异种组织

Objective To investigate bio characteristics of bone stromal cells in different concentrations of alginate combined with xenograft.

目的 探讨不同浓度的海藻酸盐凝胶与去抗原异种骨构建组织工程载体的生物学性能。

In this paper, callus culture, derived from leaf or petal, and capitulum culture combined with irradiation of γ-ray for mutation were introduced.

菊花有30%品种为变异种，且菊花组织培养之分化与逆分化非常容易调控，因此本文介绍利用叶片或花托癒伤组织、或花托培养等方法，配合γ-射线照射进行诱变育种。

So study on bone tissue engineering are becoming one of the hot points in the field of Orthopaedic research.

而机体产生的排异反应，疾病传播的可能性和骨不愈合等并发症又使异种骨移植的应用受到限制，所以对组织工程化骨组织的研究成为目前骨科研究的热点之一。

This improved establishment xenogenic deproteinizated bone scaffold immunogenicity is lower, do not affect autogeneic MSCs ossify, and if it compound with rhBMP2, its ossify ability equivalency with autogenous bone.

改良法制备异种脱蛋白骨能够作为组织工程支架修复山羊胫骨大段骨缺损； 4。

That first, these are organized by the animal transplanting often is able to be discriminated against by human being's immunity system; Secondly, these animal self's a little virus is in the cards meeting be infected by to human being body inner; After once again, last phase dies because xenogenesis organ transplants since in 1990's, the more and more few people is ready to attempt xenogenesis organ transplanting.

首先，这些被移植的动物组织经常会被人类的免疫系统排斥；其次，这些动物自身的一些病毒有可能会传染到人类体内；再次，自从在20世纪90年代末期因异种器官移植而死亡后，越来越少的人愿意尝试异种器官移植。

Methods The specimens which were identified as osteosarcoma were cultured primarily in vitro. The surviving cells underwent morphological observation, histochemical staining, isoenzyme pedigree determination, cell cycling analysis, karyotype analysis and heterotransplantation.

采用原代组织块培养法对成骨肉瘤手术标本进行培养，对存活细胞进行形态学观察、组织化学染色、乳酸脱氢酶同工酶谱、细胞周期检查、核型分析、异种移植。

The experimental model of the spinal fusion in the lumbar intertransverse process was produced in 24 male goats aged 6-8 months, which were divided into 3 groups according to different implant graft.

动物骨均有相似的生物材料结构和造型，关键问题是植入体内免疫问题［2］。以往许多研究证明采用脱蛋白方法可达到消除异种骨免疫原性目的，这暗示着异种骨可能会是一种经济、有效的组织工程骨支架材料。

The experimental model of the spinal fusion in the lumbar intertransverse process was produced in 24 male goats aged 6-8 months, which were divided into 3 groups according to different implant graft.

动物骨均有相似的生物材料结构和造型，关键问题是植入体内免疫问题［2］。以往许多研究证明采用脱蛋白方法可达到消除异种骨免疫原性目的，暗示着异种骨可能会是一种经济、有效的组织工程骨支架材料。

Methods MSCs were cultured with inbred line small-ear cancellous bone of Ban Na pigs . A 1.5 centimeter segmental defect was created in the mid-upper part of the radial shaft of adult rabbit. The defects were implanted into composited xenogeneic bone in experimental group and xenogeneic bone alone in control group , the defects were not filled with anything in blank group . The repair capability of the defects was assessed by scan electron microscope before operation and physical , histology, X-ray , transmission electron microscope, SPECT and bone mineral density examinations 4 , 8 and 12 weeks after operation .

将MSCs与版纳近交系小耳猪松质骨在体外联合培养，兔桡骨中上段制成1.5cm的骨—骨膜缺损模型，实验组植入复合异种骨，对照组植入单纯异种骨，空白对照组不植入任何材料，分别于术前行标本的扫描电镜观察和术后4周，8周，12周各时间点行标本的大体观察，组织学观察，X线片观察，透射电镜观察，SPECT扫描及骨密度测试，比较其骨缺损区骨修复愈合情况。

To provide basic parameters of evaluating the biological safety of xenotransplantation from pig to human, ear tissues from 31 individuals were randomly collected from a Shazi Ling pig population. PCR and RT-PCR were performed to detect porcine endogenous retrovirus proviral DNA and mRNA respectively. The sensitivity of the PCR was evaluated using a positive control.

为评价从猪到人异种移植的生物安全性提供依据，从湖南沙子岭猪的保种群内随机采集31头个体的耳样组织，应用PCR和RT-PCR技术分别检测这些组织中内源性逆转录病毒（porcine endogenous retrovirus，PERV）的前病毒DNA和mRNA，并对PCR扩增的灵敏性进行评估。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。