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Radiography and double fluorochrome labeling were taken. Dogs were sacrifice at 1, 3, 6 months post operation and image analysis were done on undemineralized slices then subject to statistical analysis by SAS software, Version 6. 21. 3. BMSCs in vitro induced and proliferated through complete blood culturing were seeded into tricalcium phosphate , fdDBM and 2 kinds of coralline hydroxyapatite with 200μm and 500μm porosity (C200 and C500) respectively.

将全血培养法体外诱导、扩增培养的BMSCs分别与三磷酸钙(tricalcium phosphate,TCP)、两种孔径的珊瑚羟磷灰石(200μm,500μm)以及制备的fdDBM复合,体外培养4~7日后,进行扫描电镜、组织学观察,并行裸鼠皮下回植,了解包括空白DBM材料在内的5组样本的异位成骨情况,经IMAGER-PRO PLUS软件进行图像分析,并进一步行统计分析。

Over the pillared saponite by the impulse reaction, while it was only ~4% for the pillared montmorillonite, indicating that the substitutions in the tetrahedral sheets played an important role in the development of Bronsted acidity of the catalyst.

层柱皂石的异丙苯裂解转化率达到了65%;而层柱蒙脱土的转化率只有4%。这说明层材料的四面体取代对于层柱粘土Bronsted酸位的形成具有重要的决定作用。

Abstract] objective to explore the experience in the diagnosis and treatment of small bowel diverticular surgical complication.methods 24 cases of small bowel diverticular were retrospectively analysed.results the diagnosis was made preoperatively in 4 duodenal diverticula and only 1 jejunal diverticula.clinical complications included obstruction in 11 cases,bleeding in 4 cases,perforation in 4 cases.23 cases were cured.1 case was died of mof.all the diverticular with complications had histopathological signs of inflammation and 3 had signs of ulcer and 4 had the resected.conclusion the small bowel diverticular is rare.it may diagnose except common reason underwent gastrointestinal bleeding or obstruction.the gi is an effective way of examinations.operation is effective for small bowel diverticular disease.

回顾性分析24例小肠憩室病外科并发症的临床资料。结果术前6例十二指肠憩室中4例确诊,18例空回肠憩室仅1例确诊。小肠憩室伴小肠梗阻11例,憩室出血4例,憩室穿孔4例。23例治愈,1例死于多脏器功能衰竭。有并发症的憩室病理都有炎症表现,5例有溃疡,4例有异位黏膜组织。结论小肠憩室病比较少见。在胃肠出血或梗阻的情况下,可采取对常见原因排除的方法进行诊断。在不需要急诊手术的情况下,钡餐造影是最有效的检查。手术是可靠有效的治疗方法。

Methods Immunohisto-chemical S-P method was employed to detect the expression of MMP-2in30specimens of ectopic endometrium and entopic endometrium of adenomyosis and16specimens of normal endometrium.

结果 MMP-2在子宫腺肌病异位内膜组中的表达显著高于子目的研究基质金属蛋白酶(MMP-2)在子宫腺肌病中的表达,探讨其表达与子宫腺肌病的发生及痛经的相关性。

Gliosis is resulted in TLE. The synapse consists of the presynaptic and postsynaptic terminals, which in many synapses are surrounded by glial cell.

颞叶癫痫可导致神经系统胶质增生,突触后终端与胶质细胞之间形成突触,突触后终端的树突棘的形态学的变化引起突触结构的改变,与胶质细胞之间形成的突触为异位突触,引起海马内环路性质的变化,从而提高致痫性。

The transforming growth factor-β superfamily, a large group of highly conserved growth factors including TGF-βs, activins and BMPs, regulate a wide variety of cellular functions such as proliferation, differentiation, apoptosis and migration. Signals from these growth factors are transduced by a group of Smad proteins. To date, there are nine vertebrate Smads, including the receptor-activated Smads , Smadsl-3, 5 and 8, the common mediator Smad4 and Smad4β, and the inhibitory Smads, Smad6 and 7. Signaling is initiated when the ligand induces assembly of a heteromeric complex of type Ⅱ and type Ⅰ TGFβ receptors. Then R-Smads are directly phosphorylated by activated type I TGF-β receptors.

