异基因

BACKGROUND: Allogeneic hematopoietic stem cell transplantation is recognized as the only method of curing chronic myelocytic leukemia.

背景：异基因造血干细胞移植是目前公认的惟一可以治愈慢性粒细胞白血病的方法，但存在供体来源少，移植相关并发症较多等问题。

To investigate the high risk elements analysis of chronic graft versus host disease.Explore early diagnosis of late-onset noninfectious pulmonary complications.

研究目的慢性移植物抗宿主病是异基因造血干细胞移植后的主要并发症，探讨慢性移植物抗宿主病发病的高危因素，探讨迟发性非感染性肺部并发症的早期诊断，为临床有效防治cGVHD提供指导依据。

Then transplantation of the sciatic nerve from donor rats to the recipient rats in two groups were progressed.7 days after nerve transplantation,the contents of cd4 and cd8 in peripheral plasma of recipient rats were detected by flow cytometry.8 weeks after nerve transplantation,transplanted nerve were observed under light microscope.results:compared with nonirradiated group,the percentage of cd4 and the ratio of cd4 to cd8 in uvb irradiated group were significantly decreased（p＜0.05,p＜0.01）,cd8 percentage was significantly increased（p＜0.01）.conclusion:uvb-irradiated donor splenocytes were injected in the recipient rats through caudal veil,which could induce immunological tolerance to allogeneic transplantation.

未照射组注射未经紫外线照射的供体脾细胞，照射组注射经紫外线照射的脾细胞，7 d后移植供体神经。术后7 d行外周血t淋巴细胞亚群检查，术后8 w行移植神经组织学检查。结果：照射组cd4+百分率、cd4+/cd8+比值较非照射组明显降低（p.01，p.05），cd8+百分率较非照射组明显升高（p.01）。组织学检查显示，照射组的再生神经较非照射组多。结论：尾静脉内注射经紫外线照射的异基因脾细胞可诱导对异体神经移植的特异性免疫耐受。紫外线；脾细胞；免疫耐受；坐骨神经

Allogeneic stem cell transplantation is the main treatment of choice for many hematological malignancies, as well as some nonmalignant hematological disorders, but disease relapse is still the most significant obstacle to allo-HSCT.

异基因造血干细胞移植是目前许多恶性血液病和一些非恶性疾病的主要治疗手段，但疾病的复发仍是影响allo-HSCT效果的主要原因。

Objective To explore the key technique of allogeneic whole pancreaticoduodenal transplantation in rats.

目的 探讨异基因大鼠全胰十二指肠移植模型手术过程的操作要点。

Additional 5 Balb/c and 5 B6 mice were implanted peritoneally with hybridoma cell-containing diffuse chamber.

目的 观察自制细胞扩散匣的免疫隔离作用及匣内异基因瘤细胞的在体生长情况。

Murine MSCs were migrated to the immune organs of rats with xenogenic liver transplantation and showed inhibitory effect on lymphocyte proliferation.

小鼠MSCs可迁徙至异基因肝移植大鼠免疫器官内，并具有抑制淋巴细胞增殖的作用。

D. The function of stimulating xenogenous lymphocyte proliferation was the same between peripheral DCs and ascites PCs. E. The percentage of CD3〓CD56〓 cells was the same in CIK cells co-culture with DCs transfected with SKOV3 RNA, CIK cells co-culture with DCs, and CIK cells. F. The expansion rate of CIK cells can be accelerated by co-culturing with loaded or unloaded DCs. However, the expansion rate between loaded or unloaded group is the same. F. The strongest cytotoxicity against SKOV3 cell line was achieved by CIK cells co-cultured with DCs loaded with SKOV3 lysate.

结果：1、腹水可获得0.83±0.24×10〓个AMC/ml，单核细胞有0.74±0.25×10〓个/ml；2、卵巢癌患者外周血可获得0.87±0.20×10〓个AMC/ml，单核细胞有0.92±0.17×10〓个/ml；3、除CD86外周血单核细胞来源DC表达较高以外，其他表面分子在不同来源DC间没有统计学差异；4、不同来源DC的异基因刺激能力没有差异；5、与负载或未负载卵巢癌抗原的DC共培养并不能提高CIK细胞群中CD3〓CD56〓细胞的数量；6、CIK细胞增殖显著，培养14天时可扩增19.18±4.70倍，培养21天时可扩增35.82±4.36倍；7、与未负载或负载DC共培养的CIK细胞在培养第14天后增殖速率大于单纯CIK细胞。

The objective of this study was to investigate the function and mechanism of hyperbaric oxygen in antagonizing acute graft-versus-host disease and improving the rate of survival.

本研究探讨高压氧在抗异基因骨髓移植后aGVHD和提高生存率中的作用及机制。

The objective of this study was to investigate the function and mechanism of hyperbaric oxygenin antagonizing acute graft-versus-host diseaseand improving the rate of survival.

本研究探讨高压氧在抗异基因骨髓移植后aGVHD和提高生存率中的作用及机制。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失