异基因

Discussions are given of the fate of Ms2 during translocation in the hexoploid triticale, the exchange of the names for 4A and 4B chromosomes in common wheat and the possible expoitation of the new locus Ms2 (4BS), and the following speculations are made: In geneic genes of allopolyploid organisms the donor chromosomes tend to be intergenomically translocated to their phylogically and evolutionarily close chromosomes with the same order number and the same arm; it is cofirmed that the 7th International Conference of Wheat Genetics was right to exchange the names between chromosomes 4A and 4B of common wheat in 1988; and as a new genetic marker and a breeding tool for all the chromosome B-carrying species in the tribe of Tritceae, Ms2 (4BS) may have wide application in building and expanding the gene pool of germplasm resources of various species of wheat.

对 Ms2基因在六倍体小黑麦与原太谷核不育小麦远缘杂交中易位时的走向，普通小麦4A与4B染色体的互换更名以及 Ms2(4BS)新位点的开发利用进行了讨论：认为异源多倍体生物核基因的组间易位倾向于从供体染色体向进化亲缘关系较密切，且染色体序数与染色体臂相同的部分同源染色体易位；1988年第7届国际小麦遗传学会对普通小麦4A与4B染色体的互换更名是正确的； Ms2(4BS)作为一个新型的遗传标记，作为小麦族内所有携带B染色体组的物种的育种工具和在拓建各类小麦种质资源的基因库等方面均有广泛的用途。

The genomic organization differences at both gene junctions provide new evidence of extremely divergence between Heterotremata and Thoracotremata in brachyuran crabs.

两个基因连接部的基因组成差异为短尾类内部异孔类与胸孔类之间的歧异提供了新的证据。

Chinaberry witches'broom, Ziziphus jujube witches'broom, Mulberry dwarf disease, JWB and Paulownia witches' broom phytoplasmas were identified by comparative studies of PCR amplify of phytoplasmal 16SrDNA、23rDNA and ribosomal protein gene, heteroduplex mobility assay, RFLP of PCR-amplified products (16S rDNA), cloning and sequence analysis of 16SrDNA and rp gene. An efficient procedure for rapid identification and differentiation of phytoplasmas was established, which could identify and differentiate phytoplasma collected from field.

通过对桑萎缩病、枣疯病、酸枣丛枝病、泡桐丛枝病和苦楝丛枝病植原体16SrDNA、23SrDNA和核糖体蛋白基因的PCR扩增、异源双链迁移率分析、16SrDNA PCR产物的限制性片段多态性分析以及植原体16SrDNA和rp基因的克隆和序列分析等比较研究，建立了一种快速确定未知植原体种类和分类地位的分子鉴别与鉴定优化程序；此程序可对田间采集的各种植物植原体样品直接进行快速鉴定和鉴别。

The results indicated that the expression level of cgi-100 decreased in K562 cells treated with matrine. Heterochromatin decreased, euchromatin and the proportion of S phase in K562-cgi-100 cells increased, and cell proliferation enhanced.

结果表明，在苦参碱作用K562细胞后cgi-100基因表达下调；重组K562细胞中cgi-100基因过表达，且常染色质增加，异染色质减少，细胞增殖旺盛，S期细胞增多，增殖速度高于对照组。

Respectively use chelex 100 resinate,boiling extraction,proteinase K digest/isopropanol precipitation,proteinase K digest/phenol/chloroformextra ction,alkaline lysis,guanidine thiocyanate lysis,guanidine hydrochloride lysis,spin column method to extract Mycobacterium tuberculosis DNA for multiplepolymerase chain eraction.

选择3份含结核分枝杆菌分别为4.325×103、6.857×104、5.356×105 Copies/ml的痰液，分别用Chelex 100树脂法、煮沸法、蛋白酶K消化异丙醇沉淀法、蛋白酶K消化酚/氯仿抽提法、碱裂解法、异硫氰酸胍裂解法、盐酸胍裂解法、离心柱法抽提结核分枝杆菌DNA作多重聚合酶链反应，显色法芯片检测结核分枝杆菌RFP和INH耐药基因。

Results The levels of HSP 70 mRNA and HSP 70 protein expression were significantly different among the 5 groups. Ketamine induced marked HSP 70 mRNA and HSP 70 protein expression in the posterior cingulated cortex. Propofol itself did not induce HSP 70 gene expression in this brain region. Propofol significantly inhibited ketamine-induced HSP 70 mRNA and HSP 70 protein expression in the posterior cingulate cortex in a dose-dependent manner.

结果氯胺酮可明显诱导HSP70 mRNA与HSP70蛋白在大鼠后扣带回皮质区的表达；异丙酚自身不能诱导HSP70基因的表达；预先给予异丙酚可显著抑制氯胺酮诱导的HSP70 mRNA和HSP70蛋白在这一区域的表达，且抑制效应呈剂量依赖性。

Lines which were 2n=21"+1" and one line which was 2n=20"+2", one line which was 2n=43. The salt-resistance addition line Line15 was confirmed as a Disomic alien addition line by C-banding, SSR, FISH methods, and the two alien addition chromosomes had close correlation with the long arm of the second homoeologous group, the centromere region of the third homoeologous group and the short arm of the seventh homoeologous group. We also confirmed the particular C-banding type of the alien addition chromosomes by C-banding following FISH method. The drought-resistance addition line Line17 was found also as a Disomic alien addition line by SSR and FISH detection. The alien addition chromosomes had close correlation with the short arm of the second homoeologous group.

对导入赖草优质耐盐基因的株系Line15进行了C-分带、荧光原位杂交、SSR相结合的鉴定，确认Line15是一个二体异附加系，附加的一对染色体与小麦的第二同源群长臂、第三同源群着丝粒和第七同源群短臂密切相关，并确定了Line15的外源染色体C-分带特征带型，为目后鉴定Line15附加系的真伪提供了简便快捷的途径；对抗旱附加系Line17进行了荧光原位杂交和SSR检测，得出Line17也是一个二体异附加系，外源染色体与小麦的第四同源群短臂密切相关。

The findings were compared with those from ovarian carcinomas unrelated to endometriosis, atypical endometriosis, and ovarian endome...

结果：起源于内异症的卵巢子宫内膜样癌和卵巢透明细胞癌中各有1例检测到K-ras基因第12密码子发生突变，突变类型为GGT→TGT，即甘氨酸→半胱氨酸，其余标本均未见第12、13密码子突变。p21蛋白在与内异症相关的卵巢肿瘤。。。

After discussion of the relationship among idioblastic cells,tolerance to abiotic stress and betaine,the author thought the reason of transgenic plants having an increased number of idioblastic cells was that BADH was introduced and much betaine might be synthesized.

通过对异形细胞、植物抗逆性、甜菜碱三者之间的关系讨论，认为转基因植株异形细胞数量多是由于BADH基因的导入可能合成了较多甜菜碱。

However,ectopic expression of this gene in other mature organs caused cell or plant death,indicating a strict regulatory role of SFL in the development of flower organs in rice.

作者发现SFL在花器官中异位表达后能激活雌蕊特征基因DL的表达，从而导致花器官的同源异型转换，而在其他成熟器官中异位表达则导致细胞死亡，显示SFL在水稻花器官发育中的特异性调控功能。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失