异细胞

It is reported that alkannin and shikonin isolated from the roots of Lithospermum erthyrorhizon or Alkanna tinctoria and their acylshikonin derivatives are potent selective inhibitors of DNA Topoisomerase Ⅰ.

文献报导紫草素和酰基紫草素等具有DNA拓扑异构酶Ⅰ的选择性抑制活性。本组曾合成得到少量环异紫草素（8），它对小鼠白血病P-388肿瘤细胞具有强烈的抑制作用。

The Flow Cytometry has been widely used to determine the heterotrophic bacteria in water environment presently. Also, it can be used to determine the heterotrophic bacteria in benthos environment after the appropriate pretreatment.

目前流式细胞术已被广泛应用于水体异养细菌的检测，采用适当的前处理方法可将黏附在沉积物颗粒上的异养细菌提取到水相中进而利用FCM进行检测。

MTT assay indicated that anti-proliferative activities in HeLa, SHIN3, HOAC-21 and HAC-2 cell lines were closely related to structure, 1,10-seco of eudesmane sesquiterpene lactone resulted in reduced anti-proliferative activity. The anti-tumor activity of isoalantolactone was probably achieved by inducing HeLa cell apoptosis.

提示A-环开环可能是导致桉叶烷型倍半萜内酯的体外抑制肿瘤细胞增殖作用消失或减弱的原因；异土木香内酯抑制肿瘤细胞增殖作用有可能是通过诱导肿瘤细胞凋亡机制而实现的。

The cell suppression ratio and surviving ratio were detected respectively under conditions of simple hypoxia, or 5mmol/L glucose complicated with hypoxia. The mRNA levels of key enzymes that are involved in regulating glyco-metabolism were detected, including the mRNA levels of pyruvate-kinase, hexokinase, succinic dehydrogenase and isocitric dehydrogenase, and the activity of lactate dehydrognase.

以SMMC-7721人肝癌细胞和L02正常肝细胞作为研究对象，分别在单纯缺氧及加葡萄糖缺氧条件下，观察细胞生长，并检测糖代谢关键酶：丙酮酸激酶(pyruvate-kinase， PK)、己糖激酶(hexokinase， HK)、琥珀酸脱氢酶(succinic dehydrogenase， SDH)、异柠檬酸脱氢酶(isocitric dehydrogenase， IDH) mRNA表达水平和乳酸脱氢酶(lactate dehydrogenase， LDH)活性。

The cytomorphological changes of K562 cells were observed in the electron microscope.Results RRTJ at the concentration of 1μL/mL expressed obviously inhibitory effect on the growth of K562 cells.The inhibitory effect at the concentration of 2μl/ml went up to its highest at 83.4%.RRTJ showed virulence to K562 cells.The karyon changed because of RRTJ attending.

结果 每mL培养液含1μL刺梨汁即对肿瘤细胞增殖有明显的抑制作用，每mL培养液含2μL刺梨汁抑制作用达到最大，为83.4%，再加大剂量抑制作用无明显升高，各种剂量的刺梨汁对细胞均无毒性，刺梨汁的加入使得细胞体积明显变小，细胞核形态发生改变，异染色质增多。

Objective An exploration of using flow cytometryto measure DNA aneuploidy,cell cycle,and Bcl-2level in pleurorrhea exfoliated cell to help diagnose lung cancer.

目的 探讨用流式细胞术测定胸水脱落细胞DNA异倍体、细胞周期、Bcl-2水平，采用平行试验对肺癌诊断的价值。

To explain relationship between the effect of CLD on senile Demention and neuron in hippocampal area. The change of natural aging mice's neuron in hippocampal area were studied by applying microscope and transilluminating electric microscope. Results: Comparing with the young control, the old control mice's hippocampus had less small pyramid cells, the nerve cells denaturalizing and their branches reducing in the microscope. Hippocampal nerve cells were degenemiceing, with nucleus membrance crinkling like wave shape were not clear, and chromatin condensing with high electron density, heterochromatin accruing, part cell organs inclosing nucleus, nucleus condensing in the EM.

为了阐明菖龙丹抗老年性痴呆作用与海马区神经元结构的关系，运用光镜和透射电镜观察了菖龙丹对自然衰老小鼠海马区形态的影响，结果显示老年对照组小鼠光镜下海马锥体细胞较青年对照组数量减少，细胞体积变小，神经元变性，分枝减少；电镜下海马神经细胞发生退化改变，核膜皱缩，呈波浪状，膜结构不清晰，核染色质浓缩，电子密度高，异染色质较多，部分胞浆细胞器向细胞核聚集，有的可见细胞核固缩。

In conclusion, species-specific responses of hepatocytes to cytokines and interactions with xenobiotic metabolites may limit the clinical effectieness of porcine hepatocytes in BAL.

结论，肝细胞之间对细胞因子的物种特异性反应及对异源性代谢产物的相互作用可能限制了猪肝细胞作为生物人工肝在临床的应用效果。版权声明：本译文文字内容归丁香园网站（http：//www.dxy.cn）所有，未经网站书面许可，请勿转载或摘编。

The drugs which had relationto mdrl and MRP shoud be put aside and other resistance mechnisms shouldbe considered.9.The middle doses of radiation had not remarkable effects to the resistancegene expression of mdrl and MRP in the living cells and recured cells.

中等剂量放疗后对活细胞的耐药基因mdrl和MRP的表达影响较小，放疗后复发细胞的药物敏感性无改变，复发细胞加速生长，可能对异搏定等耐药逆转剂效果减低。lO。

In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.

以牛泡沫病毒(Bovine foamy virus， BFV)中国株BFV3026原病毒DNA为材料，构建R区系列缺失质粒，通过对其转染细胞中RT水平及对缺失质粒与luc报告质粒共转染细胞中萤火虫荧光素酶活性的测定，确立U5区对于BFV3026两类启动子LTR和IP均具有负调控作用；同时将带有不同R区的BFV3026结构基因片段克隆于异源启动子CMV之下，通过对其转染细胞293T中RT酶活性的测定，确立R区对于病毒结构基因pol的表达具有一定的调节作用，并将其功能区域初步界定在R区5′端100bp内。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新