延长时间

PURPOSE: Clostridium difficile diarrhea is common in elderly patients and leads to prolonged hospitalization.

目的：难辨梭状芽胞杆菌感染型腹泻在老年患者中常见并可使患者的住院时间延长。

However, in actual use, its performance and air tightness due to the extension of time, dust and ink residues in the mouth to reduce the increase in coagulum.

但在实际使用中，它的性能及气密性会因时间的延长、灰尘及墨水在此嘴的残留凝固物增加而降低。

The bubbles grow up, coarsen and rupture with prolonging foaming time.

随着发泡时间的延长，泡沫Al中的气孔发生长大、粗化、崩塌。

With raising temperature and prolonging time of aging treatment, the TiC films coarsen gradually into block so that the notch sensitivity vanished from the alloy.

合金的缺口敏感性与薄膜状TiC沿晶界析出有着密切的关系，随着时效温度的提高和时效时间的延长，晶界薄膜状TiC逐渐变为块状，因而缺口敏感得以消除。

The second was to compare the effects of the different floating liquids on the destruction of rabbit coccidia oocysts. The results indicated that magnesium sulfate solution showed the lowest rate of coccidia oocyst destruction at 20 min; saturated salt water had the greatest rate of destruction. The destruction increased with time; in the saturated salt water and urea solution damage or deformation of a few coccidia oocysts at were observed 60 min.

其二，不同漂浮液对兔球虫卵囊破坏作用比较；结果表明20min时硫酸镁溶液对卵囊破坏率最低，破坏率最大的是饱和食盐水，随着时间延长各种破坏作用增大，60min时饱和食盐水和尿素溶液中有极少卵囊破损和卵囊变形现象。

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

To open, tear off on arrow line. Add 200cc boiled water into opened coffee bag. Or add water to cup and dip bag into hot water 3-4 times. Then let coffee bag seep in water up to 3-5 minutes. For stronger taste of coffee, seep longer.

将滤泡袋延箭头撕开、取热开水冲入袋中约200cc，或冲入滤泡袋热水3-4次、浸泡约3-5分钟、喜爱浓郁可延长浸泡时间。

IAA solution of 10μg/g greatly promoted the elongation and proton extrusion of maize coleoptile segments.

10μg／g的IAA溶液强烈地促进玉米胚芽鞘切段的延长生长和质子分泌，但这两种效应的启动时间有别。

Results The expression of EDA gene could be induced in E. coli. DH5α.

随着诱导时间的延长，EDA基因的表达量增加。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。