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异位移植

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Rat model of cryopreserved aortic and pulmonary valved homograft heterotopic transplantation was established to observe the changes of humoral and cellular immunological response and pathological changes of implanted homografts.

方 法 通过低温保存的大鼠同种异体带瓣主动脉和肺动脉异位移植的动物模型,观察同种移植后宿主体液和细胞免疫指标的变化,以及移植物的病理改变。

aim to assess relationship between the increased lfa-1expression in intestine graft and acute rejection,and inhibitory effects of fk506on the expression of lfa-1.methods heterotopic small intestine transplantation was performed on inbred sd and wistar/a rats.the rats were randomly divided into4groups:control groups;isograft group(wistar/a→wistar/a);allograft group(sd→wistar/a);allograft plus fk506group(sd→wistar/a+fk506),

目的 探讨大鼠移植小肠组织内淋巴细胞相关抗原-1(lfa-1)的异常表达与急性排斥反应之间的关系,及fk506对其的抑制作用。方法选用近交系封闭群sd和wistar/a大鼠进行全小肠异位移植。实验分4组:非手术对照组;同基因移植组(wistar/a→wistar/a);异基因移植组(sd→wistar/a);异基因移植加用fk506治疗组[sd→wistar/a+fk506

Cobra venom factor was given to prevent hyperacute rejection and FK506 was given to prevent cellular rejection. The xenograft recipients were killed and cardiac xenografts harvested 4, 8, 12, 16, 24 hours after transplantation. Histologic examination was used to demostrate the degree of rejection and coagulation while revers transcriptase-polymerase chain reaction was used to detect the expression of tissue factor mRNA, and the guinea pigs hearts, harvested but not transplanted, were studied as controls in all samples.

建立豚鼠-大鼠异种异位心脏移植动物模型,使用CVF抑制超急性排异反应,FK506抑制细胞免疫排斥反应,分别在移植术后4、8、12、16、24h切取移植心脏,通过病理切片观察排异反应和凝血强度,同时通过RT-PCR方法检测组织因子mRNA表达强度,未移植豚鼠心脏作对照。

AIM:To explore the proliferation and migration of bone mesenchymal stem cells transplanted in intervertebral disc from rabbits and the expression of exogenic gene.

王金堂,张宏,张银刚,张小卫,李萌目的:观察骨髓间质干细胞异位移植在椎间盘内的存活、迁移情况及外源基因的表达。

Objective To develop a simple allograft heart heterotopic transplantation model in the neck.

目的 建立简便易行的同种心脏异位移植动物模型。

Methods: The model of heterotopic cardiac allograft of SD to Wistar rats was established.

建立大鼠腹部心脏异位移植模型,分对照组及环孢菌素组,每组32只。

The investigators found no evidence of new collagen formation, migration, or heterotopic bone growth.

研究者并未发现新胶原蛋白的形成、移动或是异位移植骨骼生长。

This study investigated the biologic properties of artificial synthetic HAP, the osteogenous capacity of red bone marrow after being transplanted to a heterotopic site, and the worth of composite HAP-BM implanted to osteogenesis.

本文研究包括:人工合成的HAP的生物反应,自体BM异位移植的成骨和诱导成骨作用和HAP配合BM移植对成骨的价值。

Results: 48 heart transplantations were performed. We summarized surgical technique after sixteen rats of preliminary experiment. The successful rate was improved to 96.88% in 32 rats of formal transplantation. Time of cold ischemia, hot ischemia and rebeat were shorten significantly and operational time reduced to 60 min.

结果:共进行了48例大鼠心脏异位移植,经过预实验16只的技术总结,正式实验32只手术成功率为96.88%,供心冷、热缺血时间和心脏复跳时间明显缩短,手术时间减少至60min。

In control group, obturator nerve controlling right gracilis was cut off. In in situ implantation group, the right gracilis was cut off and replanted to its original site, and the obturator nerve was implanted to the muscle. In ectopic transplantation group, the right gracilis was cut off and transplanted to the muscle of the left leg, and the obturator nerve was implanted to the muscle.

对照组:切断支配右侧股薄肌的闭孔神经,造成肌肉失神经支配;原位再植组:切取右侧股薄肌,再回植于原位,闭孔神经植入肌肉;异位移植寄养组:切取右侧股薄肌,移植寄养于左侧股部,闭孔神经植入肌肉。

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