异细胞的

The results of character evolution history reconstruction shows the SC in PT and AD clade might be a homoplasy character; also, the SC in PT clade might have multiple origins. SC in Pteridaceae has some special characteristics:(1) the cell long axes usually run parallel to the lateral veins;(2) Most of Pteris and Adianum species have VSC only in lower epidermis;(3) Species have ISC also have VSC.(4) Presence of spicular cells is highly correlated with the long veinal epidermal cells. Species have type III silica deposition type usually have longer and narrower veinal epidermal cell compared with ordinary epidermal cells; Species have type IV silica deposition type also have long epidermal cells, but not significantly different from ordinary epidermal cells.

本实验中亦发现凤尾蕨科之矽异形细胞具有几项特色：(1)矽异形细胞之长轴多与叶片侧脉方向平行(2)发现多数铁线蕨属以及凤尾蕨属植物仅下表皮具有脉上矽异形细胞；(3)具有脉间矽异形细胞的种类会同时具有脉上矽异形细胞；(4)长条形的侧脉上表皮细胞与矽异形细胞具有相关性，仅具脉上矽异形细胞的分类群之脉上表皮细胞均较一般表皮细胞细长；具有脉间矽异形细胞之物种之脉上表皮细胞亦为长条形但形态与一般表皮细胞常无明显形态差异。

Idioblast Any specialized plants cell that is dispersed among cells of a very different kind.

异细胞：分散在细胞群中呈现多种类型的所有特殊的植物细胞均称异细胞。

METHODS: A series of N-octyl-N'-succinyl chitosan labeled with fluorescein, were incubated with HepG2 liver cancer cells, K562 leukemia cells, A549 human lung cancer cells, and BGC cancer cells respectively and followed by using flow cytometry and enzyme-linked immunosorbent assay to assess the affinity and inhibition of N-octyl-N'-succinyl chitosan for the four kinds of tumor cells already mentioned.

用异硫氰酸荧光素对N-正辛基－N'-琥珀酰基壳聚糖进行标记，再分别与人肝癌细胞(HepG2)、人白血病细胞(K562)、人非小细胞肺癌细胞(A549)及人胃癌细胞共培养，通过流式细胞仪及酶联免疫检测仪测定N-正辛基－N'-琥珀酰基壳聚糖对肿瘤细胞的亲和性及抑制力。

In this study, Mono-nuclear cells containing hematopoietic stem/progenitor cells were isolated from umbilical cord blood of normal full-term deliveries, healthy adult and heterocellular HPFH patient bone marrow by density gradient centrifugation. After simple purification and expansion in vitro, MNCs were induced to erythroid cell differentiation using different culture systems and cytokines including Epo, IL-3 and GM-CSF.

本研究首先利用密度梯度离心方法，从正常成人和异细胞型遗传性胎儿血红蛋白持续存在综合症家系患者的骨髓组织、健康足月正常分娩胎儿脐带血中分离得到了含有造血干／祖细胞的单个核细胞(Mono-Nuclear Cell，MNC)，进行初步纯化及体外扩增后，组合使用Epo、IL-3、GM-CSF等细胞因子，将造血干／祖细胞向红系方向进行诱导培养。

RESULTS:Isoalantolactone exhibited excellent anti-proliferative activities on HeLa, SHIN3 and HOC-21 and HAC-2 cell lines, while 1-O-acetylbritannilactone and britannilactone demonstrated weak anti-proliferative activities on HeLa, SHIN3, HOAC-21 and HAC-2 cell lines even at the concentration of 100 μmol/L. The apoptotic rate in isoalantolactone group with the concentration of 12.5 μmol/L was higher than that in PBS group.

