异细胞

The structure of cell wall was not destroyed by the algaecide. So we could know: after being contacted with the algae, the algaecide could penetrate the cell wall and reacted with the protein directly, damaging the function of cell and killing the cell at last.Furthermore, we blended the isothiazolone with the high polymer, such as chitin, chitosan and poly, and prepared the slow-release drug films.

实验发现，异噻唑啉酮对棕囊藻的细胞形态结构破坏程度不大，表明了异噻唑啉酮对藻体的细胞壁、细胞膜具有很强的穿透作用，能够快速穿透细胞壁和细胞膜，与细胞内部的蛋白质作用，抑制细胞生理功能的正常发挥，导致藻体死亡。

The cytotoxicity of these compounds were valuated by MTT and SRB methods using three cancer cell lines: HL-60, A-549, P-388. Cyclo-shikonin, and cyclo-alkannin have high cytotoxicity against HL-60.The chiral center of side chain has no effect on their activity. 2- 1-(Acetyloxy-4-methyl-3-pentenyl -5, 8-dihydroxy-1, 4-naphthoquinone (105) has strong inhibition effect on P-388 cells. 2- 1-(isooctyloxy-4-methyl-3-pentenyl -5, 8-dihydroxy-1, 4-naphthoquinone (108) and 2- 1-(2-furoyloxy-4-methyl-3-pentenyl -5, 8-dihydroxy-1, 4-naphthoquinone (112) have high inhibition on A-549.Shikonin (1), alkannin (2), and their derivatives have the structural features of a planar chromophores and short side chain. We examined the ability to inhibit the telomerase.

抗肿瘤活性初步筛选测试采用小鼠白血病P-388肿瘤细胞和人肺癌A-549肿瘤细胞进行，结果表明侧链羟基的手性对抗肿瘤活性无明显影响；有数个化合物具有很好的体外抑制活性，特别是化合物2-（1-异辛酰氧基-4-甲基-3-戊烯基）-5，8-二羟基-1，4-萘醌（108）和2-[1-（2-呋喃甲酰氧基）-4-甲基-3-戊烯基]-5，8-二羟基-1，4-萘醌（112）体外显示对人肺癌A-549肿瘤细胞有强效；2-（1-乙酰氧基-4-甲基-3-戊烯基）-5，8-二羟基-1，4-萘醌（105）对小鼠白血病P-388肿瘤细胞有强效；环紫草素和环异紫草素对人白血病HL-60细胞有强烈抑制作用。

Objective: The purpose of our studies are gaining a better insight into the effect of genistein on mesangial cell"s phenotype change and ECM expression plus degradation under DN circumstances.We add genistein with different concentrations in high glucose cultured rat mesangial cells;feed DM rats with the single concentration genistein for different days;to explore the expression of SMemb,the mark of mesangial cell"s hypertrophy phenotype change;to examine the synthesis of Fn as well as the change of the balance between MMP-2 and TIMP-l;to comprehend its effect on renal pathological changes of DM rats and some related factors;trying toexplore the possible mechanisms.Through these studies,we will have some information about the experimental value of genistein in DN field.

目的：为了研究金雀异黄素对DN环境下大鼠MC肥大表型转换标志物SMemb和ECM分泌及降解的影响，我们拟在细胞水平以不同浓度的金雀异黄素作用于高糖培养下大鼠MC，在动物水平以同一浓度的金雀异黄素作用于DM大鼠不同时间，观察细胞表型转换标志物SMemb、ECM主要成分Fn、ECM降解酶系统基质金属蛋白酶—2(MMP-2)／基质金属蛋白酶抑制剂—1(TIMP-1)表达的变化，同时观察金雀异黄素对DM大鼠肾脏病理及影响肾脏病进展的糖脂代谢紊乱的影响，并进一步探讨金雀异黄素发挥作用的可能机制；为明确金雀异黄素在DN治疗中的地位及意义提供实验依据。

In general, every 9-11 vegetative cells there was one heterocyst, But to wild-type filaments, every 12 vegetative cells. Mp22 has strong phenotype. Cells grow slowly under BG11 medium, BG11〓 medium, iron-deficiency condition and NaCl stress conditions in contrast with wild-type.

在BG11〓培养条件下，突变株也能诱导异形胞，但异形胞发育却有差异，端生异形胞很多，一般每隔9-11个营养细胞就有一个异形胞，但野生株一般每隔12个以上细胞才有一个异形胞。

Results The p53 gene mutation rate of colorectal cancer was 50%. The rate of apoptosis of tumor cells having mutation of p53 gene (22.11%) was much lower than that having no p53 mutation (40.57%, P＜0.05). Heteroploid cells had been found in 10/13 tumor samples with p53 gene mutation and 6/13 samples without p53 mutation (P＜0.05). The rate of apoptosis had no difference between the heteroploid cases and diploid cases.

