应用生物学

According to the characteristics of totipotency , differentiation out of body and the steady transmissibility of the ES cells, we can see wide application prospects of the ES cells which could be used to build the model of early diffierentiation out of body and the model of transgenic animal ,to restore apparatus and tissue and transplant therapy, to produce clone animal and research .

人们利用ES细胞所具有的全能性、体外分化以及稳定的遗传性能等特点，展示了ES细胞在建立哺乳动物的早期胚胎体外分化模型、转基因动物模型、器官和组织的修复与移植治疗、克隆动物的生产、发育生物学的研究等方面广阔的应用前景。

Primers with clear and abundant polymorphisms bands were selected from 190 primers with the established ISSR-PCR procedure which could be used to analyze ISSR molecular biology of Trichosanthes kirilowii Maxim.

经验证，本实验所建立的栝楼ISSR-PCR反应体系具有稳定可靠、可重复性好、多态性较强等特点，能够较好的应用于栝楼的ISSR分子生物学分析。

The osteogenesis was observed by HE staining, modified Mallory's trichrome staining, and image analysis.

随着生物材料、细胞生物学的发展，使应用组织工程技术修复组织缺损成为可能。

Glucocorticoids are effective in inducing apoptosis via the uncharacterized mechanisms in many hematological malignancies. GC-induced apoptosis is initiated by, and strictly dependent upon, the interaction of GC with its receptor, the GR.

糖皮质激素(glucocorticoid，GC)通过与其特异性受体-糖皮质激素受体(glucocorticoid receptor，GR)结合，不仅可诱导未成熟胸腺细胞凋亡，而且对急性白血病细胞、淋巴瘤细胞、骨髓瘤细胞等均有促凋亡作用，诱发凋亡正是应用糖皮质激素治疗白血病的分子生物学基础。

Methods: On the basis of cloning the M-TNF c D NA encoding region,the authors constructed mTNFm,which could potentially expre ss on the c ell surface with a uncleavable type of TNF resulting in deletion of the sequence encoding enzymatic cleavage site of TNF-α-converting enzyme.

近年来研究表明，T NF的两种形式在受体结合、生物学效应等方面各具特征［3，4］。M-TNF通过直接接触在组织局部中发挥效应的特性，有望克服S-TNF在生物治疗应用中引起的远端及全身性毒副作用。

A kind of M-TNF recombin ant with a stable,uncleavable mutant typewas constructed for its gene therap y. Methods: On the basis of cloning the M-TNF c D NA encoding region,the authors constructed mTNFm,which could potentially expre ss on the c ell surface with a uncleavable type of TNF resulting in deletion of the sequence encoding enzymatic cleavage site of TNF-α-converting enzyme.

近年来研究表明，T NF的两种形式在受体结合、生物学效应等方面各具特征［3，4］。M-TNF通过直接接触在组织局部中发挥效应的特性，有望克服S-TNF在生物治疗应用中引起的远端及全身性毒副作用。

DNA single-strand breaks in peripheral blood lymphocytes of 20 normal pepole,17workers exposed to external irradiation,28 unirradiated and 19 irradiatedpatients with malignant tumor were studied by comet assay.

应用碱性单细胞凝胶电泳技术检测了正常健康人、肿瘤患者、X射线接触者和肿瘤放疗病人外周血淋巴细胞的DNA单链断裂，探讨其单独作为潜在的人体健康危害早期生物学指标的可能性。

The structure,processing characteristics,biolog ical function of functional factors in colostrums and immune milk and their application,in food industry are reviewed,and the development directions of functional factors are presented in this paper.

综述了初乳与免疫乳中功能成分的结构特性、加工特性、生物学功能以及在食品领域中的应用，并提出了开发牛乳中功能成分的方向。

PNA hybridizes with DNA or RNA by means of Waston Cricks base pairs complementary with high stability, affinity and selectivity. PNA not only regulates DNA replication, but also adjusts DNA transcription and translation. Many applications have been explored as a new kind of molecular biological tool and a gene targeting strategy.

肽核酸是一类人工合成的核酸类似物，PNA与核酸链以 Waston- Crick碱基配对方式稳定互补结合，具有高度的亲合性、稳定性、特异性特征，PNA能调节基因的表达作用，包括调节基因的复制、转录、翻译过程，因此 PNA有着广泛的分子生物学效应，显示出作为基因调节药物的应用潜力

BACKGROUND: As an ideal scaffold of cartilage tissue engineering, demineralized bone matrix has been widespread used.

背景：脱钙松质骨被认为是一种&理想&的软骨组织工程支架材料已被广泛应用，但对其某些生物学特征目前仍不是十分清楚。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。