异基因

Collectively, subjects with the NAT2 fast acetylator genotype were more prone to CRC and reflected the possibility that exposure to HCAs may contribute to CRC development in Taiwan, especially in Taiwanese women.

总之，NAT2之基因多型性之分析结果发现，台湾人具有NAT2 快速代谢基因型者较容易罹患大肠直肠癌。并可推测异环胺类（heterocyclic amines， HCAs）之暴露可能参与台湾大肠直肠癌之形成，特别是台湾妇女族群。

Here we summarize the progress of salmon calcitonin's heterogenous expression in prokaryotic and eukaryotic expression systems as well as the amidation of C-terminal. Both the biological activity of salmon calcitonin and the biologic expression system can be improved by genetic engineering techniques.

本文综述了鲑鱼降钙素基因在大肠杆菌、链霉菌、乳酸杆菌和蓝藻等原核细胞，在酵母、昆虫、动植物等真核细胞中异源表达的研究进展，以及通过基因工程的方法能够改进生物表达系统，提高鲑鱼降钙素活性的动态。

It is similar in structure to αandβ-Asarone that are genetoxic to rat hepatocytes. Carpacin''s isomer, myristicin is not genetoxic to rat hepatocytes and can induce glutathion S-transferase activity that is reported to have effect in rat hepatocytes.

其类似物α与β-Asarone对鼠肝细胞具有基因毒性及其异构物myristicin对鼠肝细胞并无基因毒性，且可诱发出glutathion S-transferase等解毒酵素的活性。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

This thesis mainly focus on the Enzyme histochemistry and TEM observation of trunk muscle from Branchiostoma belcheri tsingtauense; isolation and recombinant expression of Bbt-myosin heavy chain isoforms; characterization and expression of Bbt-nascent-polypeptide-associated complexαsubunit.In NADH Enzyme histochemistry, two types of muscle lamella can be distinguished .

本论文主要对青岛文昌鱼(Branchiostoma belcheri tsingtauense)肌肉组织进行了酶组织化学实验和电镜观察，分离了青岛文昌鱼肌球蛋白重链基因异构体并将其重组表达；克隆了青岛文昌鱼新生肽链相关复合体α亚基基因，并检测了其时空表达情况。

The results of HPLC tests indicated that Bt toxin in Bt transgenic cotton had not obviously affected the contents of flavonoids (mainly rutin and isoquercitrin and a few quercetin ),contradictively ,the result of combined dosage test in vitro showed that that rutin had significantly enhanced the suppression of Bt-ICP on the cotton bollworm larvae., in addition to the

采用高效液相色谱法测定转Bt基因棉花主要抗虫黄酮类化合物的的变化，结果表明外源Bt杀虫蛋白表达不会对棉花自身原有的主要抗虫黄酮类物质（包括芸香苷、异槲皮苷和含量较少的槲皮素）产生不利的影响，因此可以将Bt杀虫蛋白基因转入抗虫黄酮类物质中国农业科学院博士学位论文含量高的棉花品系。

We aim to screen and identify the key potential trans-factors during hemoglobin switch. We firstly analyzed differential expression of mRNAs in erythroid induction cultures of CD34+ cells derived from normal umbilical cord blood, adult bone marrow, and bone marrow of a heterocellular hereditary persistence of fetal hemoglobin patient. We identified ZF (HCF-binding transcription factor, Zhangfei) and SH3GLB1(SH3-domain GRB2-like endophilin B1) that had differential expression in the above three cultures. Furthermore, we confirmed the different expression of the above two genes by quantitative real-time PCR.

为鉴别影响珠蛋白基因表达和开关的新的重要调节因子，我们首先分析了人脐带血、正常成人骨髓和异细胞型胎儿血红蛋白持续存在综合症患者骨髓细胞红系诱导培养物中基因表达的差异，发现ZF(HCF-binding transcription factor，Zhangfei)和SH3GLB1（SH3-domain GRB2-like endophilin B1）在这三种不同来源的红细胞内具有显著的表达差异，并通过实时定量PCR方法验证了差异的真实性。

First, miRNAs derive from genomic loci distinct from other recognized genes, whereas siRNAs often derive from mRNAs, transposons, viruses, or heterochromatic DNA.

第一，微小RNA 起源于明显不同于其他识别基因的基因位点，而小型干扰RNA通常起源于信使RNA、转座子、病毒或异染色质的 DNA。

In this study 2mG2-epsps gene was used to be a selection marker gene, and a series of experiments were designed for determining the time of their screening and selection pressure. The results showed that 1 mM glyphosate isopropylamine and 15 days screening can be effectively inhibited non-transgenic maize callus tissue differentiation. So this research work has provided a safe and effective screening system for corn and other monocotyledons crops.

实验中以2mG2-epsps基因作为筛选标记基因，为确定其筛选时间及筛选压力，进行了玉米愈伤组织对不同浓度草甘膦的抗性实验，确定向培养基中添加浓度为1mM的草甘膦异丙胺盐筛选15天能有效的抑制非转基因玉米愈伤组织的分化，本研究的再生植株的阳性率为45%，为玉米及其它单子叶作物转化提供了一种有效、安全的筛选体系。

To analyze and predict the physical and chemical characteristics of Apis mellifera triosephosphate isomerase,several intergrated bioinformatics databases and local programs were used frequently.

运用生物信息学的方法对蜜蜂丙糖磷酸异构酶基因及其编码蛋白序列进行了初步分析，得到了与基因和蛋白质组学研究相关的一些基本参数。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失