异细胞

In part of edible fungi, two different nuclei in heterokaryon are generally consided to distribute evenly to all of colony through clamp connection, and the ratio of two nuclei should be 1:1. But other hypothesis was demurrable according aberrancy of nucleus ratio during monokaryotization of oidiospore and protoplast in F. velutipes. My experiment was constituted of two parts.

在部分食用菌中，一般认为异核体菌丝通过锁状联合这一细胞结构将两种核均匀分布到全部菌落，异核体中两种核的数量比例应该是1：1的关系；但金针菇原生质体单核化和粉孢子单核化过程中发生的核偏离的研究表明，异核体菌丝两种核的数量比例可能不是1：1的关系。

Those suggest that the two capsules both can control the growth of ectopica endometrium by regularing patients endocrine.At the same time,they both increase the activity of NK cells to reinforce the effect of getting rid of the ectopia endometrium.

这说明桂附胶囊和理气胶囊均能通过调节内分泌而抑制异位内膜的血管形成，从而抑制异位内膜的生长；同时增加NK细胞的活性，以加强对异位内膜组织的清除作用。

FCM outcome show that osteosarcoma cell DNA become heteroploid and DNA content is abnormal. Compare with normal muscle cell, the G1 phase cell number of osteosarcoma decrease, however the S phase cell number increase significantly.

流式细胞术检测结果显示：骨肉瘤细胞DNA均呈异倍体改变，DNA含量异常，与正常肌肉细胞相比，骨肉瘤G1期细胞数减少，而S期细胞数显著增多。

Cobra venom factor was given to prevent hyperacute rejection and FK506 was given to prevent cellular rejection. The xenograft recipients were killed and cardiac xenografts harvested 4, 8, 12, 16, 24 hours after transplantation. Histologic examination was used to demostrate the degree of rejection and coagulation while revers transcriptase-polymerase chain reaction was used to detect the expression of tissue factor mRNA, and the guinea pigs hearts, harvested but not transplanted, were studied as controls in all samples.

建立豚鼠－大鼠异种异位心脏移植动物模型，使用CVF抑制超急性排异反应，FK506抑制细胞免疫排斥反应，分别在移植术后4、8、12、16、24h切取移植心脏，通过病理切片观察排异反应和凝血强度，同时通过RT-PCR方法检测组织因子mRNA表达强度，未移植豚鼠心脏作对照。

Studies also indicated that genistein exsists not only in soybeans but also in many kinds of plants such as vegetables and fruits.As a potential anticancer agent, the actional mechanisms of genistein mainly includes as follows:First, genistein can depress the activity of protein tyrosine kinase and transduction pathways for the phosphorylation of receptors and mitosis signal. So genistein can lead to cells' proliferation depressed. Second, genistein has minimal effects of phytoestrogens. It can be combined with estrogen receptor and improve the synthesis of cellular sex hormone binding glulobin, and improve the activity of UDP-glucuronyl transferase. Through these pathways, it can inhibit the cell activity of breast cancer and prostate cancer.

作为一个很有潜力的抗肿瘤物质，三羟异黄酮的作用机制主要包括：①抑制蛋白酪氨酸激酶活性，可阻抑PTK引起的受体磷酸化和有丝分裂的信号传递，导致癌细胞增殖受抑；②弱雌激素作用：可通过与体内雌激素受体结合，并可增加细胞内性激素结合球蛋白的合成，增加UDP-葡糖醛酸转移酶的活性等途径抑制乳腺癌和前列腺癌细胞活性；③拓扑酶Ⅰ和Ⅱ抑制剂，抑制细胞活性；④上调细胞周期性负性调节因子P21WAF1/CIP1的表达，使之负性调节因子作用增强；⑤可阻止胰岛素样生长因子-Ⅰ、肝细胞生长因子和神经生长因子的作用而抑制肿瘤生长；⑥其他：抗氧化作用、抑制热休克蛋白、诱导细胞凋亡、抑制新生血管生成和抑制多种耐药相关蛋白等。

METHODS: Benzhydroxamic acid derivative E was artificially synthesized. The gastric cancer line 7901 was selected to serve as the target cell. The effect of medicine E on tumor cell activity was examined by cell culture technology in vitro. The change of morphology and the ultrastructure after treatment with medicine E was observed with the optic microscope and the electron microscope.

以胃癌细胞株7901为靶细胞，采用体外细胞培养技术评价苯异羟肟酸衍生物E对肿瘤细胞活性的影响，通过光学和电子显微镜对E药物作用后细胞形态学进行观察，用免疫组化和RT PCR方法检测细胞凋亡。

The leaf epidermis structures of 6 species of Rhododendron had been observed under LM and SEM. The results show that all share together common features as follows: the leaf blades are typical back-abdomen bifacial types, the upper epidermis are formed by two or three layer bigger cells which inside layer cell is bigger than outside one, and the upper epidermis are covered with thicker cuticle.

利用光学显微镜和扫描电子显微镜观察了6种杜鹃属植物的叶片结构，结果表明，它们具有共同形态结构特征：典型的异面叶，上表皮由2~3层细胞组成，内大外小或内、外等大，有较厚的角质膜，无气孔器分布；下表皮均由1层细胞组成，排列紧密；栅栏组织由2层以上长柱形细胞组成，排列紧密；海绵组织细胞较短，排列较疏松，细胞间隙较大；均有表皮附属物。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group）.The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group）.The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组（K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组），采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量，流式细胞术检测细胞内阿霉素的浓度，基因、酶学、蛋白水平检测实验分3组（K562组、K562/A02组和K562/A02+TTD组），采用RT-PCR法检测mdr1 mRNA的表达，免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平，Western-blotting法检测P-糖蛋白和bcl-2表达。

Results: Compared with control group, apoptosis cells increased from 0.5%to 10%(some even to 15%) after 24,48 and 72 h action of -300,-500 and -1 000 V electrets. After action of -500 V PTFE electrets for 48-72 h, fibroblast cells showed characteristic morphological features of apoptosis. These features included chromatin aggregation, nuclear and cytoplasmic condensation and partition of cytoplasm and nucleus into membrane bound-vesicles.

结果：-300、-500和-1 000 V驻极体作用成纤维细胞24、48和72 h以后，与对照组相比，成纤维细胞的凋亡量从0.5％增至10％（部分可达15％）；驻极体作用成纤维细胞48～72 h，出现细胞凋亡特有的形态学特征，即：细胞异染色质边集，细胞裂解，可见凋亡小体。

The result using inhibitors of Ca〓 channels supported above observation. When Ca〓 channels in plasma membrane were blocked by LaCl〓, verapamil or nifedipine, and the Ca〓 channels in endomembranes opened naturally, extracellular CaM couldn't induce 〓 increase. When Ca〓 channels in endomembranes were blocked by ruthenium red and the Ca〓 channels in plasma membrane opened naturally, extracellular CaM induced Ca〓 increase in guard cells, but the extent of Ca〓 increase was lower than that of control.

使用质膜Ca〓通道抑制剂LaCl〓、异博定、尼群地平及细胞内Ca〓通道抑制剂钌红的结果支持这种推测：在质膜Ca〓通道被阻断、细胞内Ca〓通道仍正常开放的情况下，保卫细胞〓不能被CaM诱导增加；细胞内Ca〓通道被抑制、质膜Ca〓通道正常开放的条件下，细胞外CaM诱导保卫细胞内〓升高的幅度不能达到不使用Ca〓通道抑制剂的水平。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新