异氰酸

ShTat1.3 and ShTat1.5 are two variable antigen types of a cloning Anhui strain of Trypanosoma evansi.Total RNA of the two variable antigen types were extracted by acid guanidinium thiocyanate-phenol-chlorform single step method.

应用异硫氰酸胍一酚一氯仿一步法分离伊氏锥虫安徽株克隆虫体ShTatl的二个抗原变异体ShTat1.3和ShTat1.5总RNA，在含有甲醛的琼脂糖凝胶电泳后，转印到硝酸纤维膜上。

Methods The template DNA was extract from the human osteosarcoma cells line U－2OS by the single- step isolation method with isothiocyanic acid guanidine, the cDNA coding for the mature fragment of BMP－4 was amplified by the reverse transcription- polymerase chain reaction.

方法采用异硫氰酸胍一步法从人骨肉瘤细胞株U-2OS中提取总RNA，通过逆转录-聚合酶链反应扩增为BMP-4成熟区cDNA基因片段。

METHODS: A series of N-octyl-N'-succinyl chitosan labeled with fluorescein, were incubated with HepG2 liver cancer cells, K562 leukemia cells, A549 human lung cancer cells, and BGC cancer cells respectively and followed by using flow cytometry and enzyme-linked immunosorbent assay to assess the affinity and inhibition of N-octyl-N'-succinyl chitosan for the four kinds of tumor cells already mentioned.

用异硫氰酸荧光素对N-正辛基－N'-琥珀酰基壳聚糖进行标记，再分别与人肝癌细胞(HepG2)、人白血病细胞(K562)、人非小细胞肺癌细胞(A549)及人胃癌细胞共培养，通过流式细胞仪及酶联免疫检测仪测定N-正辛基－N'-琥珀酰基壳聚糖对肿瘤细胞的亲和性及抑制力。

Isothiocyanates are a class of important organic synthetic intermediates, and have been used extensively in the synthesis of substituted thiourea pesticides.

异硫氰酸酯是一种重要的有机合成中间体，在合成取代硫脲类农药中有广泛应用。

Effects of four insecticides on intestinal mucosa permeability of the wolf spider were studied by exposing spiders to insecticides and then measuring the concentration of fluorescein isothiocyanate-dextran in the spiders'blood. The results showed that FITC-D concentrations of the test groups were much more than that of the control group.

采用异硫氰酸荧光素-葡聚糖作为示踪物质，通过检测拟环纹豹蛛血液内的荧光素浓度，研究了两种生物源杀虫剂和两种化学源杀虫剂对拟环纹豹蛛肠黏膜通透性的影响。

An improved guanidine thiocyanate method has been developed for total RNA extraction from the leaves and roots of the succulent xerophyte Zygophyllum xanthoxylum which contains high levels of polyphenolics and polysaccharides. In order to remove polyphenolics interference, 4%β-mercaptoethanol was added in the extraction buffer.

针对多浆旱生植物霸王富含多酚和多糖类物质的特点，在常规异硫氰酸胍方法的基础上进行改进，摸索出一种霸王叶和根总RNA提取的方法。

RT-PCR amplification of ginseng β-amyrin synthase gene was successfully performed based on the total RNAs extracted from ginseng hairy roots induced by Agrobacterium rhizogenes A4 using a modified guanidine isothiocyanate-method.

以发根农杆菌A4菌株诱导的人参发根为材料，用改良的异硫氰酸胍法提取总RNA，得到了纯度好的完整的总RNA。

Total RNA is isolated from rice caryopses displaying different development status by using the methods of TRIZOL Regent, Guanidine Thiocyanate and improved SDS.

利用TRIZOL试剂快速提取法、异硫氰酸胍法和改良的SDS法提取不同发育时期水稻颖果的总RNA，并通过凝胶电泳、紫外分光光度法检测提取获得的RNA样品的品质。

Extraction of high-quality RNA from Panax ginseng tissues is particularly difficult due to high levels of polyphenolics and polysaccharides, or other compounds that bind and/or coprecipitate with RNA. The methods such as guanidinum thiocyanate, CTAB and modified Trizol were applied to isolate high purity and integrity RNA from Panax ginseng.

针对人参组织多酚、多糖类物质含量较高的特点，比较了改良的异硫氰酸胍法、改良的CTAB法和改良的Trizol法等3种不同的RNA提取方法。3种改良的方法均能从人参组织中提取到总RNA。

Ester of poisonous armour of different sulfur cyanic acid, formaldehyde and vulcanization hydrogen are decomposed to wait in soil.

在土壤中分解成有毒的异硫氰酸甲酯、甲醛和硫化氢等。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。