引

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段，应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段；在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法；检测方法的特异性试验结果表明，该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应；检测方法的敏感性试验结果表明，通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg；对20份临床样品检测中，有10份为AIV阳性，其中有5份为H5亚型病毒阳性。

22 Of 22 pairs EST-SSR primers from Chinese cabbage/rape respectively had amplification products in soybean,wheat and maize,the rate of amplification was 81.8%,95.4%,100%,respectively;10 of 22 pairs EST-SSR primers showed polymorphism in monocotyledons wheat and maize,13 in dicotyledons oilseed rape and soybean,7 in both monocotyledonous and dicotyledonous crops.

（1）30对小麦EST-SSR引物中，有29，28和26对引物分别在玉米、油菜和大豆中有扩增产物，可扩增率分别为96.7%，93.3%和86.7%；其中分别有12对和9对引物在单子叶作物小麦和玉米及双子叶作物大豆和油菜中的扩增产物显示多态性，有4对引物在4种作物中的扩增产物均显示多态性。

Case Report: In this report, we present a case of uterine rupture in a 32-year-old multiparous woman after the trial of labor during which 100μg misoprostol was placed in the posterior vagina fornix.

病例报告：一位32岁，妊娠39周（G3P1A1）的妇女因落红而至本院引产，产程一切正常，住院时的Bishop score为0分，因而使用100μg misoprostol 阴道塞剂来引产，在引产过程中，胎儿情况良好，在引产5小时后，就进入产房准备生产，后因fetal distress安排紧急剖腹生产，产下一男婴，3710g，3→4但在术中发现子宫的右侧壁有破裂的情形，而紧急以Vicryl 1°缝合子宫，很幸运地在缝合后很快的控制住子宫的出血。

In order to diagnose disease caused by muscovy duck parvovirus rapidly and accurately, and at same time, further study the taxonomic relationship between muscovy duck parivovirus and goose parvovirus, two pairs of PCR primers (LHMP1/LHMP2 and LHGP1/LHGP2) were designed respectively base on complete nucleotide sequences of Muscovy duck and Goose parvoviruses registered in the GenBank, The LHMP1 for the upper one and the LHMP2 for the lower one, corresponding to the specific nucleotides sequence 454-472 and 1173-1155 respectively in MDPV, and the LHGP1 for the the upper one and the LHGP2 for the lower one, corresponding to the specific nucleotides sequence 454-472 and 1173-1155 respectively in GPV.

为了能快速、准确地对番鸭细小病毒病进行诊断，同时为了能进一步弄清番鸭细小病毒与鹅细小病毒在分类学上是一种怎样的关系，比较GenBank中MDPV和GPV的基因序列，针对这两种病毒左阅读框编码非结构蛋白基因序列的相同区段，分别构建两对PCR引物。第一对引物LHMP1／LHMP2是根据MDPV的基因序列设计的，上游引物针对MDPV基因序列的454～472；下游引物针对基因序列的1173～1155，为19个碱基，扩增跨幅为719bp。

A chemical reaction mechanism model has been established to simulate the combustion process in the cylinder of dual fuel engine. The pilot fuel model has been given to calculate the distribution of the liquid fuel and time of fuel pre-combustion, at the same time, analyzing the important action to the combustion of the natural gas in the combustion chamber of engine, determining the best quantity of pilot fuel.

理论方面，提出了基于化学反应的燃烧模型—多组分气相反应系统的化学反应动力学模型，以此进行双燃料发动机的热力学—化学反应动力计算；引燃油燃烧模型，计算引燃油液滴的分布规律和滞燃期，验证引燃油在双燃料发动机燃烧过程中的作用，揭示不同喷油规律对双燃料发动机的影响，确定在不同负荷下的合理引燃油量；爆震模型，揭示爆震与工质能量释放间的关系和影响爆震的各种运转因素，确定合理的爆震依据，减小爆震倾向。

But the 10 cases of control group were not healed within 2 weeks.Conclusions:VSD as a whole infective surface drainage technique,the drainage tube was uneasy blocked;the manipulation of VSD was mastered easily;the effects were reliable for the treatment of the pyogenesis in diabetes mellitus.

