异色的

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

METHODS: The models of abdominal aorta transplantation were made with micro-surgery in rats. The recipients were divided into three groups: allograft control group, atorvastatin-treated group and isograft control group. Vascular intimal thickness in all of the groups was observed by histological examination. The expression of proliferation cell nuclear antigenl and α-smooth muscle actin were determined by immunohistochemistry. The content of nitric oxide was measured by nitrate reductase chromatometry.

建立大鼠腹主动脉移植慢性排斥反应模型，分为3组：异系移植对照组、异系移植治疗组和同系移植组，60d后对移植动脉行病理组织学检测，观察移植动脉内膜增生程度；并行免疫组织化学染色，检测移植动脉增殖细胞核抗原和平滑肌肌动蛋白的表达情况，行硝酸还原酶比色法测定移植动脉组织NO的含量。

The results showed that the four stereoisomers of soman were well separated under the defined chromatographic conditions. CP-）soman was only detected stereoisomer in the intoxicated rabbit blood and eliminated rapidly, the toxicokinetics could be described by a two-compartment model after rabbits received soman via vein.

结果可见梭曼4个异构体在确定的气相色谱条件下得到了较好地分离；给兔iv梭曼43.2 μg·kg-1后，血中仅测到一对CP梭曼，其消除过程符合二室开放模型， V d=2.1 L·kg-1， t 1/2α=4.5 s， t 1/2β=72 s， AUC(15～300 s)=2.08 mg·s·L-1， CL =21 mL·kg-1·s-1，血中CP梭曼在血中消除过程反映了机体对梭曼毒性异构体的解毒过程。

A method for the determination of 12 sulfonamides (sulfanilamide, sulfamonomethoxine, sulfacetamide, sulfamethoxazole, sulfadiazine, sulfisoxazole, sulfathiazole, sulfadimethoxine, sulfamerazine, sulfaquinoxaline, sulfamethazine, sulfanitran) in cosmetics was developed by ultra performance liquid chromatography with photodiode array detector. The chromatographic column used was Acquity UPLCTM BEHC C18 (50 mm×2.1 mm, 1.7 μm) and the mobile phase was acetonitrile-0.1% formic acid aqueous solution.

建立了采用超高效液相色谱-二极管阵列检测器测定化妆品中12种常见的磺胺抗生素(磺胺，磺胺间甲氧嘧啶，磺胺醋酰，磺胺甲基异唑，磺胺嘧啶，磺胺二甲异唑，磺胺噻唑，磺胺二甲氧嘧啶，磺胺甲基嘧啶，磺胺喹啉，磺胺二甲嘧啶，磺胺硝苯)的方法。

The separation of optical isomers of 2, 2'-dihydroxy-1, 1'-binaphthyl and 2, 2'-diamino-1, 1'-binaphthyl was studied by reversed-phase high performance liquid chromatography on a chiral stationary phase consisting of-hydroxypropyl-β-cyclodextrin (Cyclobond Ⅰ 2000 SP)using methanol/water as the mobile phase. The effects of mobile phase ratio, flow rate and column temperature on separation of optical isomers of DHBN and DABN were investigated.

在S-羟丙基－β-环糊精手性柱(CyclobondⅠ2000 SP)上用反相高效液相色谱法研究了2，2'-二氨基－1，1'-联萘和2，2'-二羟基－1，1'-联萘光学异构体的分离，考察了流动相组成、流速以及柱温等对联二萘胺和联二萘酚光学异构体分离的影响。

Borneol and isoborneol, the effective constituent in Chinese Medicine Shuxinping are quantitatively analyzed by GC and GC-MS with hexahydrothymol as an internal standard.

用气相色谱法和气相色谱-质谱联用法对舒心平的有效成分龙脑及异龙脑的提取液进行测定 [1] 。

Three flavonoids compounds: kaempferol *!, quercetin *, and isoquercitrin *# were isolated from the leaves of Cyclocarya paliurus Batal.

采用溶剂萃取及多种色谱技术分离纯化，根据理化性质和光谱分析方法鉴定其结构，确定从青钱柳中分离得到山柰酚、槲皮素和异槲皮苷 3个黄酮单体化合物，并用反相高效液相色谱法对其含量进行了测定，测得各自在甲醇和水提取物中的含量：山柰酚 0 。

A reversed-phase high performance liquid chromatographic method was established for determination of isothiocyanates in human urine , which used the reaction of propyl isothiocyanate with 1,2-benzenedithiol as reference instead of the synthesis of 1,3-benzodithiole-2-thione.

省去合成1，3 苯二硫杂环五烯 2 硫酮这一步骤，直接用异硫氰酸丙基酯与1，2 苯二硫酚反应作标准，建立了尿液中异硫氰酸酯的反相高效液相色谱测定方法。

Isopentanol, nitrochlorobenzene, nitrotoluene and diethylbenzene were well separated in the range of optimal carrier gas velocity of 13~17cm/s.

SE-30系列毛细管色谱柱的最佳载气线这范因为13~17cm/s，且在最佳线速度下很好地分离了异戊醇、硝基氯苯、硝基甲苯及二乙苯同分异构体体系。

Objective:An ionpair RPHPLC method was established to determine the amounts of lotusine, liensinine, isoliensinine and neferine in Plumula Nelumbinisimultaneously.

目的：建立同时测定莲子心中四种异喹啉生物碱莲心季铵碱、莲心碱、异莲心碱、甲基莲心碱含量的离子对反相高效液相色谱法。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。