异种移植的

However, there is some defects in the properties of DAF, such as its limited decay-accelerating activity and lacking of cofactor activity of I factor, which limited its usage in clinic.

然而， DAF仍存在某些缺陷，比如其有限的衰变加速能力并缺乏Ⅰ因子的辅因子活性限制了DAF的临床应用，其为部分病原微生物受体的角色对异种移植有潜在危险。因而对DAF进行改造是一种较好的选择，也是当务之急。

It is concluded that (1) there are no relationships between fusion rate and relativeness of the recipient cytoplasm to nucleus donor cells,(2) cytoplast of the goat MII oocyte can support the preimplantation development of SCNT embryos reconstructed with nucleus from other species,(3) the blastocyst rate of close relative inter-species SCNT embryos is higher than that of distant relative inter-species SCNT embryos.

由此结果得出以下结论：(1)山羊M II期卵母细胞胞质与供核细胞之间的亲缘性不影响两者的融合率；(2)山羊M II期卵母细胞的胞质能支持异种间体细胞核移植胚的着床前发育；(3)亲缘关系近的种间核移植胚的囊胚发育率高于亲缘关系远的种间核移植胚的。

Five models were used to investigate the "Changdong"" s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong"" s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result:"Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship.

使用小鼠S180肉瘤、小鼠肝癌H22实体瘤和小鼠Lewis肺癌模型，以及人体肝癌QGY和人体肠癌LOVO裸鼠异种移植模型，进行"长动"的抗肿瘤药效学研究：用小鼠肝癌H22进行"长动"合并放疗和化疗的增效作用研究；用MTT法进行"长动"对肝癌QGY、肠癌LOVO、肺癌A549、乳腺癌MCF-7、小鼠白血病P388等10株人体和动物肿瘤细胞的体外细胞毒性作用研究；用淋巴细胞转化试验、小鼠NK细胞活性试验、小鼠胸腺细胞增殖法分别测定"长动"对荷Lewis肺癌小鼠的脾淋巴细胞增殖的影响、自然杀伤细胞活性的影响和对小鼠产生白介素-2(IL-2)的影响；用流式细胞仪PI单染色法进行"长动"对人体肝癌QGY细胞的体外诱导凋亡作用和对肿瘤细胞周期的影响的研究。

So study on bone tissue engineering are becoming one of the hot points in the field of Orthopaedic research.

而机体产生的排异反应，疾病传播的可能性和骨不愈合等并发症又使异种骨移植的应用受到限制，所以对组织工程化骨组织的研究成为目前骨科研究的热点之一。

These observations show that reprogramming is easier in interspecific embryos reconstructed with ES cells than that reconstructed with somatic cells, and that ES cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells. Oocytes were reconstructed with outbreeding Kunming albino mouse ES cells and enucleated rabbit oocytes, and the effects of the passages of ES cells and 6-DMAP on the development of interspecific reconstructed oocytes were analyzed. The interspecific reconstructed ES-rabbit oocytes were activated either by combined two set electric pulses and 6-DMAP or by two set electr

以上结果显示，6－DMAP能增加胚胎干细胞异种重构卵的卵裂率，对重构卵囊胚的发育率影响不大；高培养代数和低培养代数的胚胎干细胞用于异种核移植不影响异种重构卵的卵裂率和囊胚发育率；用胚胎干细胞作为供核细胞；比体细胞作为供核细胞所构建的异种重构胚更容易进行重编程，并且胚胎于细胞指导异种克隆胚胎正常发育的能力强于体细胞。

The preimplantation development competences of somatic cell nuclear transfer embryos reconstructed with enuleated goat Metaphase II oocytes matured in vivo and whole cells derived from adult fibroblasts of several mammalian species (goat, boer goat, bovine, tahr, panda) and human patient were evaluated.

研究去核山羊体内成熟的M II期卵母细胞与异种成年的哺乳动物(包括山羊、波尔山羊、牛、塔尔羊、熊猫)及人的成纤维细胞融合形成的体细胞核移植胚胎着床前的发育能力。

Studies of molecular genetics have demonstrated that retinoblastoma susceptibility gene is closely related to the development of retinoblastoma and the theory of Rb gene mutation or inactivation has been putatively proven to be the molecular mechanism of RB formation.

本研究以我国培养建立的一株RB细胞系〓及裸鼠为实验材料和对象，在对〓细胞抑癌基因（Rb基因、p53基因）表达状况进行检测和鉴定的基础上，通过建立裸鼠玻璃体腔RB常位异种移植模型，构建Rb基因的逆转录病毒表达载体，以脂质体介导的Rb基因转移方式，进行RB体内基因治疗的实验研究，观察Rb基因在体内对RB的治疗作用及影响因素，并探讨其作用机制。

Objective To develop a new small-caliber vascular xenograft and evaluate the feasibility of xenogenic artery for coronary artery bypass grafting.

目的 研制和评价一种新型小口径异种血管，为冠状动脉旁路移植术提供新的移植血管来源。

The present study has demonstrated the pattern of TNF-α and IL-6 expression, has defined the effect of cellular immunity on BMP-induced osteogenesis at the implant site, and has clarified the immunoregulation by BMP subsequent to bone xenografting, thus opening up a new field of molecular immunology associated with bone xenograft, allowing for the search for a more ideal approach to preventing immune rejection and developing a highly effective xenogenous graft material.

本研究结果揭示了异种骨移植局部TNF-α和IL-6的定位表达及其规律，基本上阐明了移植局部细胞免疫对BMP诱导成骨作用的影响及BMP在异种骨移植局部的免疫调节作用等问题，开辟了异种骨移植分子免疫学新领域，并为选择更理想的移植排斥防治途径和研制高效的异种骨移植材料提供了可靠的实验及理论依据。

A new method for enucleating MⅡ nucleus in mouse and rat was induced; Living births were obtained from IVF of mouse oocytes reconstructed by transfer of metaphase Ⅱ chromosomes. For the interspecies nuclear transfer between mouse and rabbit, the reconstructed embryos could develop into blasocyst in vitro.

在不同品系小鼠卵母细胞核质交换移植中将电融合的重构卵进行IVF，经胚胎移植后顺利产仔；在异种核移植方面，小鼠核与兔卵母细胞的异种重构胚在体外培养条件下发育至囊胚阶段。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。