应用化学

Methods Sixty adult healthy male Wistar rats were enrolled in the experiment. PTSD-like rat model was established by single prolonged stress. There was no SPS stimulation in the control group and the rats of the other five groups were undisturbedly raised for 24 hours, 4 days,7 days,14 days and 28 days respectively after SPS stimulation.The expressions of glucocorticoid receptors in the locus ceruleus nucleus of all groups were detected with Nissl staining, immunohistochemistry,Western blotting and PCR, and image analysis and statistical analysis were performed.

成年健康雄性Wistar大鼠60只，使用无连续单一应激方法建立PTSD大鼠模型，分别取SPS处理后24h、4d、7d、14d和28d大鼠蓝斑组织，同时取正常蓝斑组织作为对照，应用尼氏染色观察蓝斑形态，采用免疫组织化学、免疫印迹和RT-PCR方法分别观察及检测各组蓝斑神经元GR表达的变化，并进行图像分析和统计学处理。

In recent years, some non-metallic anti-corrosion materials, such as plastic, fiberglass, glass, ceramic, unpenetrable graphite and so on, are widely used in chemical production and have obtained remarkable effects.

近年来，一些非金属防腐材料，如塑料，玻璃钢，玻璃，陶瓷和不透性石墨等，在化学生产中得到的广泛的应用，取得了显着的效果。

The electronic structures and the non-linear optical properties of bis schiff base and their derivatives have been studied using FF/HF method.The effects of electron donor/acceptor on nonlinear optical property were examined at the molecular level.As a result,the NLO coefficients of the substituted compounds are all larger than the unsubstituted compound.Among them, the β value of compound 4 is the largest one.

自1961年激光发现以来，非线性光学和非线性光学材料迅速发展起来[1～4]，随着光通讯、光电子学和光信息处理等在实际中的应用，对非线性光学材料的理论与实验研究进一步展开，寻找和设计具有较大非线性光学系数，超快光学响应，较低的光学损伤阈值，良好的化学稳定性和热稳定性的非线性光学材料成为这一领域的主题[5～12]。

Results; The histological change of kidney iNOS was markedly increased in the IR+NS group. The levels of serum creatinine and blood uria nitrogen were significantly upregulated.

应用免疫组织化学方法和图像分析系统检测肾组织中INOS的表达和分布，比较各组血清肌酐、尿素氮，同时观察肾脏组织形态学改变。

As MEMS (Micro Electro-Mechanical Systems) technology progresses rapidly, the synergism between MEMS and biomedical technology becomes closer than ever before. When the uTAS was introduced in the last two decades, many microfluidic chips, microelectrodes, and microreactors are being studied for drug detection, environment detection, gene analysis etc. Due to its small volume, low pollution, low consumable and good portability, uTAS plays a very important role in biomedical, biochemical, environment engineering fields.

随着MEMS(Micro Electro-Mechanical System，微机电系统)制造技术的不断发展，生物医学与其之间的联系越来越紧密，自上世纪90年代初提出uTAS(Micro Total Analysis System，微全分析系统)之后，各种各样的微流控芯片、微反应器、微电极、微分离器等便大量涌现，广泛应用于药物检测、环境监测、基因分析等诸多领域中，并且由于其试剂消耗量少、检测效率高、环境污染小、体积小、易于携带等优点，在生物医学、化学、环境工程等领域受到越来越多的重视。

Methods Western blotting and immunohistochemical staining were used to detect CD147 expression in cervical cancer or normal cervix uteri tissues.

应用蛋白印迹及免疫组织化学方法检测CD147在宫颈癌和正常宫颈组织中的表达。

Static light scattering can measure the osmotic second virial coefficient of protein solution.

光散射技术广泛应用于生物、化学等方面的研究，尤其对蛋白质溶液晶体生长的研究，体现了其独特的优势。

Micropump used as actuator of microfluid device is an important part of micro－ electro－ mechanical systems.

微型泵在生物化学分析、药物输送和芯片冷却等领域有广阔的应用前景。

Subjection of Nature's forces to man, machinery, application of chemistry to industry and agriculture, steam-navigation, railways, electric telegraphs, clearing of whole continents for cultivation, canalisation of rivers, whole populations conjured out of the ground -- what earlier century had even a presentiment that such productive forces slumbered in the lap of social labour?

自然力的征服，机器的采用，化学在工业和农业中的应用，轮船的行驶，铁路的通行，电报的使用，整个整个大陆的开垦，河川的通航，仿佛用法术从地下呼唤出来的大量人口，----过去哪一个世纪料想到在社会劳动里蕴藏有这样的生产力呢？

In particular the atmospheric pressure chemical ionisation and the electrospray ionisation methods are nowwidely used in routine analysis (see also section on PTR-MS and HOx radicals below).

尤其大气压化学电离和电喷雾电离方法现在广泛应用于常规分析（请参见PTR 和 HOx 下有关基础）。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。