异构化作用

In the thesis alkylate and bridging resorcarenes were used as CGC SP. Their chromatographic character were studied.

将烷基化和桥联的间苯二酚杯芳烃用作气相色谱固定相，考察了色谱性能，对普通化合物表现出良好的分离能力，尤其是对芳香族位置异构体有良好的分离作用。

1H NMR spectroscopic analysis of aromatic protons combined with theoretical analysis of molecular structures proved the existence of two rotational isomers at 300 K, as well as a rapid interconversion equilibrium at 330 K for both compounds Ⅰ and Ⅱ. However, only one conformer exists for compound Ⅲ and puerarin containing 7-phenolic hydroxyl instead of propyl in A-ring as the case of compounds Ⅰ and Ⅱ. Based on UV-Visible absorption data of neutral and basic solutions, and on the density function calculations, the 7-phenolic hydroxyl group in A-ring was found to be more acidic than the 4'-phenolic hydroxyl group in B-ring. The mechanism of derivation reaction and the structure-reactivity relationship of puerarin as an antioxidant were further discussed.

其中7-丙基葛根素Ⅱ属于新型取代衍生物；变温1H NMR和理论计算结果表明，在300 K时，葛根素和4'-丙基葛根素以单一构型存在；而7，4'-二丙基葛根素和7-丙基葛根素则存在两种核磁可分辨的旋转异构体，当温度升至330 K时，两种异构体可以相互转化；通过对比分析葛根素及其衍生物脱质子产物的紫外可见吸收光谱并结合理论计算，确定了A环上7位酚羟基比B环上4'位酚羟基先脱去质子，并由此进一步阐明了葛根素衍生化反应机理以及在抗氧化作用中可能的活性位点和结构活性关系。

The data transformation platform is designed in accordance with modularization, which masks the access from upper application by data wrapping module and submits DataSet objects to upper application. DataSet exchange module finishes heterogeneous data exchange by using XML capsulation of DataSet. For the synchronization process of heterogeneous data, it uses table trigger and Updates Information Table to capture change from source database table. The Update Information Table keeps the DML statements operated on tables which need to be synchronized. In the course of synchronization, synchronization module extracts DML operation field from Regenerate Information Table DataSet. At last, it updates the table fields of destination database by using command object.

数据转换平台的实现采用模块化设计，通过数据包装模块屏蔽上层应用对数据库的访问和将包装好的数据集对象提交给上层应用来实现，数据转换模块利用DataSet对XML的封装完成底层异构数据的交换；在异构数据同步的处理上，采用触发器结合更新信息表的方式获取源数据库上表内容的变化，更新信息表保存作用在同步表上的DML语句，然后在同步的过程中利用数据转换平台的同步模块对更新信息数据集中的DML操作字段进行数据抽取，最后通过命令对象将变化更新到目的数据库的表字段中。

The crystal structure analysis of photocolored product showed that the photochromic phenomenon was due to photoisomerization from enol form to keto form.

结构分析表明：其光致变色现象是由于它在溶剂与光的共同作用下发生了从醇式到酮式的异构化。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

It may have a preventional function from Alzheimer's disease. Molecular biological studies are shown that apoE isoform has a isoform-specific role to neurodegeneration. ApoE〓 and apoE〓 aid to sustain the stabilization of cytoskeleton protein tau, while apoE〓 hyperphosphorylates tau to destabilize the microtubule protein, which will make neurocyte easy to form neurofibrillary tangles in AD. Clearance of amyloidal peptide by apoE〓 and apoE〓 is more effective than by apoE〓. ApoE〓 has the strongest ability to clear the amyloidal peptide. It is shown that apoE〓 has a stronger prevention from Alzheimer's disease.

分子生物学研究发现，apoE异构体对神经细胞退化表现出异构体特异作用，apoE〓和apoE〓有助于维持微管的稳定性，而apoE〓则使tau蛋白超磷酸化，破坏微管的稳定性，使神经细胞易于形成神经纤维缠结。apoE〓和apoE〓对A β的清除率远远高于apoE〓，apoE〓对A β的清除能力最强，可见apoE〓对AD的发生具有较强的阻止作用。

The first step in the pathway of bilin biosynthesis is the conversion of heme to biliverdin IX by the heme oxygenase encoded by ho1, then PCB:ferredoxin oxidoreductase encoded by pcyA catalyze BVD to 3Z-PCB.

在血红素氧化酶的作用下，血红素Ⅸ被氧化而共轭环断裂形成胆绿素IV，胆绿素IV被藻蓝胆素合成酶还原和异构化形成藻蓝胆素PCB。

Characteristics of Inclusions: After the tea is kept for a long period of time in a stall and deep fermentation processing begins, the specialties of material and manufacturing make many bioreactions occur for thorough activation of microbes to excrete extra-cellular enzymes, which impacts over 500 stuffs of tea. Theses microbes contain catechin, polyphenols, amylase, cellulose, protein, lipid ,alcohol, ketone, vitamin, microorganism, and organic acid, making them transform, isomerize and degrade, polymerize and couple, so as to produce amylase, thearubigins, theaflavins, cellulose, and microelements that other commercial teas could not produce or produce too little.

三、 内含物的特点：由于原料、制造的特别性，经长期陈化和深发酵的过程，茶叶内含物发生多种生物生化反应，充分激活微生物分泌胞外酶，作用于茶叶中的内含儿茶素、多酚、多糖、纤维素、植物素、蛋白质、脂类、醇类、酮类、维生素、微量元素、有机酸等500多种物质，使之转化、异构降解聚合耦联，生成其他商品茶无法比拟和缺乏的多糖、茶红素、茶黄素、纤维素、微量元素等，并形成以菇稀醇类为主体香气。

The main gluconeogenic precursor in kidney is thought to be lactate; however, less is emphasized enantiomerically. L-lactate is a glycolysis end-product, but D-lactate is formed after detoxification of methylglyoxal, which is the main source of advanced glycation end-products.

乳酸为肾脏糖质新生的主要来源，其含有一不对称碳，故具有D-、L-乳酸两种镜相异构物，而D-、L-乳酸两者之生成相当不同，L-乳酸是糖解作用之终产物，D-乳酸为体内一醣化终产物(advanced glycation end-products)─甲基乙二醛进行去毒化反应所生成，目前缺乏对乳酸镜像异构物与肾脏糖质新生间相关的探讨。

ERp57 isomerizes specific interchain disulfides connecting the major capsid protein, VP1, to a crosslinked network of neighbors, thus uncoupling about 12 of 72 VP1 pentamers.

ERp57能使特异的链键二硫键发生异构化，使主要衣壳蛋白VP1与邻近的蛋白发生网络交联，从而使72个VP1五聚体中12个发生解耦连作用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。