引

It is the first time that no additional anode was adopted in the magnetic macroparticles filter. At the same time the auto-re-igniting system was designed for vacuum cathodic arc deposition instrument. It ignites the arc with an electronic component with high voltage and can auto distinguish weather the arc dies out or is on during the deposition via a main power supply feedback unit. The energy for igniting the arc is very less and thus can successfully prevent the film being contaminated.

设计制造的电子式真空阴极弧离子镀引弧器成功地实现了高压脉冲引弧，可以多次重复使用，所用能量较小，可以有效防止对所镀膜的污染，引弧可靠，能够自动识别电弧是否引燃，能够在熄弧后自动重新引弧，重新引弧的速度快，有效防止了熄弧引起的气压波动，使镀膜过程中气压非常稳定。

The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result.

本发明提供了一种检测X、Y精子分离纯度的引物，该引物是针对牛Y染色体上性别决定基因Sry通过PCR错配技术设计而成，通过该引物可扩增片段大小为295bp，为了防止假阳性出现，本发明根据牛3号常染色体报道序列设计了一对内标引物C34，扩增片段大小为208bp，通过上述两对引物对单个牛精子进行双重PCR扩增，然后根据对检测结果的统计分析，对分离精子纯度做出最终评价。

Results 4 cases were treated by thoracic close drainage as long term treatment; 6 cases were cured with exclusive thoracic close drainage; 123 cases of bronchopleural fistula were treated with decortication or thoracoplasty after their symptoms of ardent fever, cough and expectoration vanished due to drainage ; 23 cases of hronchopleural fistula were treated with thoracic close drainage, cleaning up focus of infectionrestoring fistulae-splanchnic wall decortication and repeated chest drainage ,4 cases were cured and the extent of operation and thoracic deformity in 19 cases were diminished.

结果 4例不能耐受进一步手术者采用闭式引流术作为永久性的治疗；6例单纯引流治愈；123例因支气管胸膜瘘等经引流后高热、咳嗽、咳痰等症状消失，择期行胸膜纤维板剥脱术或胸廓成形术；23例合并支气管胸膜瘘拟行胸廓成形术的患者，经引流、病灶清除-瘘修补-脏层纤维板剥脱术、再引流，4例治愈，19例减小了手术范围及胸廓畸形的程度。

The results of measurement of such electronic extensometer are instable and then more errors may be included. The first writer of this paper designs the two-side electronic extensometer, which also has two edges but it can be fastened on two symmetric sides of the tensile specimen.

最近四十年发展起来的各种电子引伸计在讯号放大及数据采集和处理方面有非常突出的优点，但大多数电子引伸计如单侧电子引伸计(图1)、光栅引伸计和视频引伸计都是测量拉伸试样单侧的变形[3]，笔者称它们为单侧电子引伸计。

C Supports both Firmware Hub and LPC Memory Read and Write Cycles Auto-detection of FWH and LPC Memory Cycles C Can Be Used as FWH for Intel 8xx, E7xxx, and E8xxx Series Chipsets C Can Be Used as LPC Flash for Non-Intel Chipsets Flexible, Optimized Sectoring for BIOS Applications C 16-Kbyte Top Boot Sector, Two 8-Kbyte Sectors, One 32-Kbyte Sector, Three 64-Kbyte Sectors C Or Memory Array Can Be Divided Into Four Uniform 64-Kbyte Sectors for Erasing Two Configurable Interfaces C FWH/LPC Interface for In-System Operation C Address/Address Multiplexed Interface for Programming during Manufacturing FWH/LPC Interface C Operates with the 33 MHz PCI Bus Clock C 5-signal Communication Interface Supporting Byte Reads and Writes C Two Hardware Write Protect Pins: TBL for Top Boot Sector and WP for All Other Sectors C Five General-purpose Input Pins for System Design Flexibility C Identification Pins for Multiple Device Selection C Sector Locking Registers for Individual Sector Read and Write Protection A/A Mux Interface C 11-pin Multiplexed Address and 8-pin Data Interface C Facilitates Fast In-System or Out-of-System Programming Single Voltage Operation C 3.0V to 3.6V Supply Voltage for Read and Write Operations Industry-Standard Package Options C 32-lead PLCC C 40-lead TSOP

