异种细胞的

It is concluded that (1) there are no relationships between fusion rate and relativeness of the recipient cytoplasm to nucleus donor cells,(2) cytoplast of the goat MII oocyte can support the preimplantation development of SCNT embryos reconstructed with nucleus from other species,(3) the blastocyst rate of close relative inter-species SCNT embryos is higher than that of distant relative inter-species SCNT embryos.

由此结果得出以下结论：(1)山羊M II期卵母细胞胞质与供核细胞之间的亲缘性不影响两者的融合率；(2)山羊M II期卵母细胞的胞质能支持异种间体细胞核移植胚的着床前发育；(3)亲缘关系近的种间核移植胚的囊胚发育率高于亲缘关系远的种间核移植胚的。

Changdong" showed a noticeable enhancive effect when it was co-administrated with other anti-tumor agents or when it was co-applied with radiotherapy. The action mechanism study showed that an apparent cytotoxic effect of "Changdong" on ten tumor strains in vitro with a quantity-effect relationship was observed. Immunity experiment showed that "Changdong" could induce proliferation of spleen lymphocyte in mice bearing tumor and could improve NK cell" s activity, whereas has no impact on IL-2" s reproduction. It was also noticed that "Changdong" could induce human liver cancer QGY" s apoptosis in vitro, and it happened mainly on G0-G1 phase.

结果："长动"对小鼠移植瘤模型和人体肿瘤裸鼠异种移植均有明显抑制作用，量效关系明显："长动"与放疗和化疗分别联合应用有明显的增效作用：作用机制研究结果：细胞毒性试验研究表明"长动"在体外对10株肿瘤细胞有明显的细胞毒性作用，量效关系明显；免疫学试验表明"长动"可以诱导荷瘤小鼠的脾淋巴细胞增殖，增强NK细胞的活性，对IL-2的产生无明显影响；"长动"在体外可明显诱导人体肝癌QGY细胞凋亡，使细胞阻滞于G0-G1期。

Five models were used to investigate the "Changdong"" s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong"" s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result:"Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship.

使用小鼠S180肉瘤、小鼠肝癌H22实体瘤和小鼠Lewis肺癌模型，以及人体肝癌QGY和人体肠癌LOVO裸鼠异种移植模型，进行"长动"的抗肿瘤药效学研究：用小鼠肝癌H22进行"长动"合并放疗和化疗的增效作用研究；用MTT法进行"长动"对肝癌QGY、肠癌LOVO、肺癌A549、乳腺癌MCF-7、小鼠白血病P388等10株人体和动物肿瘤细胞的体外细胞毒性作用研究；用淋巴细胞转化试验、小鼠NK细胞活性试验、小鼠胸腺细胞增殖法分别测定"长动"对荷Lewis肺癌小鼠的脾淋巴细胞增殖的影响、自然杀伤细胞活性的影响和对小鼠产生白介素-2(IL-2)的影响；用流式细胞仪PI单染色法进行"长动"对人体肝癌QGY细胞的体外诱导凋亡作用和对肿瘤细胞周期的影响的研究。

On the basis of observation on the denucleation process of mammalian erythroid cells in vito and in vitro, the present study was designed to investigate the regulatory effect and its mechanism of mammalian erythroblast cytoplasmic factor on malignant phenotype and gene expression of hybrid cells formed by cellular engineering fechnique of cell hybridization between the mouse plasmocytomas SP (2/0) cell line and rat erythroblasts.

本研究选用正常大鼠中幼、晚幼成红细胞与小鼠浆细胞瘤（SP2/0）细胞进行异种细胞杂交，研究哺乳动物红细胞胞质因子对肿瘤细胞恶性调控的效应及其机制。

Hollow fiber reactor is investigated mostly in the research of BAL owing to shortage of homogeneous hepatocytes, Security hidden danger of liver cell lines and unclear study results of hepatic stem cell.

由于同种肝细胞、肝细胞株和肝干细胞分别存在来源困难、安全隐患和研究欠深入等问题，故培养异种肝细胞的中空纤维反应器成为BAL研究的主要方向。

Objective To evaluate the metabolic activity of adult xenogeneic animal hepatocytes to bilirubin after resuscitation from liquefacient nitrogen cryopreservation of patients with severe hepatitis.

目的 评价成年异种动物肝细胞液氮冻存復苏后对人重型肝炎胆红素的代谢活性，探討生物人工肝支持系统使用冻存復苏异种肝细胞的实际价值。

After contact with xeno-target cells, the cytotoxicity of natural killer cells and the secretion of cytokines are related to the ratio of effecter cells and target cells.

NK细胞与异种靶细胞接触后，其杀伤活性和细胞因子的分泌水平与效靶细胞的比例在一定范围内呈正相关。

A new method for enucleating MⅡ nucleus in mouse and rat was induced; Living births were obtained from IVF of mouse oocytes reconstructed by transfer of metaphase Ⅱ chromosomes. For the interspecies nuclear transfer between mouse and rabbit, the reconstructed embryos could develop into blasocyst in vitro.

在不同品系小鼠卵母细胞核质交换移植中将电融合的重构卵进行IVF，经胚胎移植后顺利产仔；在异种核移植方面，小鼠核与兔卵母细胞的异种重构胚在体外培养条件下发育至囊胚阶段。

A significant time-and-concentration-dependent inhibition of cell growth was found in all the cells treated with berbamine.

利用建立的人白血病裸鼠异种移植模型，在体外与体内研究小檗胺对多种类型白血病细胞的作用，明确小檗胺是否能诱导多种白血病细胞凋亡以及多种凋亡相关基因在其诱导细胞凋亡中的作用，为小檗胺有可能开发成为新型的抗白血病药物，并应用于临床治疗，提供实验和理论依据。

Macrophages have been identified as the predominant cell within rejecting xenografts and were shown to be capable of destroying xenografts.

已有实验通过间接手段证实，巨噬细胞在异种移植的免疫反应中起效应细胞的作用，破坏移植物。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。