底酵母

Continuous ethanol fermentation using self-flocculating yeast SPSC01, a fusant from Saccharomyces cerevisiae and Schizosaccharomyces pombe, and two-stage enzymatic hydrolyte of dry milling corn powder, was continuously run for 120 days.

在一套四级串联悬浮床生物反应器系统中，以双酶法制备的玉米粉糖化液为底物，进行了废糟液全循环条件下自絮凝颗粒酵母乙醇连续发酵的实验研究。

The corn straws that it was treated with the ammonia taken for substrate, the TB9704 and feed yeast carried out the multi strain co fermentation in the orthogonal experiment, the two groups optimum combination of the co fermentation and technological processes and conditions were sifted.

在以氨法处理过的玉米秸秆为底物的TB9704与饲料酵母的混合菌共发酵的正交实验中，优选出2组最佳的共发酵的组合和工艺路线及条件。

The ubiquitin proteolytic system is an important selective proteolytic system of the celler proteolytic systems, a number of them were identified as a ubiquitin chain assembly factor-E4. The U-box protein is a new family ubiquitin-protein ligase(E3), which determine the substrate specificity of ubiquitination. The U-box is a domain of 70 amino acids that is highly conserved among yeast, plant and animals, but more U-box proteins were found in plants than in animals.

泛素系统是选择性降解细胞内蛋白质的重要系统之一，U-box蛋白质是此系统中决定底物特异性识别的一种新型E3蛋白质，部分U-box蛋白质属于泛素链聚集因子-E4.U-box结构域大约由70个氨基酸残基构成，在酵母、植物和动物等真核生物中保守存在，但植物中的数目远多于动物中。

This illustrated that GFP gene was expressed in the yeast ceil.

与其他酵母评价体系相比，以GFP为报告基因评价EEH不需要破坏细胞壁，也不需要底物，可在96孔板中完成，这为高通量筛选EEH化合物奠定了基础。

In the presence of DMSO as co-solvent and under vigorous agitation, baker\'s yeast in water was found to reduce substituted fluorenones to the corresponding fluorenols in good to excellent enantioselectivies (52%~ 100% ee). The experimental results clearly revealed that when mass transfer limitations are overcome, baker\'s yeast is able to reduce even highly sterically hindered ketones, such as substituted fluorenones.

在强烈的搅拌、并使用DMSO作为底物助溶剂的反应条件下，面包酵母对取代芴酮显示出较高得还原活性，得到立体选择性很高的相应的芴醇(52%～100%ee)。

First,through monofactorial experiment,we studied the influences of pH,temperature and substrate concentration on acidic trehalase activity of baker's yeast.

首先通过单因素实验研究了pH值、温度、底物浓度对面包酵母酸性海藻糖酶活性的影响，然后通过正交试验得出了酸性海藻糖酶活性测定的适宜条件：海藻糖浓度1.6%、温度30℃、pH 4.0、酵母菌泥接种量为0.02g/mL。

First, through monofactorial experiment, we studied the influences of pH, temperature and substrate concentration on acidic trehalase activity of baker's yeast. Then we designed an orthogonal experiment to detective the optimal condition of ascertaining acidic trehalase activity. The optimal conditions are: substrate concentration 1.6%, pH 4.0, temperature 30 and seed volume 0.02g/mL compressed yeast.

首先通过单因素实验研究了pH值、温度、底物浓度对面包酵母酸性海藻糖酶活性的影响，然后通过正交试验得出了酸性海藻糖酶活性测定的适宜条件：海藻糖浓度1.6%、温度30℃、pH 4.0、酵母菌泥接种量为0.02g/mL。

We first found out the preparation of intact budding yeast nuclei suitable for substrates for replication in Xenopus egg extracts.

我们首先摸索出了一个制备适用于爪蟾卵复制底物的完整芽殖酵母细胞核的方法。

The effect of mixed culture solid fermentation of Candida tropicalis ZD-3 and Aspergillus niger ZD-8 on detoxification of cottonseed cake,as well as the effect of sodium carbonate and minerals on the reduction of free gossypol and crude protein contents were investigated during the mixed culture fermentation of CSC by detection of protein disappearance rate in vitro.

通过测定发酵底物中游离棉酚、粗蛋白质含量，体外法测定饲料蛋白质消失率等方法研究热带假丝酵母ZD-3(Candida tropicalisZD-3)与黑曲霉ZD-8(Aspergillus nigerZD-8)复合固体发酵对棉籽饼粕脱毒效果的影响；碳酸钠以及矿物质处理对棉籽饼复合发酵脱毒效果的影响。

Could keep above 95% in the fixed beds. In order to achieve high stereo-selectivity with yeast, ethyl acetoacetate was used as the model substrate,and a novel approach was proposed to selectively inhibit R-enzymes in yeast before reaction.

以乙酰乙酸乙酯为模型底物，以丙烯酸、乙醚、二甲基亚砜、丙烯酰胺、正己烷等有机溶剂选择性地抑制酵母中R型酶，提高反应立体选择性。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失