异种免疫的

It is conceivable that the breaking of immune tolerance of MMP-2 should be a useful approach to cancer therapy by active immunity.

我们设想通过主动免疫的方法，用异种MMP-2 DNA疫苗打破对自身MMP-2的免疫耐受，可能达到治疗肿瘤的目的。

To study the mechanism of the immune tolerance of the xenogenic epikeratophakia,4 patients with keratoconus and 5 patients with aphakia were treated by epikeratophakia monkey lenticules.

摘 要 目的：研究异种表面角膜镜片术后机体对异种角膜的免疫反应，阐明人体对异种角膜的免疫耐受机制。

These observations may provide a vaccine strategy for cancer therapy through the induction of the autoimmunity against the molecules associated with tumor growth in a cross-reaction with a single xenogeneic homologous gene and may be of importance in the further exploration of the applications of other xenogeneic homologous genes identified in human and other animal genome sequence projects in cancer therapy.

这些结果提示，通过单个异种同源基因以交叉免疫的方式打破肿瘤生长相关分子的自体免疫耐受是一种新的肿瘤疫苗治疗途径，并显示出进一步探索人类和其他种属的异种同源基因在肿瘤免疫治疗应用中的重要性。

That first, these are organized by the animal transplanting often is able to be discriminated against by human being's immunity system; Secondly, these animal self's a little virus is in the cards meeting be infected by to human being body inner; After once again, last phase dies because xenogenesis organ transplants since in 1990's, the more and more few people is ready to attempt xenogenesis organ transplanting.

首先，这些被移植的动物组织经常会被人类的免疫系统排斥；其次，这些动物自身的一些病毒有可能会传染到人类体内；再次，自从在20世纪90年代末期因异种器官移植而死亡后，越来越少的人愿意尝试异种器官移植。

To observe analgesit effects of xenogeneic bovine chromaffin cells and protective effects of the alginate-polylysine-alginate microcapsule.

采用微囊化牛嗜铬细胞异种移植于大鼠和癌痛病人脊髓蛛网膜下方法，观察BCC镇痛作用和海藻酸钠-聚赖氨酸-海藻酸钠微囊对异种移植物的免疫保护作用。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

The experimental model of the spinal fusion in the lumbar intertransverse process was produced in 24 male goats aged 6-8 months, which were divided into 3 groups according to different implant graft.

动物骨均有相似的生物材料结构和造型，关键问题是植入体内免疫问题［2］。以往许多研究证明采用脱蛋白方法可达到消除异种骨免疫原性目的，这暗示着异种骨可能会是一种经济、有效的组织工程骨支架材料。

The experimental model of the spinal fusion in the lumbar intertransverse process was produced in 24 male goats aged 6-8 months, which were divided into 3 groups according to different implant graft.

动物骨均有相似的生物材料结构和造型，关键问题是植入体内免疫问题［2］。以往许多研究证明采用脱蛋白方法可达到消除异种骨免疫原性目的，暗示着异种骨可能会是一种经济、有效的组织工程骨支架材料。

The present study has demonstrated the pattern of TNF-α and IL-6 expression, has defined the effect of cellular immunity on BMP-induced osteogenesis at the implant site, and has clarified the immunoregulation by BMP subsequent to bone xenografting, thus opening up a new field of molecular immunology associated with bone xenograft, allowing for the search for a more ideal approach to preventing immune rejection and developing a highly effective xenogenous graft material.

本研究结果揭示了异种骨移植局部TNF-α和IL-6的定位表达及其规律，基本上阐明了移植局部细胞免疫对BMP诱导成骨作用的影响及BMP在异种骨移植局部的免疫调节作用等问题，开辟了异种骨移植分子免疫学新领域，并为选择更理想的移植排斥防治途径和研制高效的异种骨移植材料提供了可靠的实验及理论依据。

Macrophages have been identified as the predominant cell within rejecting xenografts and were shown to be capable of destroying xenografts.

已有实验通过间接手段证实，巨噬细胞在异种移植的免疫反应中起效应细胞的作用，破坏移植物。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了