弓形的

The interconversion between tachyzoite and bradyzoite is the key of pathogenesis of toxoplasmosis.

弓形虫速殖子与缓殖子的相互转化，是弓形虫致病的中心环节。

Objective To evaluate the potential application of the recombinant multiple epitope antigen in diagnosis of toxoplasmosis.

目的 评价弓形虫多表位基因重组抗原在弓形虫感染诊断中的效果，探索多表位抗原在弓形虫免疫检测中的应用前景。

objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

作者单位：山西医科大学寄生虫教研室，太原 030001；化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，将家蝇幼虫抗菌肽进行分离纯化，采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后，筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽，而且不止一种，但其效果有所不同。

Toxoplasmosis is zoonosis. The effective integrated control measure is to change people's bad habit and arouse their safety consciousness.

结论弓形虫是人畜共患病，应采取有效的综合防制措施，加强宣传，改变人们的不良习惯，提高个人防护意识，可降低人群弓形虫感染。

The decidual NK cells were collected and cell subset change of the two groups was analyzed by flow cytometry.

目的通过检测弓形虫感染后早孕蜕膜自然杀伤细胞亚群的变化，探讨其与弓形虫感染致不良妊娠结局的相关性。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

Results In 30 sera with anti-Toxoplasma IgM antibodies and 28 with IgG antibodies, the sensitivity for IgM and IgG antibody detection was 90.0%（27/30） and 85.7%（24/28） respectively, the specificity was all 100% in examining 40 healthy control sera, and the Youden index was 0.9 and 0.86 respectively.

结果 用免疫印迹试剂盒检测30份弓形虫IgM和28份IgG阳性血清，敏感性分别为90.0%（27/30）和85.7%（24/28），40份健康对照者血清的弓形虫IgM和IgG抗体均为阴性，特异性均为100%，Youden指数分别为0.9和0.86。

It is found that for the same volumetric flow rate, the pressure drop in the shell side of the curved baffle heat exchanger is remarkably lower than that of the traditional one. And the pressure drop percentage deceases even more for a smaller curvature radius of the curved baffle and height of the baffle cut.

结果发现：在流量一定的情况下，曲面弓形折流板换热器的壳程压力降明显低于普通弓形折流板换热器的壳程压力降，而且随着曲面折流板曲率半径和折流板圆缺口高度的减小，曲面弓形折流板换热器的壳程压力降降低的百分比增大。

The distance difference between 6/8/6/4 taxoids and 5/7/6/4 taxoids indicates that the role of oxetane is to form a suitable bow-shaped skeleton with 6/8/6 tricycle.

环氧丙烷结构类型化合物弓形口间距值明显区别于其它三类化合物；5/7/6/4与6/8/6/4类型化合物弓形口距离不同，说明紫杉烷二萜化合物的环氧丙烷结构对活性的作用是和6/8/6三环骨架共同支撑一个合适大小的弓形架。

One is preshaped NiTi rocking-chair archwire whose form is like letter "L", the other is the standard arch form rocking-chair archwire which is shaped by orthodontists in clinic according to various standard arch forms of straight wire appliance.

临床常用的摇椅弓形主要有两种：一种是&L&形的预成NiTi摇椅弓；另一种是我们在临床上自己弯制的、与各种标准弓形一致的标准弓形摇椅弓。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。