异源的

The research progress on synthetic allopolyploid including type, inducement method and utilization status in crop genetic improvement were summarized, and the feasibility and importance for using it to improve crop varieties were discussed. Finally, the existing problems in relevant researches were analyzed, and the future studies were prospected.

概述了近年来植物人工异源多倍体的研究进展，包括人工异源多倍体的类型、诱导方法及其在植物遗传改良中的应用现状，探讨了利用人工异源多倍体进行农作物品种遗传改良的可行性和重要性，并对相关研究中的问题进行了分析和展望。

Studies on sequence changes in the early genomes of interspecific F_1 hybrid and amphidiploid in CucumisIn the present study,extensive genomic changes were detected in newly obtained allotetraploid of Cucumis,namely Cucumis hytivus Chen and Kirkbride,by AFLP analysis using 12 pairs of EcoR-I/Mse-I primers.

甜瓜属远缘杂种F_1及人工异源双二倍体早期基因组序列变化研究利用12对Eco RI/Mse I选择性引物，对甜瓜属人工异源双二倍体C.hytivus及其二倍体父母本进行AFLP分析，结果发现多倍体基因组在形成早期发生了广泛的序列变化。

F〓 of transgenic allotetraploid fish containing"all-fish"transgene——pCAgcGHc was obtained respectively by inbreeding and gynogenesis. Positive rate of pCAgcGHc detected by PCR was 90％ in F〓 of transgenic allotetraploid fish produced by inbreeding, while most F〓 of transgenic allotetraploid fish produced by gynogenesis showed diploidy measured by flow cytometer. The results above imply that developing the pure line of transgenic allotetraploid fish through inbreeding is feasible, but the operation should be modified if gynogenesis is introduced.

采用近交和雌核发育方法培育出转pCAgcGHc基因异源四倍体鲫鲤F〓代，通过PCR检测，外源基因pCAgcGHc在近交转pCAgcGHc基因异源四倍体鲫鲤F〓中的整合率为90％，而流式细胞技术检测雌核发育转pCAgcGHc基因异源四倍体鲫鲤F〓的倍性多为二倍体，说明通过近交筛选转基因异源四倍体鲫鲤纯系的方法是可行的，但是通过雌核发育来筛选转基因异源四倍体鲫鲤纯系还要就方法上进一步的改进。

Diploid gynogenesis was induced by activating egg development with UV irradiated sperm (80 mJ/cm2, the homologous or the heterogenous sperm) for 5 min in seawater at 18 ℃, and then subjecting the eggs to cold shock in 3 ℃ seawater for 45 min.

无论同源精子还是异源精子，最佳诱导条件为在18 ℃条件下，精子经80 mJ/cm2的紫外线照射，受精后4~5 min将受精卵放在3 ℃海水中进行冷休克处理，持续时间为45 min，同源精子和异源精子二倍体诱导分别为32.66%±7.03%和28.00%±6.48%。

Objective To compare the effectiveness of heteroduplex, SSCP and heteroduplexSSCP analyses in mutational screening.

目的比较异源双链、单链构像多态、异源双链SSCP法筛查突变的效率，寻找简便有效的基因突变筛查方法。

However, the titration and protein concentration of IBDV in vero cells was higher than that in CEF cells.

因此选用异源的咖细胞大量增殖IBDV，并采用不同的方法进行纯化比较。

In present study, we demonstrated that μ opioid receptor and D1 dopamine receptor could form heteromeric complexes in membranes preparation from HEK293T cells transiently cotransfected with both receptors and from mice striatum.

在本工作中，我们通过免疫共沉淀和BRET法证明，HEK293T细胞上瞬时表达的μ阿片受体和D1多巴胺受体能形成异源二聚体，并证实μ阿片受体和D1多巴胺受体异源二聚体存在于小鼠纹状体中。

However, PAPC alone is not sufficient to induce an ectopic otic placode formation.

同时，研究发现PAPC异位注射并不足以诱导一个新的异源的耳基板的形成。

Thus, the meiotic chromosome cofigurations showed that the tetraploids and hexaploids in C.reticulata allopolyploids, and the appearance of a few tetravalents in some populations might indicatehomology of chromosomes in different genomes to some extent.

根据减数分裂的构型，我们认为，四倍体和六倍体分别为异源四倍体和异源六倍体，少数四价体的存在表明染色体有部分同源性。

The seed set frequencies ranged from 0.001% to 0.184%.The chromosome number of F2 plant root tip varied from 39 to 56.4 In the Fa progeny of wheat *AR7l, Some plants showed higher alien translocation frenquencies.

在小麦与黑麦AR71属间杂种的F_2中，个别植株发生了较高的异源易位频率，其中有1株(229-3植株)产生了多达3个异源易位。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。