为多态性(多态率16.5％)。

These primer combinations produced a total of 1 119 bands, 229 specific loci (including 54 absent bands, and 175 monomorphic bands). Among them, 983 polymorphic bands, accounting for 88%, were detected. The percentage of identification per primer combination was as high as 100%. The average PPB of 14 foreign cultivars was 35.86% and the average PPB of seven domestic cultivars was 31.51%.

结果表明：8对引物共产生1 117条谱带， 229个特异位点（其中缺失带54条、单态带175条），多态带983条，多态带的比例为88%；每对引物鉴别效率为100%。14个国外银杏观赏品种平均多态带的比例35.86%，7个国内品种平均多态带的比例31.51%。21个观赏品种之间相似系数为0.4899～0.8499。

The result showed that: 148 poly morphic hands were detected by use of, 22 RAPD primer and 158 allele were detected by use of 34 SSR primer.

利用筛选出的22条RAPD引物，检测到了148条有多态性的带；利用筛选出的34对SSR引物，检测到158个等位基因。

Four kinds of approaches are benefit to these malignant tumor patients, one step's percutaneous nephrostomy is especially safe and effective.

4种引流方法对恶性肿瘤患者的肾功能恢复均有积极作用，尤其是一步法经皮肾穿刺造瘘引流更有临床实用价值。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