应用生物学

The journal publishes original research papers, short reports, reviews, lectures and book reviews in the field of biosocial science, the common ground between biology and sociology, including social and biological aspects of reproduction and its control, gerontology, ecology, genetics, applied psychology, sociology, education, criminology, demography, health and epidemiology.

杂志刊载原始研究论文，短报告，评论，演讲和书评，内容涉及生物社会科学，生物学和社会学共同性领域，包括生育的社会和生物学方面及其控制，老年学，生态学，遗传学，应用心理学，社会学，教育，犯罪学，人口统计学，卫生和流行病学。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

The phylogenetic analysis of Gymnothorax was reported in this paper by being based on DNA sequences of mitochondrial Cytb gene.17 species of Cytb gene full-length sequences were obtained and their length of Cytb gene was 1140bp.All sequences were analysised by using biological software, the phylogenetic tree was constructed by using three kinds of common contribution phylogenetic tree methods and the phylogeny of 17 species of Gymnothorax was deduced.

本论文采用线粒体Cytb基因为分子标记，共获得裸胸鳝属鱼类17种Cytb基因序列全长为1140bp的部分序列，应用分子生物学软件对结果进行分析，使用3种常见的建树方法：NJ法、ME法和MP法重建系统发育树，对裸胸鳝属17种鱼类的系统关系进行研究，为探讨裸胸鳝属鱼类的分子进化，确定它们之间的关系提供分子生物学方面的证据。

Objective: The experiment will to observe: 1、the compound composed of carriers and rhBMP-2 excels or is similar to autologous iliac crest in posterolateral intertransverse spine arthrodesis in rabbits, 2、it may eliminated thd nonuions, 3、To explore the biologic process in spine arthrodesis and the role that accelaterated spine arthrodesis, 4、the roles of HA in spine fusion, by those compounds are used in posterolateral intertransverse fusion in rabbits.

目的：本实验通过rhBMP-2、HA复合物在兔子腰椎后外侧横突间融合的过程，观察了解：（1）rhBMP-2复合物在兔子腰椎后外侧横突间融合中，其作用能否优于或至少与自体髂骨相同，能否取代自体髂骨，免去取骨术。（2） rhBMP-2复合物的应用能否消除不融合的发生。（3）进一步了解脊柱融合的生物学过程，及rhBMP-2在脊柱融合生物学过程中所起的作用。（4）探讨HA在脊柱融合中的作用。

The experiment on electromagnetism pulse biology effect could suggest that quantities-effect of EMP effect on system morphemic and function with applying different frequency, power and expose time. Combined with epidemic investigation and medical psychological research in EMP researcher, the immediate, early stage and middle and long-term biological effect of EMP was elucidated and EMP protection standard was established.

电磁脉冲(electromagnetism pulse，EMP)生物学效应实验是通过应用不同频率、功率、暴露时间的EMP照射动物，明确EMP所致各系统形态和功能危害的量效关系，探索其效应机理；并结合对EMP从业人员的流行病学调查和医学心理学研究，揭示EMP所引起的即刻、早期、中期和远期生物学效应，提出EMP卫生防护标准，为物理防护和医学防护的研究提供重要的理论依据和实验依据。

The researchers can get the relative information quickly by collecting, organizing and managing the data and then use the information expediently. We can gain the deep-seated biological knowledge by dealing with and digging the data and then enhance our understanding to the biology world. In the applications and researches of biology and iatrology we can improve the efficiency and scientize the researches and exploitation by using the data and analysed results. Just like to examine the related genes of some disease basing on the result of analysing the genes' fuctions and to design a new medicine basing on the results of analysing the protein.

通过收集、组织、管理生物分子数据，使研究人员能够迅速地获得和方便地使用相关信息；通过处理、分析、挖掘生物分子数据，得到深层次的生物学知识，加深对生物世界的认识；在生物学、医学的研究和应用中，利用生物分子数据及其分析结果，可以大大提高研究和开发的科学性及效率，如根据基因功能分析结果来检测与疾病相关的基因，根据蛋白质分析结果进行新药设计。

In this thesis,using the methods of organic chemistry and cell biology the cross topic about thebiological applications of fullerene derivatives was explored.

本文利用有机化学和细胞生物学的方法对富勒烯衍生物的生物学应用这一交叉课题进行了探索。

They have many years of scientific research-based, application of cell biology and molecular biology methods, further research and development of new hair tonic of raw materials, and to global problems - the male sex hormone hair loss as the main target.

他们以多年的科研成果为基础，应用细胞生物学与分子生物学的方法，进一步研究和开发新型生发剂原材料，并把世界性难题--雄性激素性脱发列为主攻目标。

The journal publishes original research on the evaluation and characterization of teratogens, carcinogens, or mutagens; reports describing the development of novel experimental techniques for toxicological assessment, or the application of current methods in molecular and cellular biology (including in vitro studies, organ and cell culture, and the use of transgenic animals); as well as critical reviews focused on topics of significant interest to specialists in teratology, cancer biology, and mutation research.

杂志刊有关载致畸剂，致癌物质和诱变剂的评估和特征方面的原始研究；描述毒物评定的实验新技术，或当前方法在分子和细胞生物学（包括体外研究，器官和细胞培养，和转基因动物）中应用的报告；以及对畸形学，癌生物学，和突变研究中生物学家非常感兴趣课题的评论。

The cryobiology has been applied for breeding, preservation of genetic resources, and development of new strains of laboratory animals.

随着低温生物学理论的不断深入及其技术的不断完善，低温生物学技术已被广泛应用于实验动物的生产、遗传资源的保存及新品系开发等领域。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。