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The isothermal amplification step depends not only on the replication of DNA polymerase, but also on the scission of nicking enzyme, which can generate a great number of aptamer.

我们用该方法对细胞色素P450酶系中的异喹胍羟化酶的单碱基多态性(CYP2D6*10)进行了检测,使突变型和野生型基因DNA得到了很好的区分。2。

Objective To investigate the value of sequential and quantitative analysis of donor chimerism to predict the outcome of allogeneic hematopoietic stem cell transplantation, to determine the optimal time point of adoptive immunotherapy and to estimate the efficacy of adoptive immunotherapy.

目的探究供体细胞嵌合率的动态定量检测在预测异基因造血干细胞移植后的转归,确定过继免疫治疗时机和评价AIT疗效中的作用。

The total RNA were extracted from CDV infected DK cells or the tissues of infected animals by single step method of acid guanidinium thiocyanate-phenol-chloroform extraction and was used for reverse transcription.

用异硫氰酸胍-酚-仿一步抽提法提取细胞总RNA进行反转录,再以此产物为模板进行PCR扩增,筛选出最佳扩增条件。

Moreover, RIM-BP3 and Hook1 mutant mice share several common abnormalities, in particular with regard to the ectopic positioning of the manchette within the spermatid, a presumable cause for the sperm head deformities.

而且,RIM-BP3和Hook1的突变小鼠具有一些共同的表型,尤其是精子细胞中精子领的异位分布,这是造成精子头部异常的潜在原因之一。

Spirogyra and DESMIDS.

异形配子形成后,通过形成细胞间接合管,接合成为受精卵。

Methods: Standard whole cell patch clamp technique was used.

采用全细胞膜片钳技术,观察不同浓度的异甘草素对ICa-L的作用。

Cholesterol contributes to the generation and development of atherosclerosis while non sterol isoprenoids play a role in regulation of cell proliferation, signal transduction and ge...

胆固醇在动脉粥样硬化的发生、发展中起重要作用,而异戊二烯则参与细胞增殖调节、信号转导及肿瘤发生过程。目前,该酶已成为一些有效的抗动脉粥样硬化药物治疗的靶点。

The inhibition of the cells during 60 hours was determined by melhylthiazolyl tetrazolium assay, and the cell growth curve was made.

目的:研究米非司酮和孕三烯酮对体外培养的异位的和正常的子宫内膜间质细胞生长增殖的影响。

Shift production site of PGs in ovary from granulosa cells to theca cells may be related to the pathogenesis of PCOS.

人卵巢组织的前列腺素可能在COX-1催化下于颗粒细胞生成;COX-1在卵巢组织上的异位表达,可能与PCOS发病有关。

However, the titration and protein concentration of IBDV in vero cells was higher than that in CEF cells.

因此选用异源的咖细胞大量增殖IBDV,并采用不同的方法进行纯化比较。

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