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On the basis of gathering data from previous study and researching the stabilization and reinforcing technique of the typical high slope in west approach road, through in-situ tracking investigation and monitoring to the west road, and by way of studying the interaction between slope body and anchoring structure, monitoring data, the result of grey prediction and fuzzy comprehensive assessment, and adopting the idea of integrating prototype investigation and indoor experiment analysis, mechanism analysis and simulation research method, safety factor analysis and interacting relationship study, in-situ monitoring and safety evaluation, and using the theories of engineering geology, geotechnique, rock body mechanic, finite element method and fuzzy mathematics, this paper studies the long-term safety of the typical high slope specially.
本文在收集前人研究资料的基础上,采用原型调研与室内分析相结合、机制分析与模拟研究相结合、安全系数分析与相互作用关系研究相结合、现场监测与安全性评价相结合的思想,通过对鹧鸪山隧道西引道典型高边坡的现场跟踪调研与监测,运用工程地质、岩土工程、岩体力学、有限元理论、灰色系统理论、模糊数学等先进理论和方法,在对西引道典型高边坡的稳定性、整治与加固技术研究的基础上,通过边坡地质体与锚固结构相互作用分析、边坡监测数据分析、边坡变形灰预测分析以及模糊综合评判等途径,重点对治理后的典型高边坡的长期工程安全性进行了系统深入的研究。
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2 STR-PCR We analysed the results of STR with the silver dye in the beginning. The silver dye of STR analysis on a sporadic DMD family shows the band confirmation is subjective by eyeballing. It can not detect the small repeats and the size of polymorphic fragments. For limitation of the silver dye, we analysed the band size of STR with the SAGAGT Genotyping System. Primer are designed according to the nucleotide sequence of five STR markers (located in 5' terminus and intron 44, 45, 49, 50) of dystrophin gene. The 5' end of the forward primer are attached a M13 tailed primer which conjugated to the infrared flourescent dye.
根据dystrophin基因5个多态位点(44、45、49和50号内含子以及5'端启动子区的序列)设计引物,在这些STR的上游引物5'端加一可被红外线荧光物质IRDye700识别标记的M13fw的尾:CAC GACGTT GTA AAA CGA C 通过PCR扩增出重复序列本身,经高分辨率的变性聚丙烯酰胺凝胶电泳后,利用红外激光遗传分析系统及SAGA〓基因分型软件对凝胶上的扩增产物及内标的位置,进行扫描分析,确定产物片段的大小。
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Then we extend a lemma of Paley-Zygmund to-Zygmund to the cases of non-equally distributed and study the value distribution of more general random Dirichlet series with this lemma.
通常随机级数研究的是同分布随机变量序列,这里通过推广Paley-Zygmund引理,把随机级数的研究引向一般得多的不要求同分布的情况。
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Phylogenetic analysis revealed that there exists at least 2 genetical distinct hantaviruses lineages.
结果 选用50个随机引物进行扩增,有5个引物获得清晰、多态性高的RAPD谱带。
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The main results were as follows: The stable and high efficient AFLP analysis system of peach genom...
主要研究结果如下:建立了桃的稳定可靠的AFLP分析体系:高质量的基因组DNA是AFLP分析体系的关键步骤;E+0/M+0或E+0/M+C引物组合对预扩增条带数的影响不显著;选择性扩增引物组合E+2/M+3和E+3/M+3都可用于基因组的研究,但是前者的多态性高于后者;银染显色法具有成本低、检测时间短、避免同位素污染等优点,是一种安全、快速、有效的方法。
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The RAPD system of Gladiolus was optimized. Of the 12 random primers analyzed in this research, 9 primers can show steady and polymorphic amplification profiles.
建立了适用于唐菖蒲RAPD分子标记的反应体系,从12条随机引物中筛选出9条可扩增出多态性条带的引物。
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Methods The total RNA of leucocyte was extracted 43 allergic vasculitis patients and 24 normal subjects before and after treatment with Trizol reagent and the integrity and concentrations of HLA-DRw53 gene mRNA were determined by agarose gel electrophoresis and UV spectrophotometer after denatured with 1% formaldehyde, the product was transcribed into complementary retroviral synthetic deoxyribonucleic acid, and sequence-specific primers were designed based on the sequences of second HLA-DRw53 gene exon and housekeeping gene glyceraldehyde phosphate-dehydrogenase cDNA.
利用随机引物逆转录合成互补脱氧核糖核酸,根据HLA-DRw53基因第二外显子序列和管家基因人磷酸甘油醛脱氢酶的cDNA序列设计序列特异性引物。检测43例变应性血管炎患者治疗前后与24名正常人HLA-DRw53基因mRNA表达量的变化。
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N transfer Taocha Headwork water diversion project is run by the State Council approved the construction of the national large-scale water conservancy project is a project of N transfer an important part of both the water diversion project Headwork middle main channel Butterfly Valveswater diversion headworks but also Danjiang auxiliary dam reservoir engineering design for the new site redevelopment and build more power stations buildings are mainly cited sewers grvity induced gate stilling basin power plant and management of space and so on.
南水北调中线陶岔渠首枢纽工程是经国务院南水北调办批准建设的国度大型水利工程,是南水北调中线一期工程的重要状干部分,渠首枢纽工程既是南水北调中线输水总干渠的引水渠首,也是丹江水库的副坝,工程设计为新址重建,并增建发电站,建筑物主要有引渠、重力坝、引水闸、消力池、电站厂房和治理用房等。
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The amplified porducts of AY204223 exhibited polymorphism in wild swordtails, and displayed different band patterns among the three inbred Xiphophorus helleri.
结果 有4对引物可扩增出稳定条带,1对引物(AY204223)的PCR扩增条带在非选育群中表现多态性,在RR-B与BY-F系表型一致,与RW-H表型不同。
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Abstract] Objective To discuss the clinical analysis on 37 cases of hypertension intraventricular hemorrhage with soft channel technology-liquefied drainage of hematoma.Methods 37 hypertension brain hemorrhage patients,by the hematocele quantity,the shape and patient's condition were treated with soft channel technology-micro wound in the ventricles of the brain and haematoma liquefication drainage,to eliminate the hematocele,rescue the life,improve prognosis.
目的 探讨应用软通道技术-微创脑室内血肿液化引流术治疗高血压脑室出血的疗效方法对37例高血压脑室出血患者,依据脑室内积血的量、形态及患者的病情,行软通道技术-微创脑室内血肿液化引流术,清除血肿,抢救生命,改善预后。
- 推荐网络例句
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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.
随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。
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But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.
不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。
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Closes the eye, the deep breathing, all no longer are the dreams as if......
关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。