异核性

F〓 of transgenic allotetraploid fish containing"all-fish"transgene——pCAgcGHc was obtained respectively by inbreeding and gynogenesis. Positive rate of pCAgcGHc detected by PCR was 90％ in F〓 of transgenic allotetraploid fish produced by inbreeding, while most F〓 of transgenic allotetraploid fish produced by gynogenesis showed diploidy measured by flow cytometer. The results above imply that developing the pure line of transgenic allotetraploid fish through inbreeding is feasible, but the operation should be modified if gynogenesis is introduced.

采用近交和雌核发育方法培育出转pCAgcGHc基因异源四倍体鲫鲤F〓代，通过PCR检测，外源基因pCAgcGHc在近交转pCAgcGHc基因异源四倍体鲫鲤F〓中的整合率为90％，而流式细胞技术检测雌核发育转pCAgcGHc基因异源四倍体鲫鲤F〓的倍性多为二倍体，说明通过近交筛选转基因异源四倍体鲫鲤纯系的方法是可行的，但是通过雌核发育来筛选转基因异源四倍体鲫鲤纯系还要就方法上进一步的改进。

The main objective of this project is to research and develop a novel and high efficient video codec framework based on wavelet techniques, especially to address the heterogeneousness of video networks, the fluctuation of transmission bandwidth and the diversity of video-stream receivers. The system will have some basic characteristics of the next video coding standard, such as high compression efficiency, high scalability and high error resiliency. This project will provide a software prototype of wavelet video codec with full scalability, construct a verification platform for wavelet video on demand service, and furthermore, design some VLSI IP cores of the key module in the codec system

本项目研究的主要目的就是着眼于下一代视频编解码标准的基本特征（高效压缩，高可伸缩，高容错），以小波技术为核心构建新型高效的视频编解码系统；特别针对当前视频传输网络的异构性，网络带宽的波动性以及用户视频接收终端的多样性，实现一个码流高度可伸缩同时兼顾压缩效率的小波视频编解码软件原型，并且构建基于异构IP网络的小波视频服务的技术验证平台；进一步还将基于VLSI技术，对小波视频编解码系统的关键模块进行IP核设计

The results show that the primers S37 and S61 are of apparently specific to the amplified bands of six strains, which establishes the basic for the degenerate molacular marker of Cordyceps militeris and confirms heterokaryosis and parasexuality of Cordyceps militeris.

结果表明，引物S37、S61对6个菌株的扩增谱带具有非常明显的特异性，该结果为蛹虫草退化分子标记奠定基础，并且在一定程度上也印证了蛹虫草异核现象和准性生殖理论。

The spiny eel , belonging to Perciformes, Osteichthyes, is a kind of little freshwater fish distributed in Southern China. In 1998, Our lab detected XX/XY differentiated sex chromosomes in this specie with karyotypic analysis, and then with chromosomal binding and SC observation, we identified that its two largest metacentric chromosomes were X, Y sex chromosomes in 1999. X, Y chromosomes differ each other not only in the distribution of heterochromatin but also in their shapes, which make them could be recognized directly under the light-microscope without any banding treatment.

刺鳅，属鲈形目、刺鳅科(Perciformes，Mastacembelidae)，是广泛分布于中国南方的小型淡水鱼类。1998年，我室通过核型分析，首次发现该物种具有明显分化的异形性染色体，其性别决定类型为XX／XY型。1999年，本室通过染色体显带、精母细胞联会复合体观察，再次确认刺鳅染色体组中最大的一对中部着丝粒染色体为X、Y性染色体，两者不仅已经出现结构异染色质分布上的不同，同时在外部形态上也存在明显差异，识别特征鲜明，在光镜观察下无需显带即能清晰辨认性染色体。

On light microscopy,there was abundant cosinophilic cytoplasm,with the tumor cells being aciniform or tubiform without significant atupia and mitosis.

光镜下瘤细胞排列成腺泡状或管状，细胞质含丰富的嗜酸性细颗粒，细胞无明显异形性和核分裂象。

Based on the results of this report and the results of literature reports on the preferred conformation of enantiomeric 3-substituted-1, 4-benzodiazepines, a nucleophilically solvated C3 carbonium ion intermediate resulting from either a P or a M conformation is proposed to be an intermediate.

基於本文所报导的结果及文献所记述之对映异构性在C3位有取代基时的1，4-苯并二氮类的倾向构象，设想提出所观察之现象是由正或负构象所致的一个亲核性经溶剂化的C3阳碳离子为中间体。

Among the fish which were cytogenetically studied, most of them do not possess any morphologically differentiated sex chromosomes. On the other hand, karyotypic analysis, chromosome binding, synaptonemal complex observation and comparative genomic hybridization show in few fish species, that there exist sex chromosomes which differentiated at different levels and types.

在进行过细胞遗传学研究的鱼类中，绝大部分没有发现异形性染色体，然而通过核型分析、染色体显带、联会复合体观察和比较基因组杂交等方法，发现在少部分鱼类中确实存在有异形分化的性染色体，并且分化程度高低不等，性别决定方式多种多样。

Primary cultured chondrocytes are poly-angle, cytoplasm-rich, and their nuclei are either round or oval with clear necleole. Metachromatic and alcian blue positive staining in primary cultured chondrocytes was observed. Intercellular matrix was anti-collagen type Ⅱ staining but not anti-collagen type Ⅰ staining by IHC assay.

原代培养软骨细胞呈多角型，胞质丰富，胞核成圆形或椭圆型，核仁清楚，甲苯胺蓝呈异染性，阿尔新蓝8Gx 染色阳性，细胞外基质Ⅱ型胶原免疫组化染色阳性，Ⅰ型胶原染色阴性。

Four male sterile lines of different nuclei genotypes with the same cytoplasm of Aeglops bicornis ,msbicorj-83 (37 65. ms-yanshi 9^ ms-80 (6) and ms-90-110, were selected as the materials in the study on gene location of B -type cytoplasmic male sterile lines by using monisomic analysis and nullisomic -tetrasomic analysis methods.

本研究选用4个同质异核二角型不育系ms-83(37)65、ms-偃师9号、ms-80(6)及ms-90-110为材料，采用单体和缺四体两种方法对二角型不育系进行了育性基因定位。

As exposed to optics microscope and inverted microscope,compared to control,SW480 cells with Giemsa staining that treated with the H2RA(cimetidine、nizatidine) took on malignance declining as crimpled firstly,cellular bulk and heteromorphism dimimished,the amount of rounded cell manifolded,nucleolus bulk lessened,dyeing thin,the amount of nucleolus decreased,partial cell organs manifest side-gathering phenomenon on chromatin,and the link between SW480 cell is loosened , subsequently partial rounded cell is floated.When the H2RA(cimetidine 40μM、nizatidine 20μM) against SW480 cells after 3 day,SW480 cells change roundly,nucleolus smashed,shrinking nucleolus came into being,and part of those not adhibited cell wall.

在光镜和倒置相差显微镜下，与对照组相比，经姬姆萨染色观察，西咪替丁、尼扎替丁处理的SW480细胞，先发生皱缩，体积变小，异形性减少，细胞变圆数目增多，细胞核变小，染色变淡，核仁数量减少，部分可见染色质边集现象；细胞间连接疏松，随后部分变圆细胞浮起。40μM西咪替丁及20μM尼扎替丁连续处理3天后，SW480细胞全部变圆，细胞核碎裂，固缩微核形成，部分不再贴壁。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。