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To evaluate the specificity of the PCR, genomic DNA of Theileria annulata,Babesia bovis,Toxoplasma gondii,Leishmania donovani and standard strain of N. caninum were used as a template in the PCR. For determining the detection limit of amplification procedure, PCR was run on a dilution series of genomic DNA from N. caninum(1.562 5-200 ng/ml). Brain tissue samples of 32 aborted fetuses were detected by PCR-based assay, and 23 blood samples from mothers were tested by ELISA. Results The amplified DNA fragment (350 bp)had a high identity of 98% with the Nc-5 gene sequence of N.

以环形泰勒虫、牛巴贝斯虫、刚地弓形虫、杜氏利什曼原虫以及犬新孢子虫标准株DNA为模板进行扩增以验证PCR的特异性,采用紫外分光光度计测定犬新孢子虫标准株DNA浓度和纯度,取高纯度的DNA样品用灭菌水稀释,分别取不同量的DNA进行PCR扩增,确定PCR方法的敏感性;利用该方法对32份奶牛流产的胎牛脑组织样品进行检测,同时,对其中23份流产的母牛血样进行ELISA血清学检测,以评价犬新孢子虫PCR方法的检测效果。

Through Liaoning Province Taian bills farmers first 35 suspected cases of classical swine fever diagnostic, recorded incidence of death from the process and will have a record of 11 symptoms, and so Autopsy examination step analysis, and later in the Shenyang Agricultural University Veterinary College pastoral guidance from the laboratory diagnosis, through production observation, classical swine fever virus onto the world body immune reaction, Enzyme linked immunosorbent assay testing, and made with Erysipelas ,Swine plague, Streptococcosis suis , and Mycoplasmal pneumomia of swine, Swine Toxoplasmosis , and ultimately determine the reasons for the cause of classical swine fever virus incidence.

本人通过对辽宁省台安县某农户的35头猪瘟疑似病例进行诊断,记录了从发病到死亡的过程,并且将所出现的症状一一记录,对死亡的猪挑选了3头进行剖检做近一步分析,对疑似病症进行分析排除。之后提取病料并保存,在沈阳农业大学畜牧兽医学院赵玉军教授的指导下进行了实验室诊断,通过涂片观察、猪瘟病毒兔体交互免疫反应、酶联免疫吸附实验,并做出了与猪丹毒、猪肺疫、猪气喘病、猪链球菌、弓形虫病的鉴别诊断,最终确定为此次引起发病的原因为猪瘟病毒。

The raw data was processed by using axial, coronal and double oblique multi-planar reformation; Images obtained were graded in terms of quality with a 5-point scale(5=excellent, 4=good, 3=fair, 2=poor, and 1=nondiagnostic). In grading image quality at axial MPR, The radiologic specialists focused on the subarcuate fossa, tendon of tensor tympani, facial recess, vestibular aqueduct and pyramidal eminence, In grading image quality at coronal MPR, attention was given to the scute, crista transversa, fenestra cochleae, lateral malleal ligament and snake eyes signature of cranial nerve, In the grading of the image quality at double oblique MPR, the radiologic specialists concentrated on the malleus, incus, stirrup bone, upper bony semicircular and aquaeductus fallopii, and then to implement statistical analysis. In order to choose the minimum tube current values and the maximum pitch that can satisfy the diagnosis request, image quality of axial, coronal and double oblique reformation images was compared with different tube current groups. 15 ears of volunteers were used to test the validity with the scanning parameter. Subsequently noise, MTF and dose length product were measured by phantoms in different tube current and pitch, the parameters obtained were compared and taken into statistics analysis.

扫描模式使用临床常用的颞骨螺旋扫描方式:管电压120 kV,准直宽度20×0.6 mm,视野200 mm,重建矩阵512×512,旋转时间1 s/r,重建层厚0.6 mm,重建间隔0.3 mm,分别改变管电流(380、300、200、160、120和80 mA)和螺距(0.8、1.0和1.2)进行扫描和重建,然后对颞骨进行横断面、冠状面和双斜面多平面重组,于重组后的横断层面图像上选取弓形下窝、鼓膜张肌腱、面神经隐窝、前庭导水管和锥隆起5个解剖结构,冠状面重组图像上选取盾板、横嵴、蜗窗、面神经的蛇眼征和锤骨外侧韧带5个解剖结构,双斜面重组图像上选取锤骨、砧骨、镫骨、上骨半规管和面神经管5个解剖结构,在双盲的情况下由放射学专家分别对各管电流和螺距下扫描的重组图像进行评分,随后进行统计学处理,从中筛选出满足诊断要求的最低管电流值和最大的螺距,分别采用患者25例(15耳)用该管电流值和螺距验证其可行性;然后利用模体分别测试各管电流和螺距下的图像的空间分辨率、噪声及其剂量长度积,并对测试所得参数数值进行比较和统计学处理。