结果： 异土木香内酯对上述5种肿瘤细胞的增殖均有明显的抑制作用，1-氧-乙酰大花旋覆花内酯和大花旋覆花内酯即使在100 μmol/L的高浓度下，对5种妇科肿瘤细胞的增殖不显示抑制活性；流式细胞学检查发现异土木香内脂处理后的HeLa细胞出现凋亡现象。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

To determine the effects of ovarian steroid hormones and some cytokines on the expression of RANTES in cultured cells METHODS AND RESULTS The study included the following two parts 1 The Protein Production of RANTES in Peritoneal Fluids and the Expression of RANTES mRNA in Eutopic and Ectopic Endometrium of Patients with Endometriosis Twenty-eight patients with endometriosis were selected as study group and twenty patients without endometriosis and maglinant disease were selected as control group . In our study, 10 samples of peritoneal fluid in Em and 10 samples of in En were taken, 13 specimens of ovarian endometrioma , 3 red peritoneal endometriotic lesions , 2 uterosacral ligament nodules , 17 matched eutopic endometrium and 12 specimens of endometrium in En were biopsied.

研究目的 1 从蛋白水平检测RANTES在子宫内膜异位症患者腹水中的表达，从核酸水平比较RANTES在内异症患者的异位病灶和在位内膜与非内异症患者子宫内膜之间表达的差异，探讨RANTES在内异症患者盆腔炎性反应中的作用；2 体外培养在位和异位的子宫内膜细胞，研究细胞因子TNFα、INFγ、雌孕激素以及PPARγ激动剂15-d-PGJ〓对RANTES表达的影响，探讨这些因素在内异症发病机理及内异症患者盆腔炎性反应中的作用。

D. The function of stimulating xenogenous lymphocyte proliferation was the same between peripheral DCs and ascites PCs. E. The percentage of CD3〓CD56〓 cells was the same in CIK cells co-culture with DCs transfected with SKOV3 RNA, CIK cells co-culture with DCs, and CIK cells. F. The expansion rate of CIK cells can be accelerated by co-culturing with loaded or unloaded DCs. However, the expansion rate between loaded or unloaded group is the same. F. The strongest cytotoxicity against SKOV3 cell line was achieved by CIK cells co-cultured with DCs loaded with SKOV3 lysate.

结果：1、腹水可获得0.83±0.24×10〓个AMC/ml，单核细胞有0.74±0.25×10〓个/ml；2、卵巢癌患者外周血可获得0.87±0.20×10〓个AMC/ml，单核细胞有0.92±0.17×10〓个/ml；3、除CD86外周血单核细胞来源DC表达较高以外，其他表面分子在不同来源DC间没有统计学差异；4、不同来源DC的异基因刺激能力没有差异；5、与负载或未负载卵巢癌抗原的DC共培养并不能提高CIK细胞群中CD3〓CD56〓细胞的数量；6、CIK细胞增殖显著，培养14天时可扩增19.18±4.70倍，培养21天时可扩增35.82±4.36倍；7、与未负载或负载DC共培养的CIK细胞在培养第14天后增殖速率大于单纯CIK细胞。

Results: Compared with control group, apoptosis cells increased from 0.5%to 10%(some even to 15%) after 24,48 and 72 h action of -300,-500 and -1 000 V electrets. After action of -500 V PTFE electrets for 48-72 h, fibroblast cells showed characteristic morphological features of apoptosis. These features included chromatin aggregation, nuclear and cytoplasmic condensation and partition of cytoplasm and nucleus into membrane bound-vesicles.

结果：-300、-500和-1 000 V驻极体作用成纤维细胞24、48和72 h以后，与对照组相比，成纤维细胞的凋亡量从0.5％增至10％（部分可达15％）；驻极体作用成纤维细胞48～72 h，出现细胞凋亡特有的形态学特征，即：细胞异染色质边集，细胞裂解，可见凋亡小体。

Result Obvious immunological rejection occurred after implantation of aortic and pulmonary homografts. Cellular and humoral immunological responses were involved in the process of chronic immunological rejection.

结果 低温保存同种带瓣管道移植后有明显的免疫排异反应发生，细胞和体液免疫参与免疫排异反应的过程；慢性免疫排异反应以细胞免疫为主。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。