结果 大肠癌标本p53基因突变率为50%；其中突变组肿瘤细胞凋亡率(22.11%)明显低于未突变组细胞凋亡率(40.57%)，(P＜0.05)；突变组异倍体细胞百分率(占76.9%)明显高于未突变组(占46.2%)，(P＜0.05)；异倍体肿瘤细胞凋亡率为34.0%，二倍体肿瘤细胞凋亡率为40.7%，二者差异无显著性(P＞0.05)。

The wood structures of Lauraceae were characterized by most diffuse porous; most pores solitary, less multiple and cluster pores; simple and scalariform perforation with few bars; most solitary pore; alternate intervessel pitting; most of rays multiseriate, less uniseriate; all of rays heterogeneous, mostly heterogeneous Ⅲ and Ⅱ type rays; most of axial parenchyma paratracheal type, less banded or marginal type; oil cell and mucilage cell in axial parenchyma cell or ray cell; mostly fiber tracheid and libriform fiber, less septate fiber.

结果表明：除檫木外，其它木材的管孔分布均为散孔材，具较高的单孔率；导管分子穿孔板兼有单穿孔和梯状穿孔2种或者仅具有单穿孔；导管间纹孔式为互列；导管－射线间纹孔式类型丰富，主要为刻痕状和大圆状。木射线有单列和多列射线2种类型，单列射线稀少、短，多列射线数量多；射线组织主要为异形Ⅲ和异形Ⅱ；轴向薄壁组织以傍管状为主，少数有带状或轮界状。油细胞和粘液细胞普遍存在于射线薄壁细胞或轴向薄壁细胞中。木纤维由韧性纤维和纤维管胞组成，部分树种具分隔纤维。

Each medicine used in the study totally has obivious effect on lens sodium-pump. Either on the molecular and cellular level or on the histological and organic level , they all have their distinctive demonstration. 1.Ouabain、Digoxine、DMSO decrease the expression of αsubunit of sodium pump on mRNA level , the cell morphology and ultramicroscopic structure demonstrated destructive effect,it indicates the cause of cataract of the medicines.2.D-thyroxine、Amphotericin B、Vitamin E increase the expression of αsubunit of sodium pump on mRNA level ,from the demonstration of the cell morphology and ultramicroscopic structure ,it indicates the medicines have protective or anti-distructive effect on lens.3.The medicines have isoform-specific action on lens sodium-pump,it indicates exploitting isoform-specific medicine has a promising clinical application.4. Lens sodium-pumps are inhibited probably lead to cataract, so in clinical medication, especially to the cardiovascular doctor, while he is using the sodium-pump inhibitors, he should pay more attention to adjust the dosage and duration of the drug therapy. 5. To creat the animal model of cataract through inhibiting lens sodium-pump, whether it can be a kind of classical method or not, it is worthy of further research.

本研究所选用的药物对晶状体钠泵均有明显的影响作用，在分子水平、细胞水平、组织器官水平均有特异的表现：1、哇巴因、地高辛、二甲基亚砜三种药物可降低晶状体钠泵α亚单位在 mRNA水平的表达，在细胞形态和超微结构上表现为损伤效应，提示了其潜在的导致白内障的可能。2、D甲状腺素、两性霉素B、维生素 E三种药物可升高晶状体钠泵α亚单位在 mRNA水平的表达，从细胞形态和超微结构上的表现，提示了其对晶状体有保护作用或抗损伤效应。3、药物作用于晶状体钠泵具有重整异构特异性，提示了开发出特异的具有重整异构作用的药物是极有临床应用前景的。4晶状体钠泵抑制后可能导致白内障的发生，故临床用药，尤其是心内科医生在使用这些钠泵抑制剂时应注意调整用药剂量及药物疗程。5 通过抑制晶状体钠泵来制作白内障动物模型能否成为一种经典的制作方法，还有待于进一步研究。

We named the spicular cells which forming false veins interveinal spicular cells, and the spicular cells on veins are veinal spicular cells in this thesis.

本论文将此构成假脉之细胞称为脉间矽异形细胞(interveinal spicular cell， ISC)，而将分布於叶脉上的矽异形细胞称为脉上矽异形细胞(veinal spicular cell， VSC)。

ResultsThe glandular cell in the endometrial implant after therapy with middle or high-dose of (10,15 gkg-1d-1) XiaoChaiHu Decoction showed characteristic features of apoptosis which displayed by the cell decreased in size, karyopyknosis , cytoplasmic and nuclear chromatin condensation , density increased and a lot of apoptotic bodies among cell. Whereas some stromal cell displayed degeneration and necrosis. The protein expression of Fas and Caspase-3 in endometriotic tissue of XiaoChaiHu Decoction group was higher than that in the endometrium.

结果中、高剂量小柴胡汤（10，15 gkg-1d-1）治疗后，异位内膜有较多腺上皮细胞出现凋亡的特征，表现为细胞体积变小，核固缩，胞浆和核染色质凝集，密度增高，细胞间凋亡小体，同时间质细胞中可见一些坏死细胞；异位内膜Fas蛋白、Caspase-3蛋白的表达水平明显高于其在位内膜。

Result Obvious immunological rejection occurred after implantation of aortic and pulmonary homografts. Cellular and humoral immunological responses were involved in the process of chronic immunological rejection.

结果 低温保存同种带瓣管道移植后有明显的免疫排异反应发生，细胞和体液免疫参与免疫排异反应的过程；慢性免疫排异反应以细胞免疫为主。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新