应用生物学

This paper systematically analyzed the problems China faced in this field,and summarized the latest research achievements on bryophyte's biological and ecological characteristics.

在分析我国边坡植被生态恢复面临问题的基础上，系统综合目前有关苔藓植物生物学生态学特征的研究成果，结合对成都附近铁路边坡自然植被中苔藓植物进行的案例调查与分析，讨论了将苔藓植物应用边坡植被生态恢复的意义、问题，提出促进苔藓植物在边坡植被生态恢复中应用的途径。

As the development of monoclonal antibody and quantitated fluorescent cytochemistry technique, FCM has been used more extensively in biology, clinical medicine, pharmacology, material and so on.

本文就流式细胞术的发展历史、流式细胞仪的原理及在各领域的应用进行综述，阐明现代流式细胞术由于结合单克隆抗体技术、定量荧光细胞化学技术，使其在生物学、临床医学、药物学、材料学等众多研究领域中的应用有更加突飞猛进的发展。

Besides its convenience used in development research, the application in ethology is more and more extensively.

斑马鱼在神经生物学中的应用，除了在发育方面比其他模式动物更具优势外，在行为学方面的应用也更加丰富。

Because of its illuminant characteristic, the applied perspective of GFP is extensive in scientific research, biology, medicine and the other fields.

由于其具有发光的特性，已成为在生物化学和细胞生物学中研究和开发应用得最广泛的蛋白质之一，因而在科研及生物医药等领域具有良好的应用前景。

The others related to the development of immunological , cellular and molecular biology .

学的发展，以及各种高新技术的发明和应用，一些先进的实验技术和检测手段已经用于病理研究和对疾病的病理诊断中，并广泛应用于医学生物学研究领域。

The development and applications of SSR molecular markers in identification,hybridization and introgression,bottleneck effect in the invasion process of invasive insects were discussed in this paper.

本文概述了SSR分子标记在入侵昆虫来源鉴定、杂交和基因渗透、瓶颈效应等方面的应用进展，并对其在入侵生物学中的应用前景进行了展望。

Department of Systems Science and Mathematics Math.

刊载计算机在数值分析、概论率、数论、人工智能等数学研究中的使用，计算机的数学模型，计算机与数学相互应用及其在环境科学、生态学、生物学和城市系统领域的应用方面的原始论文、评论等。

He existence and stability of the nontrivial and nonnegative steady-state solution of an ecological predator-prey system with the reaction-diffusion term are discussed.

应用渐近展开的方法构造生物学里提出的一类具有反应扩散项的捕食者─食饵系统的非平凡非负定态解，并应用固有值的解析摄动理论对所获得的非平凡非负定态解进行了稳定性分析。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

The researches cover systemic microbiology, microgenetics, applied microbiology, cells biology of virus infection and cryobiology.

保藏的培养物包括细菌，酵母菌，真菌，转基因细胞，原生动物，地衣和植物种子等等，研究项目包括系统微生物学，微生物遗传学，应用微生物学，病毒感染细胞生物学和低温生物学。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。