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That PI and the percentage of the cells in s-phase of the borderline/malignant and heteroploid/aneuploid tumors were higher ted to higher mortality and relapse rate as well as the corresponding risk. Because of the changable characters and dissociative phenomenon of the tumor, the measurement of PI and the percentage of cells in s-phase may be disturbed in few of diploid tumor and in part of double DNA stem ones. Therefore other paramet- ers should be added to analyse before making any conclu- sion.

交界性和恶性、异倍体或非整倍体肿瘤的PI和S期细胞百分率较高,病死加复发率和相对危险性增加;由于肿瘤的异质性和"分离现象",对于少数2倍体肿瘤和部分双DNA干系肿瘤PI和S期细胞百分率的测定易受干扰,故应结合其它参数综合分析。

VCM increased the HRPE cells' proportion of phase S (6%~13%)and heteroploid was found in VCM with 250 and 500 mL/L vitreous.

VCM促使更多的HRPE细胞进入了DNA合成期(S期,6%~13%),含250与500mL/L玻璃体体积的VCM使HRPE细胞出现了异倍体的改变。

The gene regulating sequence of human collagen type Ⅰ is located at 5'end and first intron. The carriers of reporting gene was made, and the function of promoter, enhancer and inhibiter was examined in this study. On the basis of above work, MBP ectopic expression carriers were further built. The result laid a foundation for investigating the feasibility, and validity to specially express MBP gene of human nervous system, in tendon fibroblast.

人Ⅰ型胶原基因调节序列位于5'端和第一内含子,本研究构建了带报告基因的载体并检测了其启动子,增强子和减弱子的功能,以此为基础,进一步构建了MBP异位表达载体,为探索在神经系统少突胶质细胞特异表达的人脑髓鞘碱性蛋白MBP基因转至肌腱成纤维细胞表达的可行性和有效性奠定了基础。

The whole cell-patch clamp recording technique was used to record I in specific pipette solution and superfusion according to specific holding potential and command potential program.

采用经典的酶分离心肌细胞方法,应用全细胞膜片钳技术记录I,观察MI后I、的变化,予以β2受体激动剂salbutamol、β受体激动剂异丙肾上腺素及不同选择性β受体阻滞剂后I的变化。

Here we summarize the progress of salmon calcitonin's heterogenous expression in prokaryotic and eukaryotic expression systems as well as the amidation of C-terminal. Both the biological activity of salmon calcitonin and the biologic expression system can be improved by genetic engineering techniques.

本文综述了鲑鱼降钙素基因在大肠杆菌、链霉菌、乳酸杆菌和蓝藻等原核细胞,在酵母、昆虫、动植物等真核细胞中异源表达的研究进展,以及通过基因工程的方法能够改进生物表达系统,提高鲑鱼降钙素活性的动态。

Positive rate was relatively high, and the foreign gene could also maintain in testicular tissue for a long time, it could still be detected in the tissue in 7 months after being injected. However. Deferent results could be gained when the detection were made with different primers on different sites of the recombinant plasmid. This could be explained by transgene lost when the foreign gene recombinated and integrated in heterogenetic cell, or transgene degradated by cell nucleases.

然而,我们也看到,在后代检测中,利用不同的引物扩增重组质粒不同部位时,检测结果存在差异,转基因阳性率不同,这种差异说明当外源基因进入到异源细胞内,在重组整合时发生了外源基因丢失的现象,并由此导致外源基因的整合率下降,也可能是受细胞内核酸酶的作用,使得外源基因遭到降解。

The new method has better resolution and can be applied to larger systems than homonuclear NMR methods. Most proteins playing an important role in cells have to be studied with heteronuclear labeled samples.

异核方法的优势在于它的分辨率更好,可以研究相对较大的,同核方法研究不了的分子;对大部分在细胞中发现的意义重大的蛋白的NMR研究都必须采用异核方法。

The leaf in the Karst area was isolateral and had closely arranged mesophyllous cells.

在显微结构方面:岩溶区生长的扇叶铁线蕨的叶片具有旱生植物叶片特点,即叶片为等面叶,叶肉细胞排列较为紧密以及叶片维管组织发达等:而非岩溶区生长的扇叶铁线蕨的叶片为异面叶,特点为叶肉细胞排列相对疏松,维管组织不发达。

Methods: Eleven B-CLL patients were studied. Leukemic lymphocytes with (n=8) or without (n=3) P2z receptors were exposed in vitro to ATP, benzoylbenzoic-ATP, 2-methylthio-ATP(2MeSATP), adenosine-5′-[γ-thio] triphosphate, and other nucleosides for 8h. Apoptosis was detected by electron microscopy, agarose gel electrophoresis, and quantitative TdT assay. Results:Apoptosis was detected only in leukemic lymphocytes with P2z receptors. By using the quantitative assay, ATP-inducing DNA strand breaks were found to occur specifically for BzATP, ATP and 2MeSATP, but not for ATP-γS and other nucleosides.

将表达P2z受体[P2z]与不含P2z受体[P2z]的两组CLL细胞分别同1.0mmol/L三磷酸腺苷体外培养8小时,以电镜、DNA凝胶电泳和定量DNA 3′端TdT法检测细胞凋亡;并对ATP、苯甲酰苯甲酸ATP、2-甲基硫ATP(2MeSATP)、γ-硫代ATP及其它核苷的诱导效应和氧化型ATP、1-[N,O-二(5-异喹啉碘酰基)N-甲基-L-酪氨酰]-4-苯哌嗪KN-62)的抑制效应做定量研究。

Propofol can effectively reduce edema and cell apoptosis and play a protective role on cerebral hemorrhage indued by collagenase.

异丙酚可以有效减轻ICH后的细胞凋亡,并减轻细胞超微结构的改变,对胶原酶所引起的大鼠ICH具有保护作用。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

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