应用生物学

High-level expression of secreted Flt-1(2-3) with good bi ological activity were successfully achieved in Pichia.pastoris expression syst em and can be applied to further animal and clinical test.

获得了具有生物学活性的可溶性重组F lt-1受体胞外区2-3loop小片段，为进一步的动物实验和临床应用打下了基础。

Whether characteristics of periosteal osteoblasts after cryopreservation and thawing can be maintained and used in clinic need further explored.

冻存复苏后的生物学特点是否能进行进一步临床应用研究有待证实。

The morphology and phenological characters were described combination with ornament and garden's needs.

结合观赏角度和园林应用方面，对百合的形态特征和生物学特性等进行描述。

But the effection of expressional phytase for bearing high temperature and standing the acid was not significant.

植酸酶是一种生物学效应广泛的营养因子，在实际生产和应用中，人们更希望它具有满足工业化生产的酶学特性。

Porphyra polysaccharide has been widely applied to food,medicine,zoo nutrition industry etc because of its special biological functions.

紫菜多糖具有多种独特的生物学功能，在食品、医药、动物营养等领域具有广阔的应用前景。

We used transmission electron microscopy to study the ultrastructural changes in egg cell, zygote and two-celled proembryo of Beta vulgaris to provide information on the reproductive biology of angiosperms and related research into sugar beet.

为丰富被子植物生殖生物学资料，并为甜菜相关研究提供参考，应用透射电镜技术研究栽培甜菜卵细胞、合子和二细胞原胚的超微结构特征。

To study furderly the activity of CNTF mutant designed by computer molecular modling,this study used the methods of dissociated cultures of chick dorsal root ganglion、TF-1 prolification and the normal mice′weight loss tests.

为了进一步研究我室应用计算机分子模拟设计并表达纯化的睫状神经营养因子突变体蛋白的生物学活性，分别采用鸡胚背根神经节无血清培养法、TF-1细胞增殖法、正常小鼠减重法对其活性进行研究。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

In this paper,the research progress on psychrophilic methanogens was reviewed,with the focus on thei...

本文针对研究者广泛关注的热点问题，从分离培养及生理生化特性、适冷机制和分子生物学研究几个方面，对嗜冷产甲烷菌的研究进展进行了全面的综述，并对其在低温厌氧生物处理技术中的应用前景进行了分析和展望。

In this paper, the research progress on psychrophilic methanogens was reviewed, with the focus on their culture, isolation, physiological and biochemical characters, molecular biological characters, and cold -adapted mechanisms. The application prospects of psychrophilic methanogens in low-temperature anaerobic technology were also analyzed.

本文针对研究者广泛关注的热点问题，从分离培养及生理生化特性、适冷机制和分子生物学研究几个方面，对嗜冷产甲烷菌的研究进展进行了全而的综述，并对其在低温厌氧生物处理技术中的应用前景进行了分析和展望。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。