负压封闭式引流是一种全创面引流法，具有引流管不容易被堵塞，操作简便易行等优点，对糖尿病合并的难愈性化脓感染创面引流效果好，创面愈合快，值得推广。

The RAPD was used to study the genetic diversity of 19 KinXin series potato cultivars. The result showed that 52 RAPD bands were amplified using 7 primer, among them 43 bands were polymorphic, and 4~9 bands were amplified in each lane. The RAPD analysis indicated that the genetic distance of 19 potato cultivars was between 0.17~0.72, average value was 0.39. When all of the polymorphic bands were analysed by cluster analysis, 19 potato cultivars were classified into 3 groups. The analysis showed that there existed close sibship among the majority of potato cultivars, and the genetic distance between the minority cultivars and the others is longer, which indicate that the genetic basis of KeXin series potato cultivars is wide.

利用RAPD标记技术对19份克新系列马铃薯品种进行了遗传多样性分析，分别提取19份马铃薯品种的DNA，进行随机引物多态性扩增，从1000条随机引物中初步筛选出7条有多态性的引物进行详细研究，每条RAPD引物扩增出4~9条带，共获得52条带，其中多态性条带为43条；19份马铃薯品种的遗传距离介于0.17~0.72之间，平均值为0.39，平均遗传距离介于0.31~0.51之间；聚类分析结果在GS=0.53处可将克新系列马铃薯品种划分为三类，聚类结果与系谱分析基本相符，同时也说明克新系列马铃薯的遗传基础有所拓宽。

objectiveto explore the drain way to tear with inferior lacrimal passage ruined critically.methodsthe special silicon tube whose bore 1mm and diameter 2mm was inserted between conjunctival sac and nasal vestibule,the tube was placed under the soft tissue of bucco-nasal groove and in the surface of the protrusion of upper jaw bone.resultsthere were 32 patients who undergone tube above five years.one patient of them was pulled out the tube because of papilloma hyperplasia,the other patient was pulled out the tube with the tube slide below,and the rest 30 patients kept the drain well and co

目的探讨下泪道破坏严重时泪液引流途径。方法在结膜囊-鼻前庭间安置一个内径1mm、外径2mm的特制硅胶导管，此管置于鼻颊沟软组织下，上颌骨前突表面。结果治疗32例，观察5年以上，其中1例因结膜乳头状瘤增生而拔管，1例因导管下滑而拔管，30例引流良好，病人亦能耐受。本文就引流机制、并发症、机体耐受问题和操作进行了详细地讨论。结论泪道外插管是下泪道严重损害时泪液引流的一种较好的解决方法。皮下；结膜鼻腔造瘘术；硅胶导管

Chapter five discusses the design and realization of the Pin, the Terminal and the supporting function of the control to the line.

第五章讲述控件引脚、端口及对连线支持功能的设计和实现，详细讨论了引脚和端口的外观设计、引脚间的数据传递功能、控件引脚的添加和删除功能、控件对连线支持功能的设计和实现。

Results: Among 80 cases of ACST, 76 cases were successfully implemented LC, gallbladder puncture and decompression choledocholithotomy T-tube drainage were performed in 11 cases, choledocholithotomy T-tube drainage in 15 cases, common bile duct gall bladder fistula Toishi T-tube drainage in 14 cases, gallbladder removal choledocholithotomy T-tube drainage in 36 cases, operative time 15-120 min, bleeding 10-80 ml; 4 cases transitted laparotomy, 1 case of gallbladder and surrounding tissue adhesion serious, ampulla signs disappear, and the hepatoduodenal ligament ill-defined, 2 cases of gallbladder contraction deep within the liver, gallbladder triangle scar adhesions, and 1 case of duct stone impaction and severe adhesions; postoperative hospital stay was 8-11 d, all were well recovered; pathological diagnosis, acute cholecystitis in 41 cases （51.2％）, acute suppurative cholecystitis in 24 cases （30.0％）, acute gangrenous cholecystitis in 15 cases （18.8％）.

结果：重症急性胆囊炎80例，76例成功实施LC，其中，胆囊穿刺减压胆总管切开取石T管引流11例，胆总管切开取石T管引流15例，胆囊造瘘胆总管切开取石T管引流14例，胆囊切除胆总管切开取石T管引流36例，手术时间15～120 min，术中出血10～85 ml；4例中转开腹，其中，1例胆囊与周围组织粘连严重，壶腹部标志消失，与肝十二指肠韧带界限不清，2例胆囊萎缩深陷肝脏内，胆囊三角区瘢痕粘连，1例胆囊管结石嵌顿且严重粘连，术后住院时间8～11 d，术后恢复均良好，治愈出院；80例术后病理诊断，急性单纯性胆囊炎41例（51.2％），急性化脓性胆囊炎24例（30.0％），急性坏疽性胆囊炎15例（18.8％）。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。