异细胞的

In part of edible fungi, two different nuclei in heterokaryon are generally consided to distribute evenly to all of colony through clamp connection, and the ratio of two nuclei should be 1:1. But other hypothesis was demurrable according aberrancy of nucleus ratio during monokaryotization of oidiospore and protoplast in F. velutipes. My experiment was constituted of two parts.

在部分食用菌中，一般认为异核体菌丝通过锁状联合这一细胞结构将两种核均匀分布到全部菌落，异核体中两种核的数量比例应该是1：1的关系；但金针菇原生质体单核化和粉孢子单核化过程中发生的核偏离的研究表明，异核体菌丝两种核的数量比例可能不是1：1的关系。

F1 hybrid has the morphological feature which is between the male and female parents and shoed strong heterosis, exuberant vitality, late ripe period and low rate of self-fertility. The average chromosome configuration of F1 in PMC MI was 18.58Ⅰ+18.59Ⅱ+0.085Ⅲ+0.028Ⅳ. At anphase Ⅰ in PMC, some laggard chromosomes arranged in equatorial plate. Micronucleus and a lot of abnormal cells can be observed at dyad, tetrad and microspore.

杂种F1形态特征介于双亲之间，具有较强的杂种优势，生长旺盛，熟期晚，自交结实率较低。F1花粉母细胞减数分裂中期Ⅰ平均染色体构型为18.58Ⅰ+18.59Ⅱ+0.085Ⅲ+0.028Ⅳ，PMC后期Ⅰ观察到部分落后染色体排列在赤道板上；二分体、四分体和小孢子时期，细胞内普遍观察到微核，且出现大量异形细胞。

Studies also indicated that genistein exsists not only in soybeans but also in many kinds of plants such as vegetables and fruits.As a potential anticancer agent, the actional mechanisms of genistein mainly includes as follows:First, genistein can depress the activity of protein tyrosine kinase and transduction pathways for the phosphorylation of receptors and mitosis signal. So genistein can lead to cells' proliferation depressed. Second, genistein has minimal effects of phytoestrogens. It can be combined with estrogen receptor and improve the synthesis of cellular sex hormone binding glulobin, and improve the activity of UDP-glucuronyl transferase. Through these pathways, it can inhibit the cell activity of breast cancer and prostate cancer.

作为一个很有潜力的抗肿瘤物质，三羟异黄酮的作用机制主要包括：①抑制蛋白酪氨酸激酶活性，可阻抑PTK引起的受体磷酸化和有丝分裂的信号传递，导致癌细胞增殖受抑；②弱雌激素作用：可通过与体内雌激素受体结合，并可增加细胞内性激素结合球蛋白的合成，增加UDP-葡糖醛酸转移酶的活性等途径抑制乳腺癌和前列腺癌细胞活性；③拓扑酶Ⅰ和Ⅱ抑制剂，抑制细胞活性；④上调细胞周期性负性调节因子P21WAF1/CIP1的表达，使之负性调节因子作用增强；⑤可阻止胰岛素样生长因子-Ⅰ、肝细胞生长因子和神经生长因子的作用而抑制肿瘤生长；⑥其他：抗氧化作用、抑制热休克蛋白、诱导细胞凋亡、抑制新生血管生成和抑制多种耐药相关蛋白等。

Part one Isolating, culturing, chondroblast induction and evaluation of human MSC in vitroObjective:To examine the property and proliferation ability of primary culturing MSC ,mvestgate the effect of lightly centrifugation and chemical definition medium to induce MSC differentiate into chondroblast.

结果：原代骨髓基质干细胞易于体外培养，增殖旺盛；经轻度离心和化学限定培养基诱导后细胞呈高密度生长，仍保持较旺盛的增殖能力，细胞转化成圆形肥大细胞，甲苯胺蓝染色显示软骨细胞外基质 v的特异性异染，11胶原蛋白免疫细胞化学染色呈强阳性，BT干CR鉴定显示＊胶原mRNA丰富的表达。

In the BC〓F〓 and BC〓F〓 generation of Yannong15×Thinopyron intermedium hybrid, 4 octoploids which had chromosome number of 2n=56 and could form 28 bivalent at PMC MI, 14 disomic addition lines which had chromosome number of 2n=44 and could form 22 bivalent at PMC MI; three monosomic addition lines which had chromosome number 2n=43 and had the chromosome configuration of 21Ⅱ+1Ⅰ at PMC MI, 1 substitution line which had chromosome number 2n=42 and could form 21 bivalent at PMC MI were selected by observation of mitosis in root tip cell and meiosis in pollen mother cell.

