异基因的

The mating type gene is inherit decision factor of compatibility with the performance type,P.ostreatus belongs to sexual reproduction heterothallism,tetrapolarity,Namely the colony which is consist of single spore mycelium will self-sterility,If conjugate sex gene with all for incompatibility,then crossing can dicaryotization and form sporophore.

交配型基因是性亲和表现型遗传决定因子，平菇为有性生殖异宗配合四极性类型，即单孢形成的菌落自体不育，不同单核菌丝在配对基因皆为杂交结合时才能双核化，形成子实体。

Objective To compare the effectiveness of heteroduplex, SSCP and heteroduplexSSCP analyses in mutational screening.

目的比较异源双链、单链构像多态、异源双链SSCP法筛查突变的效率，寻找简便有效的基因突变筛查方法。

SW480 and SW620 cells were also used to study the gene regulation with high carbohydrate intake, where it was shown that during glycolysis eight genes, HK1 (nuclear gene encoding mitochondrial protein, transcript variant 1), GPI, GAPD (glyceraldehyde-3-phosphate dehydrogenase), PGK1 (phosphoglycerate kinase 1), PGK2 (phosphoglycerate kinase 2), ENO2 (enolase 2), PKM2 (pyruvate kinase, muscle, transcript variant 2) and GLUT1 (facilitated glucose transporter, member 1) are over-expressed. In our study, we also found that during hypoxia of cancer tissues the resulting up-regulation of HIF-2α(hypoxia-inducible factor 2, alpha subunit) would in turn up-regulate the GLU1 gene, further activating glycolysis.

另一方面，在高碳水化合物的调控研究上，吾人也利用SW480和SW620细胞株分析证实醣解水解活化过程中，其六碳醣激酶-1(HK1)、葡萄糖磷酸异构酶、3-磷酸甘油醛脱氢酶、磷酸甘油酸激酶-1(PGK1)、磷酸甘油酸激酶-2(PGK2)、烯醇酶-2(ENO2)、丙酮酸激酶-2(PKM2)以及葡萄糖输送蛋白-1(GLUT1)等共有8个基因是连续上升的过度表现；此外，吾人也发现当癌细胞在缺氧的组织中，其缺氧转录子-2α基因(HIF-2α)表现上升后，会活化GLU1基因上升，进一步活化醣解代谢的进行。

Respectively use chelex 100 resinate,boiling extraction,proteinase K digest/isopropanol precipitation,proteinase K digest/phenol/chloroformextra ction,alkaline lysis,guanidine thiocyanate lysis,guanidine hydrochloride lysis,spin column method to extract Mycobacterium tuberculosis DNA for multiplepolymerase chain eraction.

选择3份含结核分枝杆菌分别为4.325×103、6.857×104、5.356×105 Copies/ml的痰液，分别用Chelex 100树脂法、煮沸法、蛋白酶K消化异丙醇沉淀法、蛋白酶K消化酚/氯仿抽提法、碱裂解法、异硫氰酸胍裂解法、盐酸胍裂解法、离心柱法抽提结核分枝杆菌DNA作多重聚合酶链反应，显色法芯片检测结核分枝杆菌RFP和INH耐药基因。

AIM: To investigate the alteration of gene expression profile of thalamus in rats anesthetized with propofol using gene chip.

目的：运用基因芯片研究异丙酚麻醉大鼠丘脑基因表达谱的变化。

AIM摘要: To investigate the alteration of gene expression profile of thalamus in rats anesthetized with propofol using gene chip.

目的摘要：运用基因芯片探究异丙酚麻醉大鼠丘脑基因表达谱的变化。

It is similar in structure to αandβ-Asarone that are genetoxic to rat hepatocytes. Carpacin''s isomer, myristicin is not genetoxic to rat hepatocytes and can induce glutathion S-transferase activity that is reported to have effect in rat hepatocytes.

其类似物α与β-Asarone对鼠肝细胞具有基因毒性及其异构物myristicin对鼠肝细胞并无基因毒性，且可诱发出glutathion S-transferase等解毒酵素的活性。

By using overlap extension PCR,a P137G site mutant named xylA137 was introduced in the xylose isomerase gene xylA from Thermus thermphilus.

利用重叠延伸法对嗜热细菌的木糖异构酶基因xylA进行体外P137G定点突变，通过酿酒酵母表达载体XM204将突变基因xylA137引入酿酒酵母H158中，得到重组菌株H158-XI137。

According to pmi gene sequence in genebank, the 6-phosphomarmose isomerase gene of Escherichia coli was amplified by PCR.

根据genebank中公布的pmi基因序列设计引物，通过PCR扩增从大肠杆菌中分离出6-磷酸甘露糖异构酶基因。

For this, the main research content of the theme is as follows: based on the cultivation and drug-sensitive test of standard isolates and clinical separate isolates of tubercle bacilli, to develop a multi-PCR-SSCP detection technique for aphC promoter.

对H37Rv标准株和临床分离株分别采用常规P＊R和 nutiPCR同时进行扩增，结果两种扩增方法均能扩增出预期的目的片段，符合率达 100％。二、单基因SSCP检测耐异烟朕基因突变 1。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。