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In the studies on transferring and identifying alien chro-mosomes into wheat,the combined use of mitotic and meioticchromosome N-banding is also successfully practiced in deter-mination of T.durum-H.villosa amphipoid,five wheatalien disomic additions with H.villosa chromosomes,〓〓,alien sulstitution line with chromosome 〓 andsome translocation lines.

在外源染色体导入、鉴定研究中,利用根尖有丝分裂中期染色体和花粉母细胞减数分裂中期Ⅰ染色体的N-分带,快速、准确地鉴定了硬粒小麦一簇毛麦双二倍体、普通小麦一簇毛麦异耐加系V〓、V〓、V〓、V〓和V〓,普通小麦一簇毛麦异代换系V〓以及一些具有易位的杂种后代。

The findings were compared with those from ovarian carcinomas unrelated to endometriosis, atypical endometriosis, and ovarian endome...

结果:起源于内异症的卵巢子宫内膜样癌和卵巢透明细胞癌中各有1例检测到K-ras基因第12密码子发生突变,突变类型为GGT→TGT,即甘氨酸→半胱氨酸,其余标本均未见第12、13密码子突变。p21蛋白在与内异症相关的卵巢肿瘤。。。

Condition optimization for its efficient fermentation was also carried out. The reaction and separation system with the addition of resin HD-8 and the isoeugenol/aquoeus biphasic system of the biotransformation of isoeugenol to vanillin by Bacillus fusiformis CGMCC1347 were set up. The isolation and purification of isoeugenol and vanillin in both systems were studied. The reuse of the immobilized cells in biphasic system was successfully applied.

SW-B9的基础上,对该菌株进行了生理生化特性鉴定以及16S rDNA序列分析,命名为纺锤芽孢杆菌CGMCC1347;继而对该菌株的发酵条件进行了研究,首次建立了纺锤芽孢杆菌CGMCC1347转化异丁香酚生成香草醛的树脂分离耦合体系和异丁香酚—水两相体系,同时对此二体系的底物和产物的分离提取进行了研究,成功实现了两相体系中固定化细胞的反复利用。

In present study, we demonstrated that μ opioid receptor and D1 dopamine receptor could form heteromeric complexes in membranes preparation from HEK293T cells transiently cotransfected with both receptors and from mice striatum.

在本工作中,我们通过免疫共沉淀和BRET法证明,HEK293T细胞上瞬时表达的μ阿片受体和D1多巴胺受体能形成异源二聚体,并证实μ阿片受体和D1多巴胺受体异源二聚体存在于小鼠纹状体中。

Fluorescence resonance energy transfer analysis of subunit assembly of the ASIC channel Homo-and heteromeric ASIC complexes have been verified by classical methods such as co-immunoprecipitation,or functional assays applying electrophysiology.However,the exact subunit assembly and stoichiometry of heteromeric ASIC channels in living cells are largely unknown.

用荧光共振能量转移方法研究酸敏感离子通道的装配用经典的方法如免疫共沉淀或电生理分析,认为大多数亚单位可以形成同聚体或异聚体复合物,然而关于活细胞中ASICs亚单位装配以及ASICs异聚体中各亚单位分子比的情况并不清楚。

The new method has better resolution and can be applied to larger systems than homonuclear NMR methods. Most proteins playing an important role in cells have to be studied with heteronuclear labeled samples.

异核方法的优势在于它的分辨率更好,可以研究相对较大的,同核方法研究不了的分子;对大部分在细胞中发现的意义重大的蛋白的NMR研究都必须采用异核方法。

Induction of isotypic heterostrain antibody (Ab1): BALB/c mice were immunized with C57BL/6 splenocytes to raise antibody (Ab1) between the two difference strains.

抗同种异品系抗体(Ab1)的诱导 C57BL/6小鼠作为移植供体,BALB/c小鼠作为受体。取C57BL/6小鼠的脾细胞免疫BALB/c小鼠,制备抗同种异品系抗体(Ab1)。

Results In one-month-course group,metachromatin of pericytes and endothelial-cell nucleus got together and were kept to sides,and membrane disks of rod cells were obscure with space slightly enlarged.

结果病程 1 月组,周细胞和内皮细胞核异染色质边集,视杆细胞膜盘模糊不清,间隙略有扩大;病程 3 月组,上述改变加重,毛细血管扩张、基底膜增厚,视杆细胞核固缩、异染色质浓集;病程 6 月组,上述改变更加严重,但毛细血管由扩张转为狭窄、变形,甚至闭塞。

METHODS: We measured levels of blood hydroperoxides as an indexof oxidative injury of cellular components, as well as plasmaferric-reducing ability as an index of total antioxidant potential,during sigmoidectomy under four conditions: open sigmoidectomywith sevoflurane anesthesia, laparoscopic sigmoidectomy withsevoflurane anesthesia, open sigmoidectomy with propofol anesthesia,and laparoscopic sigmoidectomy with propofol anesthesia.

在四种不同麻醉方法下乙状结肠切除术中,测定血液中的过氧化氢水平作为细胞氧化损伤的指标,以及血浆铁还原能力作为总抗氧化潜力的指标:七氟醚麻醉开腹乙状结肠切除术,七氟醚麻醉腹腔镜下乙状结肠切除术,异丙酚麻醉开腹乙状结肠切除术,异丙酚麻醉腹腔镜下乙状结肠切除术。

Drug intervention groups received either daily inhalation of budesonide, ipratropine or heparin respectively, starting on the 8th day or TGF-β1 monoclonal antibody(TB21)0.5 mg twice (6th and 19th day) via the tail veinous injection.

其它各药物干预组于制作模型第8 d起分别雾化吸入布地奈德(布地奈德组,12只)、溴化异丙托品(溴化异丙托品组,12只)和肝素(肝素组,6只)溶液。4周后检测小支气管平滑肌及胶原厚度,用免疫组化法及原位杂交法观察各生长因子在支气管肺内的表达,用放免法检测血清和BALF中细胞外基质成分Ⅲ型前胶原、层粘连蛋白及透明质酸。

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