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Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P<0.001.

结果:构建了带有HSV-tk基因的反转录病毒载体GINaTK,应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317,成为产病毒细胞PA317TK,用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞,命名为C6TK细胞,对GCV和ACV的敏感性分别高于亲本450倍和10倍;成功地建立了大鼠脑胶质瘤模型,并证实转染细胞C6TK的成瘤效应未改变,存活期约为15天;而经ACV治疗后,含有C6TK细胞的肿瘤生长明显被抑制,大鼠生存期延长为57.8±8.07天,尤其是采用PA317TK细胞混和治疗组和原位治疗组,肿瘤基本消失,大鼠生存期显著延长,混和治疗组存活120天以上,原位治疗组存活至71.4±36.1天。t检验,P值均小于0.001。

Results: GINaTK retroviral vector containing HSV-tk was constructed and transduced into PA317 retrovirus packaging cell by lipofectin (named PA317TK). Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P <0.001.

结果:构建了带有HSV-tk基因的反转录病毒载体GINaTK,应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317,成为产病毒细胞PA317TK,用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞,命名为C6TK细胞,对GCV和ACV的敏感性分别高于亲本450倍和10倍;成功地建立了大鼠脑胶质瘤模型,并证实转染细胞C6TK的成瘤效应未改变,存活期约为15天;而经ACV治疗后,含有C6TK细胞的肿瘤生长明显被抑制,大鼠生存期延长为57.8±8.07天,尤其是采用PA317TK细胞混和治疗组和原位治疗组,肿瘤基本消失,大鼠生存期显著延长,混和治疗组存活120天以上,原位治疗组存活至71.4±36.1天。t检验,P值均小于0.001。

The contents of N, P, K in leaves were examined by regular leaf analysis. The results showed that the N content was higher in leaf blade than in leafstalk. N content in leaf was gradually reduced along with the vine growth. N, F, K fertilized at 20 to 40 cm soil layers significantly increased N content in leaf. But N content in leafstalk was less affected by N fertilization depth and growing season. P content was higher in leafstalk than in leaf blade. Less change of P content was noticed in leaf along with vine growth. However, N, P, K fertilized at 40 to 60 cm soil layers significantly promoted P in both leaf blade and leafstalk. K content in leafstalk was much higher than in leaf Wade. No obvious influence was noticed by fertilizing depth. But K content in leafstalk was significantly increased due to applied K fertilizer in late growing season.

结果表明,酿酒葡萄叶片中氮含量远高于叶柄,叶片中氮素含量随着生育期的延长逐渐降低,而叶柄中氮含量随施肥深度增加和生育期延长而产生的变化很小,40cm的施肥深度能显著增加叶片中氮素含量;叶柄磷素平均含量高于叶片,全生育期叶片磷含量变化很小,而施肥60cm深度显著促进了叶片和叶柄中磷含量的提高;叶柄中钾含量远高于叶片,施肥深度对叶柄和叶片中钾含量未产生明显影响,但生育后期追施钾肥显著提高叶片钾含量;施肥深度达到40cm时酿酒葡萄的产量最高,而60cm的施肥深度产量显著下降。

With the raw material and the particle size of reducing agent narrowed, the percent reduction of strontia increases.(6) With the barbecuing pressure increasing, the percent reduction of strontia increases, after the certain value, the percent reduction of strontia decreases instead.(7) With reduction temperature increasing, the percent reduction of strontia increases.(8) The percent reduction of strontia is increasing with prolonging reduction time. In this experimental condition, it will become steady after 2.5h.

通过实验研究确定以SrCl_2-SrF_2-SrCO_3的纯锶盐体系作为电解质,实验中还采用了我们最新研制的熔盐电解监控仪对电解过程进行测量和系统的研究,得出以下结论:(1)增加电流强度,反电动势亦随之增加,而升高温度反电动势则有所降低;(2)随着电流密度的升高和电解时间的延长,电流效率逐渐增加,达到峰值后稳步下降;(3)延长电解时间,合金中锶的含量逐渐增加,最高可达18.1%;(4)开始电解后,反电动势逐渐增加,但向熔体中加入SrCO_3后,反电动势明显降低,因此认为在正常电解时是SrCO_3在分解。

The samples of 600℃ group didn't crystallize, but the color became darker and yellower as the peolongation of the heating time; the samples of 650℃ and 700℃ groups crystallized in different degrees, the translucency and Chroma decreased as the prolongation of the heating time, but the Value increased.

