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异细胞

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The results indicated that the expression level of cgi-100 decreased in K562 cells treated with matrine. Heterochromatin decreased, euchromatin and the proportion of S phase in K562-cgi-100 cells increased, and cell proliferation enhanced.

结果表明,在苦参碱作用K562细胞后cgi-100基因表达下调;重组K562细胞中cgi-100基因过表达,且常染色质增加,异染色质减少,细胞增殖旺盛,S期细胞增多,增殖速度高于对照组。

At 12w after operation, the cells in control group still behaved as fibroblasts and fibrocartilage like cells were predominant in grafts of cell and gene groups , the grafts in these two groups were similar to normal ligament morphologically.

手术后12周,对照组显现移植物内有大量的成纤维细胞,甲苯胺兰染色轻度异染;而细胞组和基因组细胞量减少,显现类软骨性状,细胞排列与正常前交叉韧带接近。

The re was no significant difference between the contents of SP and VIP of lung tissue in experiment group and those in control group.4. Ultrastructurally, after infected with IBV, lots of grume were observed on the surface of trachea tissue and the cilia of the trachea were massed.

免疫电镜观察结果显示,大多数肾小管上皮细胞胞浆内均见有SP及VIP阳性颗粒,在上皮细胞间还可见有DNES细胞;肺脏中异嗜性白细胞颗粒也常呈SP及VIP阳性反应,肺脏内也可见DNES细胞。

Classification examination was put into practice for only normal cells observed.For the dubious specimen,tuberculosis-Ab,acid-fast bacteria staining,flow cytometry,CD series,gene,DNA aneuploidy,ZPP and tumor markers etc were detected. Results Tuberculosis lymph gland inflammation coverd highest rate:546 cases (36%) in tuberculosis node was 252 cases (46.2%),lymphonode period was 184 cases (33.7%),early tuberculosis lymphagland inflammation was 233 cases (44.7%).

利用改良一次性5~10ml注射器(22G、7号注射器,外径<0.7mm),针吸取材、涂片、行改良瑞-姬双染技术,低倍镜查找特殊异常细胞,油镜仔细观察细胞形态结构特征,对于未见异常细胞要进行分类检查,对疑难标本,同时做结核抗体、抗酸菌染色、流式细胞仪、CD系列、基因、DNA异倍体、锌原卟啉、肿瘤标志物等检测指标。

Part one Isolating, culturing, chondroblast induction and evaluation of human MSC in vitroObjective:To examine the property and proliferation ability of primary culturing MSC ,mvestgate the effect of lightly centrifugation and chemical definition medium to induce MSC differentiate into chondroblast.

结果:原代骨髓基质干细胞易于体外培养,增殖旺盛;经轻度离心和化学限定培养基诱导后细胞呈高密度生长,仍保持较旺盛的增殖能力,细胞转化成圆形肥大细胞,甲苯胺蓝染色显示软骨细胞外基质 v的特异性异染,11胶原蛋白免疫细胞化学染色呈强阳性,BT干CR鉴定显示*胶原mRNA丰富的表达。

In the BC〓F〓 and BC〓F〓 generation of Yannong15×Thinopyron intermedium hybrid, 4 octoploids which had chromosome number of 2n=56 and could form 28 bivalent at PMC MI, 14 disomic addition lines which had chromosome number of 2n=44 and could form 22 bivalent at PMC MI; three monosomic addition lines which had chromosome number 2n=43 and had the chromosome configuration of 21Ⅱ+1Ⅰ at PMC MI, 1 substitution line which had chromosome number 2n=42 and could form 21 bivalent at PMC MI were selected by observation of mitosis in root tip cell and meiosis in pollen mother cell.

在烟农15与中间偃麦草杂交的BC〓F〓和BC〓F〓代中,通过根尖有丝分裂和花粉母细胞减数分裂中期Ⅰ观察选出4个2n=56,PMC MI染色体构型为28Ⅱ的八倍体小偃麦;14个2n=44,PMC MI染色体构型为22Ⅱ的双体异附加系;3个2n=43,PMC MI染色体构型为21Ⅱ+1Ⅰ的单体异附加系;1个2n=42,PMC MI染色体构型为21Ⅱ的异代换系。

Methods To collect 10 specimen residual ear cartilage of microtia patients about 8-year-old and cultured in vitro, dye with toluidine blue and immunohistochemistry of collagen type II.

结果:残耳软骨细胞在体外培养时随代数增加呈异染陛的糖胺多糖减少,第1、3代Ⅱ型胶原阳性细胞比例约68%~71%,第5代为23%、显著降低;冻存后第3代为65%,与同代数的细胞没有差别,100ng/mi浓度的CDMP-1可以将第4代细胞的阳性比例维持在66%,而且冻存和生长因子不会引起细胞周期的变化。

Nither CDMP-1, nor cryopreservation can effect cell cycle of the chondrocytes.

结果:残耳软骨细胞在体外培养时随代数增加呈异染性的糖胺多糖减少,第1、3代Ⅱ型胶原阳性细胞比例约68%/~71%,第5代为23%、显著降低;冻存后第3代为65%,与同代数的细胞没有差别,100ng/ml浓度的CDMP-1可以将第4代细胞的阳性比例维持在66%,而且冻存和生长因子不会引起细胞周期的变化。

The follicle cells of growing follicle were composed of small cells, intermediate cells and pyriform cells.

乌梢蛇生长卵泡的滤泡细胞是由小细胞、中间细胞和梨形细胞组成的异形滤泡细胞。

As exposed to optics microscope and inverted microscope,compared to control,SW480 cells with Giemsa staining that treated with the H2RA(cimetidine、nizatidine) took on malignance declining as crimpled firstly,cellular bulk and heteromorphism dimimished,the amount of rounded cell manifolded,nucleolus bulk lessened,dyeing thin,the amount of nucleolus decreased,partial cell organs manifest side-gathering phenomenon on chromatin,and the link between SW480 cell is loosened , subsequently partial rounded cell is floated.When the H2RA(cimetidine 40μM、nizatidine 20μM) against SW480 cells after 3 day,SW480 cells change roundly,nucleolus smashed,shrinking nucleolus came into being,and part of those not adhibited cell wall.

在光镜和倒置相差显微镜下,与对照组相比,经姬姆萨染色观察,西咪替丁、尼扎替丁处理的SW480细胞,先发生皱缩,体积变小,异形性减少,细胞变圆数目增多,细胞核变小,染色变淡,核仁数量减少,部分可见染色质边集现象;细胞间连接疏松,随后部分变圆细胞浮起。40μM西咪替丁及20μM尼扎替丁连续处理3天后,SW480细胞全部变圆,细胞核碎裂,固缩微核形成,部分不再贴壁。

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