Now Im complete, uh huh Still stallion, brick house, pile it on Ryde or Die, bitch, double R, cant crawl Beware, cuz I crush anything I land on Me here, aint no mistake nigga it was planned on And if I had to give you up Its only been a year Now I got my foot through the door And I aint goin nowhere It took awhile to get me in And Im gonna take my time Dont fight that good shit in your ear Now let me blow ya mind by lune

现在 Im 完成, uh 哼!使种马,砖块房子,堆起它安静在 Ryde 或一钢模,母狗,两倍的 R,伪善之言爬行小心, cuz 我粉碎我登陆的任何事在我在这里, aint 没有错误 nigga 它被计画在而且如果我必须放弃你它的唯一是一年现在我使我的脚通过门而且我无处的 aint goin 它一会儿拿让我获得录取而且 Im 去从容不迫 Dont 在你的耳朵中对抗好粪现在让我吹 ya 思想藉着弓形

In the chapter 5, during the optimization, the influence of search efficiency of choosing search variable is studied, and author proposes a kind of choice, which the positions of two exit points and the sagitta of the slip surface are taken as the search variable (the sagitta depends on the dynamic positions of two exit points of the slip surface), The examples show that the choice method decrease search region, improve the efficiency of searching, and has applying foreground.

在第五章中,探讨了搜索变量的选取对搜索效率的影响,对此作者提出以滑弧的两个端点位置以及它所组成的弓形高为搜索变量。实例表明,搜索变量的这种选取方法缩小了搜索范围,提高了搜索效率,有很好的应用前景。

The trees had a light delicate foliage, the structure of which was different from that of the trees in Europe; it did not grow in bows or cupolas, but in horizontal layers, and the formation gave to the tall solitary trees a likeness to the palms, or a heroic and romantic air like full rigged ships with their sails clewed up, and to the edge of a wood a strange appearance as if the whole wood were faintly vibrating.

树木都长着轻巧而细致的枝叶,它们的构造和欧洲树木的结构不同;树冠并非长成弓形或者球型,而是一层一层的生长,让树看起来就像一个个手掌,或者有一种类似升起风帆整装待发的帆船的豪气而浪漫的感觉,森林的边缘也有一种怪异的样子,似乎整个森林都在轻轻摇摆。

The trees had a light delicate foliage, the structure of which was different from that of the trees in Europe; it did not grow in bows or cupolas, but in horizontal layers, and the formation gave to the tall solitary trees a likeness to the palms, or a heroic and romantic air like fullrigged ships with their sails clewed up, and to the edge of a wood a strange appearance as if the whole wood were faintly vibrating.

树上只有轻轻薄薄纤细的树叶,树冠的结构不同于欧洲的树,既非弓形也不似穹顶,而是水平的层层分布的,这个形状让高且孤的树有点像棕榈树,也赋予了它好似收拢风帆的全帆缆船般的英雄色彩和浪漫气息,树的边缘的样子有些奇怪,好像整棵树都在微微颤动。

The famously imposing Arc de Triomphe memorializes military victory and marks the crossroads of 12 streets.

意译:巴黎著名的壮丽的弓形门是当年民众请愿和军事的斗争的胜利,位于第十二街的十字路口。

Instep part of closely integrated with the shoes, it can firmly support the body's generous heel; shoe heel height of 2-3 cm, with anti-skid pattern on the soles, the soles of the feet correctly to maintain the bow-shaped curve, high heels, easy to fall off the sandals, high-heeled clogs, etc. are not suitable for the easily falls.

脚背部分能与鞋子紧密结合,有能牢牢支撑身体的宽大的后跟;鞋后跟的高度在2—3厘米左右,鞋底上带有防滑纹,能正确保持脚底的弓形曲线,高跟鞋、易脱落的凉鞋、高跟木屐等容易跌跤的都不适宜。

Included among them are: characteristic frequency curve of reinforced medium; critical length of the anchor bar ; anchor bar density and nonuniform coefficient of the reinforced medium; embedded anchorage layer effect; bed plate effect; weak surface effect; distortion of the bow-shaped distributed state of the anchor bar frictional resistance and initiation, formation and development of the dominant glide control plane; criteria about the displacement velocity of side-wall stability status, critical status and unstability status, and the system of ordinate analysis method, et al

d。文中提出的若干新的概念、判据和分析方法,如加固介质特征频度线;临界锚杆长度;锚杆密度及加固介质不均匀系数;嵌固层效应;垫板效应;弱面效应;锚杆&弓形&受力形态的畸变与优势滑移控制面的产生、形成和发展;边壁稳定状态、临界状态和失稳状态的位移速度判据;纵坐标系分析法等,具有一定的理论价值,并对软土工程围护设计具有指导意义。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。