600℃组各样本均未晶化,随着保温时间的延长,其颜色逐渐加深,偏黄,650℃及700℃组各样本均发生不同程度的晶化,随晶化温度的升高及保温时间的延长,样本的透明度逐渐降低,亮度逐渐升高,色调变浅且饱和度逐渐降低

Edit in what defend Jian measure respect, the main opinion that gathers before this includes: It is to suggest not to lengthen the orgnaization that protect Jian to doing poineering work board the superintend and director of the company guides period, card inspect can think through research, because do poineering work board company dimensions is relatively lesser, management stability is relatively inferior, lengthen superintend and director to guide appropriately period be helpful for active and reliable roll out do poineering work board, reason did not grant to adopt; 2 it is to suggest exempt does poineering work board company IPO in those days namely the responsibility of the person that protect Jian of deficit, card inspect can think via research, although do poineering work board the company is managed not stable, but if the company appears on the market in those days with respect to deficit, can produce baddish effect to the market, because this cannot loosen the responsibility of pair of people that protect Jian to find out; 3 it is to suggest to unlock person of the delegate that protect Jian to sign odd number to measure limitation, meeting general is keeping next card watch in be being arranged related Jian system, give further research, hear opinion of orgnaization of Jian of be bailed out, make arrangement jointly.

在保荐办法的修订方面,此前收集到的主要意见包括:一是建议不要延长保荐机构对创业板公司的督导期,证监会经过研究认为,由于创业板公司规模相对较小,经营稳定性相对较低,适当延长督导期有利于积极稳妥推出创业板,故未予采纳;二是建议豁免创业板公司IPO当年即亏损的保荐人责任,证监会经研究认为,尽管创业板公司经营不稳定,但如果公司上市当年就亏损,对市场会产生较坏的影响,因此不能放松对保荐人的责任追究;三是建议放开保荐代表人签单数量限制,下一步证监会将在保荐制度相关安排中予以进一步研究,听取保荐机构意见,共同做出安排。

Suitable operation combination, bone shaft lengthening nearing bone end, keeping endosteal cortical bone osteotomy, and a continual lengthening of 1.0 mm.d- for adults and 1.5~2.0 mm.d-1 for children can get ideal limb length, quality and fast callus development and satisfactory joint function.

实施靠近骨端的骨干延长,保留内骨膜的皮质骨截骨,成人每天1.0 mm、儿童每天1.5~2.0 mm的连续延长,可获得理想的肢体长度、良好的骨痂生长速度和质量以及满意的关节功能。

Suitable operation combination, bone shaft lengthening nearing bone end, keeping endosteal cortical bone osteotomy, and a continual lengthening of 1.0 mm .

实施靠近骨端的骨干延长,保留内骨膜的皮质骨截骨,成人每天1.0 mm、儿童每天1.5~2.0 mm的连续延长,可获得理想的肢体长度、良好的骨痂生长速度和质量以及满意的关节功能。

Tissue were taken from intestine before and at 2h, 4h, 6h after operation, placed in 10% formalin, reviewed by a histopathologist.: Intestinal ischemia was noted with worsening grades of injury observed with greater ligation times.

结果:随着缺血时间的延长,肠壁的缺血损害程度逐渐加剧。D-乳酸的活性在术后2小时就随着缺血时间的延长,肠壁组织缺血程度的增加而逐渐升高,与手术前相比,P.05。

It is observed that the evaluation of sensory quality decrease with storage time and increasing temperature. It shows from the reliability assessment between predicted and observed shelf-life that relative error is within ±10% calculated by the prediction model for the shelf-life of Trichiurus haumela.

结果表明,鲜带鱼的总菌落数、总挥发性盐基氮、鲜度指标随着贮藏时间的延长而增加,且随着贮藏温度的升高而增加迅速,其感官品质指标随着贮藏时间的延长而下降,且随着贮藏温度的升高而下降迅速。

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