在多种SMADS中,SMAD5令我们产生兴趣,基于三个原因:1、Smad5基因敲除胚胎中发现卵黄囊的异位造血以及CFU-GM祖细胞数目增加,表明SMAD5可能是早期造血发育的负向调控基因。;2、应用反义核酸封闭Smad5可以逆转TGF-β对祖细胞增殖的抑制效应,表明它可能介导TGF-β信号转导,后者的异常,包括某些SMADS的功能异常,和白血病紧密相关;3、Smad5基因定位在人染色体5q21,此区域的缺失和急性髓系白血病和骨髓增生异常综合症相关,因此,Smad5被怀疑为白血病抑制基因。

Micrococcal lysis assay of the milk samples showed that the cDNA was efficiently expressed in the mammary glands with similar expression levels ranging from 60 to 87 mg/L. Different tissues of each vector-injected mouse were assayed for transcription of hLYZ mRNA by dot-blotting and for expression of hLYZ activity by Micrococcal lysis assay. The results showed that expression of the three recombinant vectors was mainly restricted to mammary glands with some degrees of ectopic expression in spleen, intestines and/or kidney.

注射后72 h采集乳样,经微球菌溶解实验证明,3组小鼠乳汁中hLYZ的表达量分别为87、69、60 mg/L;从每组小鼠的12种组织提取总RNA,经Dot blotting检测证明,三种重组载体除在乳腺中表达外,p205C3LYZ还在小鼠的脾和肠、pBJLYZ在脾、pBCLYZ在脾和肾中具有一定的异位表达;在有hLYZ mRNA转录的上述脏器中用微球菌溶解试验均可检测到hLYZ活性。

In this study, isobaric tags for relative and absolute quantification labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS) was employed to compare the differential protein expressions in MCF7 cells upon 17β-oestradiol (E2) stimulation or serum deprivation versus in normal conditions. A total of 576 proteins with the confidential level of ≥95% were identified, 26 proteins among the 576 proteins were found to be differentially expressed after E2 stimulation or serum deprivation, and among them 10 proteins were up-regulated and 16 proteins were down-regulated by more than two folds.

利用四重用于相对和绝对定量的等量异位标签(isobaric tags for relative and absolute quantification, iTRAQ)标记结合二维液相色谱-串联质谱(two-dimensional liquid chromatography-tandem mass spectrometry, 2D-LC-MS/MS)对雌二醇(17β-oestradiol, E2)和血清各自以及共同作用对MCF7乳腺癌细胞内蛋白质表达的影响进行了比较,共鉴定到置信度在95%以上的蛋白质576种,其中各组相对于正常培养组的细胞共找到26种差异在1倍以上的蛋白质,其中10种上调,16种下调。

The trophoblast blood flow spectrum could be easily detected with the guide of CDE.The ultrasonic findings were characterized by broad arterial flow spectrum,high diastolic component of the low resistance trophoblastic flow,RI=0.40~0.51 with single or biphasic spectrum.The salpingian ring with high blood flow indicated early and complete salpingian pregnancy.

结果:①异位妊娠包块血流信号丰富,血管连续性好,能较完整显示血管床、血管网及范围广的低流速血流信号;②脉冲多普勒取样为滋养层周围性血流频谱,其特征为:频谱增宽、单相或双相持续存在、阻力降低(RI=0.44±0.04)、舒张期血流丰富的动脉频谱;③具有鲜明丰富的滋养层周围性彩色血流频谱特征的&输卵管环&是早期输卵管妊娠的特征性表现,并提示是输卵管完整存在的标志。

Previous studies by others and this laboratory on the allosteric effect of hem oglobins have suggested that the electrostatic replusive cavity at the center of tetrameric moiety plays a substantial role in regulating O2 transport prope rties, including chloride binding, and Bohr effect .

中文摘要过去对血红蛋白异位调节效应的研究指出,在血红蛋白四合体中央空腔的区域的静电排斥效应对分子机能的调节如氧合时氯离子的键结和波尔效应,扮演著一个很重要的角色。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。