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Objective To appraise the value on combined detection of telomerase activity,DNA aneuploid and carcinoembryonic antigen for the laboratory diagnosis of malignant pleural effusion.

目的评价联合检测胸腔积液中端粒酶活性、DNA异倍体和癌胚抗原对实验诊断恶性胸腔积液的价值。

Results For malignant diseases, the sensitivity of aneuploid is 75.44%, the specificity is 71.43% and the accuracy is 73.74%.

结果以异倍体作为指标诊断恶性胸腹水的敏感性为75.44%,特异性为71.43%,准确率为73.74%。

That PI and the percentage of the cells in s-phase of the borderline/malignant and heteroploid/aneuploid tumors were higher ted to higher mortality and relapse rate as well as the corresponding risk. Because of the changable characters and dissociative phenomenon of the tumor, the measurement of PI and the percentage of cells in s-phase may be disturbed in few of diploid tumor and in part of double DNA stem ones. Therefore other paramet- ers should be added to analyse before making any conclu- sion.

交界性和恶性、异倍体或非整倍体肿瘤的PI和S期细胞百分率较高,病死加复发率和相对危险性增加;由于肿瘤的异质性和"分离现象",对于少数2倍体肿瘤和部分双DNA干系肿瘤PI和S期细胞百分率的测定易受干扰,故应结合其它参数综合分析。

Result:The content of cholesteatoma tissues and postauricular skin specimens are a normal euploid.

结果:胆脂瘤上皮细胞和外耳道上皮细胞的核型均为二倍体,未见异倍体。

The NPC patients with DNA heteroploid are more easily to emerge distant metastases than those with DNA diploid.

采用流式细胞术检测新鲜组织细胞的DNA倍体性和临床分期一样可以预测鼻咽癌患者的预后;DNA异倍体患者比二倍体患者更容易出现远处转移而导致治疗失败。

This study shows that the quantity DNA heteroploid in precancersis with larynx increases as high as the grade does,and DNA heteroploid may be one of the important markers of precancersis with larynx.

提示喉癌前病变的DNA异倍体率随不典型增生病变的分级增高而增加,不典型增生程度越高,癌变率越高,提示DNA异倍体可能是喉癌变早期的分子标志物。

Of them are treated with immunohistochemistry modus to detect Ki-67 expression; 4. Use SPSS13.0 for windows statistics software to chisquare inspection. Results: through detecting, heteroploid pecentage and Ki-67 positive expression of the patient who suffered breast cancer grouped in the zhengqi-weak-evil-strong type is the highest in the three typies(69.4%,94.4%).

结果:乳腺癌患者术前辨证为正虚毒炽者DNA倍体异倍体、Ki-67阳性出现率最高(69.4%,94.4%),冲任失调组次之(41.0%,58.3%),肝郁痰凝组最低(23.5%,12.5%),经统计学处理,三组之间有显著差异(P.05);正虚毒炽组S期细胞比例为高度的几率最高(58.3%),冲任失调组和肝郁痰凝组无显著差异(17.9%,23.2%)。

The expression rates of CD44V5+ CD44V6+, cerbB-2+ cells in patients with metastasis, multi-metastasis nodes, DNA heteroploid, SPF elevated, and Apo decreased were higher than those of patients with non-metastasis, only one-metastasis node, DNA diploid, SPF non-elevation and Apo non-decreasing, respectively (P.01 or 0.05), while nm23+, DCC23+ and p16+ were contrary.

肿瘤转移者、多器官转移者、DNA异倍体者、SPF增高者、APO不减低者的CD44V5+、CD44V6+、cerbB-2+细胞表达率均显著高于未转移者、单器官转移者、DNA二倍体者、SPF不增高者、APO不减低者(P.01或0.05)。而nm23+、DCC+、p16+细胞的表达率与此相反。

The heteroploidy rate of PC group was 65.71%,NP group and BPH group were DNA diploid,the cell proliferous ratio of PC group was different with NP,BPH groupP

PC高分化组的异倍体率低于中、低分化组(P.05)。DNA异倍体、高S期细胞比率是前列腺癌的重要生物学特性,也可作为鉴别PC、BPH的参考指标。

Methods The exfoliated cells from 84 cases hydrothorax and ascites(including 35 cases of malignant tumor group and 49 cases of non-tumor group) were analyzed in DNA ploid by FCM, the results were compared with the method of exfoliative cytology.

方法应用流式细胞仪对84例患者(恶性肿瘤组35例,非恶性肿瘤组49例)的胸腹水脱落细胞进行DNA倍体分析;以检出异倍体为恶性肿瘤判断标准,并与胸腹水常规脱落细胞学涂片检查结果比较。

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