在烟农15与中间偃麦草杂交的BC〓F〓和BC〓F〓代中，通过根尖有丝分裂和花粉母细胞减数分裂中期Ⅰ观察选出4个2n=56，PMC MI染色体构型为28Ⅱ的八倍体小偃麦；14个2n=44，PMC MI染色体构型为22Ⅱ的双体异附加系；3个2n=43，PMC MI染色体构型为21Ⅱ+1Ⅰ的单体异附加系；1个2n=42，PMC MI染色体构型为21Ⅱ的异代换系。

In present study, we demonstrated that μ opioid receptor and D1 dopamine receptor could form heteromeric complexes in membranes preparation from HEK293T cells transiently cotransfected with both receptors and from mice striatum.

在本工作中，我们通过免疫共沉淀和BRET法证明，HEK293T细胞上瞬时表达的μ阿片受体和D1多巴胺受体能形成异源二聚体，并证实μ阿片受体和D1多巴胺受体异源二聚体存在于小鼠纹状体中。

Then transplantation of the sciatic nerve from donor rats to the recipient rats in two groups were progressed.7 days after nerve transplantation,the contents of cd4 and cd8 in peripheral plasma of recipient rats were detected by flow cytometry.8 weeks after nerve transplantation,transplanted nerve were observed under light microscope.results:compared with nonirradiated group,the percentage of cd4 and the ratio of cd4 to cd8 in uvb irradiated group were significantly decreased（p＜0.05,p＜0.01）,cd8 percentage was significantly increased（p＜0.01）.conclusion:uvb-irradiated donor splenocytes were injected in the recipient rats through caudal veil,which could induce immunological tolerance to allogeneic transplantation.

未照射组注射未经紫外线照射的供体脾细胞，照射组注射经紫外线照射的脾细胞，7 d后移植供体神经。术后7 d行外周血t淋巴细胞亚群检查，术后8 w行移植神经组织学检查。结果：照射组cd4+百分率、cd4+/cd8+比值较非照射组明显降低（p.01，p.05），cd8+百分率较非照射组明显升高（p.01）。组织学检查显示，照射组的再生神经较非照射组多。结论：尾静脉内注射经紫外线照射的异基因脾细胞可诱导对异体神经移植的特异性免疫耐受。紫外线；脾细胞；免疫耐受；坐骨神经

Objective An exploration of using flow cytometryto measure DNA aneuploidy,cell cycle,and Bcl-2level in pleurorrhea exfoliated cell to help diagnose lung cancer.

目的 探讨用流式细胞术测定胸水脱落细胞DNA异倍体、细胞周期、Bcl-2水平，采用平行试验对肺癌诊断的价值。

The result using inhibitors of Ca〓 channels supported above observation. When Ca〓 channels in plasma membrane were blocked by LaCl〓, verapamil or nifedipine, and the Ca〓 channels in endomembranes opened naturally, extracellular CaM couldn't induce 〓 increase. When Ca〓 channels in endomembranes were blocked by ruthenium red and the Ca〓 channels in plasma membrane opened naturally, extracellular CaM induced Ca〓 increase in guard cells, but the extent of Ca〓 increase was lower than that of control.

使用质膜Ca〓通道抑制剂LaCl〓、异博定、尼群地平及细胞内Ca〓通道抑制剂钌红的结果支持这种推测：在质膜Ca〓通道被阻断、细胞内Ca〓通道仍正常开放的情况下，保卫细胞〓不能被CaM诱导增加；细胞内Ca〓通道被抑制、质膜Ca〓通道正常开放的条件下，细胞外CaM诱导保卫细胞内〓升高的幅度不能达到不使用Ca〓通道抑制剂的水平。

Sodium propionate and sodium pyruvate could promote liver gluconeogenesis by up-regulating the abundance of PEPCK-C mRNA in the hepatocytes, but the up-regulation was limited.

表明，丙酸钠、丙酮酸钠能通过上调体外培养的新生犊牛肝细胞PEPCK-C mRNA的表达而促进肝糖异生代谢，但上调作用是有限的；胰岛素能通过下调体外培养的新生犊牛肝细胞PEPCK-C mRNA的表达而抑制肝糖异生代谢，且下调作用呈剂量依赖性；胰高血糖素与胰岛素作用刚好相反；瘦蛋白未起直接的调节作用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。