0第0页，本页显示记录0-0，共0条记录分0页显示C支持两种固件中心和LPC内存读取和写入周期自动的FWH和LPC的记忆圈C检测可以用于英特尔8xx系列，E7xxx，E8xxx系列芯片组和C可以用作FWH与至于非英特尔芯片组的BIOS应用柔性优化扇区开放16字节热门引导扇区，两个8 - Kbyte的，一个32字节部门，3个64 - Kbyte的C或存储阵列，线性预测编码闪光可分为四个统一为两个可配置的接口擦除的FWH / LPC接口为64 - Kbyte的行业，系统运行C地址/地址多路复用在制造过程中用于编程接口的FWH /线性预测编码界面C与33 MHz的PCI总线时钟 5信号通信接口进行操作，支持字节读取和写入引导扇区的顶部和WP C两硬件写保护引脚：任务型为所有其他部门 5个通用输入的系统设计的灵活性识别的多种设备选型部门登记销锁定为个别部门读取和写保护的A /阿复用界面C 11引脚复用引脚地址和8引脚的数据界面C促进快速系统内或外的系统编程的单电压3.0V至3.6V的操作供应的读取和写入操作业界标准的封装选项电压 32 -引脚PLCC 40引脚的TSOP

The incidence rates of wound infection and ankylenteron and hospital durations in the two groups were observed and compared with each other.

结果引流组与非引流组切口感染率分别为19.0％和8.0％（P〈0.05）；肠粘连发生率引流组与非引流组分别为10.7％和4.5％（P〈0.05）；腹、盆腔残余脓肿的发生率分别为1.1％和1.2％（P〉0.05）；引流组与非引流组病人术后住院天数分别为（9.3 2.7）d和（5.1 1.9）d（P〈0.05），引流组还存在拔管恐惧、脱管等并发症，结论穿孔性阑尾炎术后不放置腹腔引流更明显地降低术后并发症，且明显缩短住院天数。

Fragments from the 239th to the 944th nucleotide of the coding regions of all actin mRNAs were amplified with two actin specific primers. Fragments from the 239th nucleotide of the coding region to the end of actin mRNAs were also amplified The electrophoresis results were compared between the macropterous and brachypterous brown planthoppers.

利用两条肌动蛋白特异性引物，扩增出了肌动蛋白基因mRNA的编码区片段，另外，将肌动蛋白特异性引物作为上游引物，将与3'锚定反转录引物部分序列相同的引物作为下游引物，扩增出了从肌动蛋白基因mRNA的编码区第239号碱基到Poly尾的这段cDNA，并在翅型之间作了比较。

Early racket preparation 早些引拍 One of the reasons the pros do this is because it is more natural to run to meet the ball with the racket in front of them rather then behind.

专业选手这样做的原因之一是他们的引拍到位的点会靠前，这样做比靠后的点在迎击球时更加自然。

In despite of long primer PCR reaction performed under low stringency or high stringency or by two steps, the assay showed that the PCR products which were clear and bright did not include the target sequence.

因此，本实验中的长引物(19、22碱基)不适合单引物PCR；在较短的引物中，6、8碱基引物不适用于单引物PCR，而10碱基单引物PCR则是一种可行性较高的染色体步移策略。

The method is just like the QuikChange site-directed mutagenesis method except for its special primer. This pair of primers is composed of a long primer and a short primer which is central overlapped.Both of the Tm of 5\'sequence in long primer and Tm of 3\'sequence in short primer are above 66℃.The Tm of short primer is almost half of that of long primer.It is convenient to perform insertion or substitution mutation of DNA fragments larger than 20bp,In addition,it is highly applicable in research about the deletion mutation of DNA fragments larger than 2000bp.

我们实验室一直在寻找一种简单高效的可以用于基因的删除、插入及替代突变的方法，通过大量研究和实验发现，可以使用一种基于反向PCR原理，使用全新的引物设计的突变方法——&COP&突变法，该方法采用与QuikChange点突变法相同的操作步骤，其策略是设计一对中心重合的引物，长引物的5\'端序列和3\'端序列的Tm值均不低于66℃，短引物Tm值约为长引物一半，且与长引物中心重合互补。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失