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There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group).The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group).The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组(K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组),采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量,流式细胞术检测细胞内阿霉素的浓度,基因、酶学、蛋白水平检测实验分3组(K562组、K562/A02组和K562/A02+TTD组),采用RT-PCR法检测mdr1 mRNA的表达,免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平,Western-blotting法检测P-糖蛋白和bcl-2表达。

Than cross-pollinated plants, which harbor more genetic variability.

参考译文:由于持续的同种繁殖,同株授粉的植物比异株授粉的植物表现得更具一致性,从而使基因突变可能性更大。

There were statistically significant differences in favor of developing tophi in the heterozygote TNFα-308G/A p=0.017, odds ratio

甲型肿瘤坏死因子促进因子基因-308位置之异合子会加重痛风石的产生(p=0.015, odds ratio 6.11, 95% C.I.1.22-30.49)。

In the study, RT-PCR and sequencing were employed to detect the expression levels of CCK-B receptor, CCKBRi4sv and gastrin mRNAs in 30 human gastric carcinoma and their corresponding normal tissues, 10 gastritis and 2 autopsied normal stomach specimens as well as in a gastric carcinoma cell line SGC-7901 cells.

本研究首先用RT-PCR及测序方法检测了30例人类胃癌组织及癌周正常胃粘膜、10例胃炎、2例尸检胃组织及胃癌细胞株SGC-7901中CCK-B受体、CCK-B受体的异位剪接变异体(intron 4-contained variant of cholecystokinin B receptor,CCKBRi4sv)及胃泌素基因的表达。

The Promega commercial DNA extraction kit was used as control. Results The Promega kit resulted in a higher copy number of COWP gene[(6.45-9.86)×106]than that of Generay commercial DNA kit [(2.38-3.69)×106 ], 5% guanidine thiocyanate[(1.27-21.29)×105] or 2% Triton X-100 [(2.06-866.70)×103], respectively.

结果 Promega试剂盒的裂解液裂解隐孢子虫卵囊的效果最好,纯化所得卵囊COWP基因拷贝数可达(6.45~9.86)×106;其他依次为捷瑞试剂盒的裂解液[(2.38~3.69)×106]、5%异硫氰酸胍[(1.27~21.29)×105]和2% Triton X-100[(2.06~866.70)×103]。

In this thesis, using maize of same nucleus but different cytoplasms and same cytoplasm but different nuclei as the experimental materials, RNA editing of mitochondrial atp6 and cox2 gene in different tissues was analyzed.

本文以同核异质、同质异核48-2、黄早四两组玉米为材料,对不同器官组织线粒体的功能基因atp6、cox2进行了RNA编辑分析。

The homeobox gene is in close relation to develop...

结论同源异形框基因的差异表达表明正常成人和胎儿皮肤的发育生物学状态存在差异,可能是胎儿和成人皮肤不同创面愈合方式的根本原因。

Some important issues, such as the identification of synonyms and homonyms, the classification of germplasm resources, the breeding with sexual cross and early selection, the discovery and evaluation of mutants, and even the localization and cloning of important genes are all important research subjects to be addressed.

例如,荔枝品种中同物异名与同名异物情况的区分、种质资源的鉴别与合理的分类、杂交育种与早期选择、突变的发掘与鉴定评价、甚至重要性状基因的定位与克隆等,都是很需要解决的研究问题。

Yeast ; Protein disulfide isomerase; Gene expression

酵母;蛋白二硫化物异构酶;基因表达

It was conjectured that the protein expressed by the gene could he enzyme, isomerase or intermediate metabolite and it had relation with the function of insulin receptor.

推测该基因表达的蛋白质可能是酶、异构酶或者中间代谢产物,与胰岛素受体的作用有关。

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推荐网络例句

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。