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Two 15nt complementary ssDNA primers were ligated into double stranded satRNA.Complete sequence of satRNA was obtained by RT-PCR using these 15nt ssDNA as amplification primers.Phylogenetic analysis showed that the identity of this 383nt satellite and some documented satRNAs was 72.6 to 99.5% at the nucleotide level.

以常规引物对感病植物的总RNA进行RT-PCR扩增，获得CMV RNA3全长克隆，经测序显示该RNA3序列属于CMV亚组Ⅱ；通过在卫星dsRNA两端分别加上15nt的单链DNA接头，以接头互补序列为引物进行扩增获得一个383nt的卫星RNA的全长序列。

The permanent magnet synchronous planetary transmission lift Dray Machine is dedicated to the elevator industry, dedicated to human Dray of the high-tech products.

而永磁同步行星传动电梯曳引机正是奉献给电梯产业，奉献给人类的的高科技的曳引机产品。

The power spectral analysis of electromyographic SEA may be helpful to characterize the sensitivity or intensity of MTrP, and more even to elucidate the pathophysiology of MPS.

引痛点自发性电位活动的平均积分面积及乙醯胆碱离子通道开启时间相当适合做为量化引痛点敏感度的参数。

It s difficult to discuss the existence of nontrivial solution for an elliptic equation involving critical exponent.

利用集中紧引理和Hardy不等式克服这个困难，运用山路引理证明了一类非线性椭圆方程非平凡解的存在性

For the homoclinics taking on values in the unbounded domain, in order to apply the usual "Mountain Pass " theorem, A compact embedding theorem is proved.

为克服同宿解取值于无界域而缺乏自然紧性，证明了一个紧嵌入定理，从而可以利用通常的山路引理与对称山路引理获得同宿解的存在性与多重性条件。

Methods The clinical data of 28 cases of reexpansion pulmonary edema from 1980 to 2006 were analyzed retrospectively.There were 11 cases after spontanecous pneumothorax drainage and operation, 9 cases after empyema drainage and operation ,5 cases after pulmonary operation and 3 cases after thoracic huge tube tumor resection.

回顾性分析1980～2006年12月28例复张性肺水肿的病例资料，其中自发性气胸引流和手术后发生11例，脓胸引流及手术后发生9例，肺部手术5例，胸腔巨大肿瘤切除术后发生3例。

In my experimentⅠcompare the purposes of different combinations of endoenzyme(MseⅠ-HindⅢ, MseⅠ-PstⅠ, MseⅠ-EcoRⅠ) and find that MseⅠ-HindⅢeasily finds thepolymorphism by primers combinations suiTab for AFLP analysis.And by means of this there aremany polymorphism strips. So the double endoenzyme of AFLP was exercisedfor the analysis of soybean cytoplasmic male sterility.In the cource of my experiment, 64 primerpairs of MseⅠand HindⅢwere exerted to analyse the twe mtDNA pools which are made ofsterilities and holdings and the polymorphisms of sterilities and holdings.

运用扩增片段长度多态性的方法，对独特的杂交大豆试材进行分析研究，通过比较MseⅠ-HindⅢ、MseⅠ-PstⅠ、MseⅠ-EcoRⅠ不同酶切组合的效果，结果表明MseⅠ-HindⅢ较容易找到多态性引物组合，而且具有较高的多态性，本试验选用MseⅠ-HindⅢ进行双酶切大豆线粒体DNA从而完成AFLP的分析工作，试验用64对MseⅠ和HindⅢ引物对两个不育系及保持系材料的线粒体DNA制成的pool进行AFLP标记分析，分析了不育系与保持系之间的多态性； 4。

A new type of extensometer for low temperature is designed and the working principle and technical charac-teristic of it is introduced,meanwhile,the force-displacement curve of a round-bar made of16MnR steel is tested at-196℃.

设计了一种新型低温引伸仪，介绍了其工作原理、技术特点，并采用此引伸仪测试了16MnR钢圆棒试样-196℃下的拉力-位移曲线。

And established the mechanics model,optimization parameter model,optimization mathematics model for the elastic element of the extensometer,programs analysis file and optim...

以应变计式引伸计弹性元件的结构优化为例，建立了引伸计弹性元件的力学模型、优化参数模型、优化数学模型，用ANSYS的参数化设计语言编制了分析文件和优化控制文件，经计算获得最优结果。

The one-side electronic extensometer fastened on only one side of tensile specimen cannot eliminate the effect of bend of eccentric tension. The results of measurement of such electronic extensometer are instable and then more errors may be included.

目前，国内外使用的电子引伸计多数是测量拉伸试样单侧的变形，由于拉伸试样往往发生偏心拉伸，单侧测量的引伸计无法扣除偏心拉伸的影响，因而其测量结果极不稳定和产生比较大的误差。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。