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To make the wapiti sperm capacitated in vitro, we selected and used the method of sperm-up. By making the rate of acrosome reaction as a main index, and the sperm energy, the survial time and the fertilizated rate of wapiti sperm and hamster mature oocytes as auxiliary indexes, it were carried out to compare the effects of wapiti sperm capacitation in vitro under different temperature, different density of pyruvate sodium, caffeine and heparin and different culture medium.

对马鹿精子,使用上浮法诱导其获能,通过对顶体染色测定精子的顶体反应率并作为主要指标,同时以精子活力、精子存活时间和异种受精率为辅助指标,对精子的获能效果进行评价,以比较不同培养温度、丙酮酸钠浓度、咖啡因浓度、肝素浓度以及培养基对马鹿精子体外获能效果的影响。

Three types of cells were transferred into perivitelline space under zona pellucida of rabbit oocytes respectively.

用体外培养14代和24代的小鼠胚胎干细胞构建的异种重构卵的卵裂率分别是88.5%和82.1%(P>0.05),囊胚发育率分别是16.7%和15.4%(P>0.05)。

The research work can make us acquire the further recognition of molecular mechanism about blastocyte implantation and parturition and provide the theoretical basis for some leading-edge problems, such as organ transplantation, homeoclone or heteroclone. In the meantime, the result might be used as possible targets of medicine development for the cure of some reproductive diseases, such as sterility, abortion and fetal malformation.

研究这些关键过程将使我们对胚泡植入和分娩的分子机理获得进一步的认识,同时也可为器官移植、同种或异种克隆等研究热点提供基础理论依据,在临床上也为治疗一些妊娠疾病如不孕、流产、胎儿畸形等提供药物可能作用的靶位点。

Objective: To observe the attachment and growth of fibroblasts on a new porcine collagenic dermal matrix and investigate a method to improve the cell compatibility of the porcine dermal scaffold.

目的:观察成纤维细胞在一种新型的猪胶原基真皮支架上的粘附生长特性,探讨提高异种真皮支架细胞相容性的方法。

A series of technologies were established and applied to this study, which include MR electron microscopy by using mannosylated albumin-colloidal gold (DMA-G10) as probe, modified CBB method for acrosome reaction detecting, improved model of human acrosome reaction induced by mZPS (solubilized mouse zona pellucida), in vitro heterogenic fertilization and affinity cytochemistry, preparation and purification of proteins.

本研究建立了MR的电子显微镜探针——甘露糖基化白蛋白-胶体金及其检测法、移植建立了人精子顶体反应的CBB检测法、改良了mZPS诱导人精子顶体反应实验模型,同时运用体外获能、异种体外受精、亲和细胞化学、蛋白质提纯制备等技术与实验模型,对人精子MR的表达、定位和生理功能加以探讨。

One way to increase the heterologous production is to increase the pool of precursors in the host.

这种异种生产是依赖于高效表达系统,为类胡萝卜素的基因簇,但增加的供应量前体在宿主生物体,是具有重要意义的。

Methods The in vitro cultured porcine vascular endothelial cells and human sera were co-reacted to set up an in vitro model of hyperacute rejection of pig-to-human xenotransplantation.

用体外培养的猪血管内皮细胞和不同人的血清共同反应,建立猪与人异种移植超急性排斥反应的体外实验模型。

In contrast, there are truncated proviral sequences that have the capacity to retrotranspose, causing insertional mutagenesis in the xenograft and in infected human cells.

相比较而言,被截短的前病毒序列有逆转录转座作用,能够导致异种移植物以及被感染的人类细胞的插入突变。

Posterolateral Lumbar Intertransverse Process Spinal Arthrodesis With Composites Composed Of Recombinant Human Bone Morphogenetic Protein 2 And Allograft Or Xenograft; Or With Composite Consisted Of HA And Xenograft in rabbitsBackground: So far spine arthrodesis remains to be used in spine surgery.

中文题名rhBMP-2、HA及其复合物在兔腰椎后外侧融合的实验研究副题名外文题名论文作者杨发军导师马忠泰教授学科专业外科学研究领域\研究方向临床技能训练与研究学位级别博士学位授予单位北京大学学位授予日期2001 论文页码总数92页关键词骨形态发生蛋白异体骨移植脊柱融合异种骨移植馆藏号BSLW /2001 /R687 /42 背景:目前,脊柱融合手术被广泛地应用于脊柱外科领域,自体髂骨仍是被最常用的骨移植物,但是其有5%-35%的不融合率。

It is simple and effective. Currently, the reconstruction materials include autologous, allograft, xenograft, synthesized nonabsorbable and absorbable patches, and acellular patch.

目前的重建材料有自体组织、同种异体移植物、异种移植物、合成的不可吸收补片、合成的可吸收补片、脱细胞补片等,各有其优缺点。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。