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The PCR products appeared to be approximate 600 bp, the expected size. The allantoic fluid of infected embryonating eggs of 6 IBV reference strains and 6 field strains were amplified by RT-PCR using three pairs of primers of 1.7, 0.2, 0.6 kb on same and different condition, 3, 5, 9 strains of IBV were amplified respectively. While 12 different sentypes of the 12 IBV strains can be divided in 6 genotypes.

用1.7、0.2、0.6 kb 3对引物对6个IBV毒株和6个分离株的含毒尿囊液在相同和不同条件下进行RT-PCR，结果3对引物分别扩增出3、5、9株IBV，同时可将不同血清型的12个IBV株分成6种基因型。

80 Individuals of ATA-A F2 plants were used for RAPD molecular mapping of the monoecious gene by bulk segregant analysis. 840 random primers were chosen for polymerase chain reaction amplification between monoecious pool and andromonoecious pool, the results showed that: primer S128 was tightly linked to the monoecious character, and primer S493 was tightly linked to the andromonoecious character.

以甜瓜单性花系ATA-A系与两性花品种YJX-1的杂交F2代的80个单株为材料，利用群分法进行单性花基因有关RAPD分子标记研究，筛选分析了840个10bp随机引物的PCR扩增谱带，结果表明，随机引物S128在1180bp，S493在930bp处产生与性别相关的特异性条带，S128的特异条带与单性花性状相关，S493的特异条带与两性花性状相关。

Results The left side of caudate lobes varied little in the shape and border,having a constant pedicle of vessels and bile ducts, which could be easily resected in surgery.Most of the blood vessle branches and bile duct of caudate lobe arose from the anterosuperior edge of Glisson's cap-sule.

结果 ①尾状叶左侧的形态、大小、边界变异不大，其血液及胆管引流有一个相对固定的&蒂&，手术切除比较容易；②尾状叶的血管供应、胆管引流繁杂，但均连接于Glisson's管的一、二级分支的前上缘；③肝短静脉数量、注入部位变异大，其走行多与腔静脉壁垂直。

Results The left side of caudate lobes varied little in the shape and border,having a constant pedicle of vessels and bile ducts,which could be easily resected in surgery.Most of the blood vessle branches and bile duct of caudate lobe arose from the anterosuperior edge of Glisson's cap-sule.The hepatic short veins varied significantly in number,but all veritcal to inferior vena cave.Conclusion There are four approaches for cau

结果 ①尾状叶左侧的形态、大小、边界变异不大，其血液及胆管引流有一个相对固定的&蒂&，手术切除比较容易；②尾状叶的血管供应、胆管引流繁杂，但均连接于Glisson's管的一、二级分支的前上缘；③肝短静脉数量、注入部位变异大，其走行多与腔静脉壁垂直。

Studies have shown that compared with ordinary fly ash, more air-entraining agent is added to achieve the same antifreezing performance when using mixed ash to produce concrete.

研究表明，与普通粉煤灰相比，利用混合灰生产混凝土时需要加更多的引气剂才能达到相同的抗冻性能，且随生物质配比的增加，所需引气剂的量也相应增加。

An optimized PCR reaction system for ISSR analysis was established:PCR was performed in a 20μl reaction mixture with 5μmol/L DNA, 2.5mmol/L Mg~(2+), 0.3mmol/Lof each dNTP, 300nmol/L primer, 1U Taq polymerase and 1×Buffer. Using 9 ISSRprimers,the genetic diversity among 23 Arrhenatherum elatius L.

3遗传多样性研究结果表明：通过模板DNA、引物、Mg~+、dNTPs、Taq酶五种ISSR反应体系的影响因素进行L_(16)(4~5)正交试验，建立了高燕麦草ISSR反应体系：20μl的反应体积中含有模板DNA 5μmol／L，引物300nmol／L，Mg~+2.5mmol／L，dNTP 0.3mmol／L，Taq酶1U及1×Buffer。

Ten assession of Castanopsis hystrix from different ecological areas was analyzed using the RAPD method,and 30 effective primers were selected from 100 random amplified primers.

采用从100个随机引物中筛选出的30个有效引物，利用RAPD技术对不同地区的10个不同生态型优质红锥种源进行遗传多样性研究。

The universal primers were designed according to the sequence of 16S rRNA, then 13 strains bacteroides and the other non-bacteroides were amplified by PCR.

以该属细菌的标准16S Rrna基因序列为靶基因设计通用引物，对该属标准菌株及不同来源的临床株进行PCR扩增，然后对扩增产物进行基因测序验证引物的特异性和敏感性。

(1)Both the liver regeneration and apoptosis are influenced by conspicuous bilirubinemia, hepatectomy and hepatic artery resection in rats after obstructive jaundice, especially by the liver resection volume and conspicuous bilirubinemia.(2) Under the condition of conspicuous bilirubinemia, the influence of 70% PH with HAR on liver regeneration and apoptosis is severer than its counterpart of 42% in the rats. Thus, the former is not safe and should be avoided and the later can be used.

高胆红素血症时，肝切除量是影响大鼠肝切除联合肝动脉切除实施安全性的重要因素，42%肝切除联合肝固有动脉切除、胆肠再通内引流，对肝细胞再生和凋亡影响较小，安全可行；70%肝切除联合肝动脉切除、胆肠再通内引流后肝细胞再生显著受抑制，凋亡增多，死亡率高，应避免实施。

In searching for RAPD marker linked to the genes controlling brachytic stem, 260 RAPD primers were applied to screen four parents of three combinations and RIL. Polymorphic bands revealed by the primer S-506 exhibited the best repeatability among all primers.

利用4个亲本和1个重组自交家系筛选260个RAPD随机引物，其中有1个引物S-506扩增出的多态性条带有较好的重复性。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