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By phenotype observation, combined with root tipchromosome counting, PMC MI pairing analysis and chromosome C-banding, a ditelosomic addition line 94K227 and a disomic addition line 94K280 was identified among BC2F2 and BC2F3 population respectively.C-banding results showed that 94K227 contains 1 pair of long arms in chromosome B, and 94K280 contains 1 pair of chromosome D from R.

用普通小麦-纤毛鹅观草双倍体与普通小麦中国春连续回交两次,然后连续自交,通过形态观察、根尖细胞染色体计数、花粉母细胞减数分裂中期Ⅰ染色体配对分析及染色体C-分带,在BC2F3和BC2F3群体中,分别筛选到一个端二体异附加系94K227和一个二体异附加系94K280,其中C-分带显示94K227添加的是纤毛鹅观草染色体B的一对长臂, 94K280添加的是纤毛鹅观草的一对染色体D。

After discussion of the relationship among idioblastic cells,tolerance to abiotic stress and betaine,the author thought the reason of transgenic plants having an increased number of idioblastic cells was that BADH was introduced and much betaine might be synthesized.

通过对异形细胞、植物抗逆性、甜菜碱三者之间的关系讨论,认为转基因植株异形细胞数量多是由于BADH基因的导入可能合成了较多甜菜碱。

Methods:The subcutaneous adipose tissue was obtained from the fold inguen of four SD rats, then digested with collagenase type Ⅰ and cultured in endothelial medium for seven days.

获取4只雄性SD大鼠鼠蹊部皮下脂肪,通过机械分割和组织消化法获取脂肪基质细胞,在内皮细胞培养液中培养7d后,经免疫细胞化学及透射电镜鉴定证实95%以上细胞已具内皮细胞表型特征,再将这些细胞进行成脂(DMEM/F-12培养基内加入0.5mmol/L1-甲基-3-异丁基-黄嘌呤,1μmol/L地塞米松,10μmol/L胰岛素,200μmol/L吲哚美星)定向诱导7d,形态学观察和油红O特殊染色鉴定诱导后的细胞。

Results:(1) CBC possesses a natural trabecular structure. Its components is similar to the mineral of bone.(2) CBC has great cellur biocompatibility. It can promote cell adhesion, proliferation and secretory while needn t modification.(3) In nude mice, the ectopic osteogenesis of MCCAB was obvious which was better than that of CBC alone and the new bone formation increased in pace with time. In the MCCAB, both intramembranous and cartilaginous osteogeneses were seen but the former dominated.(4) When MCCAB was autotransplanted intramuscularly, the osteogenesis was much better than that of negative control groups.(5) The MCCAB can repair autogenous calvaria defects effectively and the effect was much better than that of CBM alone and blank group. Conclusion:(1) CBC possesses good biocompatibility, osteoconduction and porous structure, can be used as scaffold material for constructing of tissue engineering bone.

结果:(1)CBC具有天然的多孔网状结构,最大程度地保持了骨矿的微结构特征,可以作为骨组织工程的支架材料;(2)CBC细胞亲和性好,不需作修饰即能促进成骨细胞的粘附、增殖和分泌活动;(3)MSO/CBC复合人工骨在裸鼠皮下异位成骨作用明显,兼有膜内成骨和软骨内成骨的特点,成骨效果明显优于单纯CBC植入组;(4)MSO/CBC复合人工骨兔自体肌袋异位成骨作用明显,血管化充分,成骨效果明显优于单纯CBC植入组;(5)MSO/CBC复合人工骨修复兔颅骨临界性缺损效果好,明显优于单纯植入CBC组和空白对第四旱区大学硕士学位论文一照组,可以作为骨移植替代材料用于骨缺损畸形修复。

Moreover, the preliminary test of the biological activity of the 8 target derivatives showed that 7-(2-adenine)ethoxyl-2-ethyl-4"- methoxy isoflavone and 7-(2-hydroxyl-3-piperidino)propoxy-2-ethyl-4"- methoxyl isoflavone have fine biological activities on suppression of the growth of MCF-7 cell in breast cancer and the hela cell in cervical cancer of human being.

另外还对8个目标衍生物进行了初步的药理活性试验,发现7-(2-腺嘌呤)乙氧基-2-乙基-4′-甲氧基异黄酮和7-(2-羟基-3-哌啶)丙氧基-2-乙基-4′-甲氧基异黄酮均有较好的抑制人乳腺癌MCF-7细胞和人宫颈癌hela细胞生长的药理活性。

Besides, benzyl isothiocyanate and phenethyl isothiocyanate also decreased the levels of mitochondria membrane potential, induced reactive oxygen species and calcium ion production in examined DU-145 cells.

由结果显示,苯甲基异硫氰酸酯和苯乙基异硫氰酸酯确实会造成人类前列腺癌细胞株DU-145细胞周期停滞以及诱导细胞凋亡的产生。

With CYP1A2, 3-fold enhancement in actiity per cell was seen for conerting ethoxyresorufin to resorufin compared to C3A cell monolayers.

结果发现,每个细胞中CYP1A2将乙氧基-异吩唑酮转化为9-羟基-3-异吩恶唑酮的能力相对于单细胞层的C3A细胞增强了3倍。

Results In 3T3-L1 adipocytes, isoproterenol at the concentration of 10 μmol/L reduced the adiponectin secretion and insulin at the concentration of 5 nmol/L could block the inhibitory effect of isoproterenol. In 313-Li adipocytes expressed myrAkt, adiponectin secretion decreased significantly, isoproerenol treatment increased adiponectin and insulin couldn't block the effect of isoproterenol.

结果 异丙肾上腺素可明显降低3T3-L1脂肪细胞脂联素的蛋白质表达,胰岛素可纠正这一变化;表达myrAkt的3T3-L1脂肪细胞中脂联素明显减少,异丙肾上腺素可增加脂联素蛋白的表达,胰岛素不能纠正这一变化。

The cells may be autologous, syngeneic, allogeneic or xenogeneic, as tolerance to foreign cells is created in the patient during reactivation of the thymus.

因为患者在胸腺重新活化过程中产生外来细胞抗性,因而这些细胞可以是自体的、同种同基因的、同种异基因的、或异种异基因的。

Sodium propionate and sodium pyruvate could promote liver gluconeogenesis by up-regulating the abundance of PEPCK-C mRNA in the hepatocytes, but the up-regulation was limited.

表明,丙酸钠、丙酮酸钠能通过上调体外培养的新生犊牛肝细胞PEPCK-C mRNA的表达而促进肝糖异生代谢,但上调作用是有限的;胰岛素能通过下调体外培养的新生犊牛肝细胞PEPCK-C mRNA的表达而抑制肝糖异生代谢,且下调作用呈剂量依赖性;胰高血糖素与胰岛素作用刚好相反;瘦蛋白未起直接的调节作用。

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